LOCUS BBa_K1937001 5857 bp DNA linear 01-Jun-2024 DEFINITION pSBBsOK-mini, a small replicative plasmid for Bacillus subtilis ACCESSION BBa_K1937001 VERSION BBa_K1937001.1 FEATURES Location/Qualifiers CDS 5202..5600 /label=Bleomycin resistance (B. subtilis) CDS 1564..2223 /label=Ampicillin resistance (E. coli) misc_feature 784..1466 /label=ColE1 origin (E. coli) CDS 4209..4979 /label=Kanamycin resistance (B. subtilis) misc_feature 257..278 /label=Prefix misc_feature 285..305 /label=Suffixe misc_feature 3045..4049 /label=repU (Bacillus subtilis origin) primer_bind 2546..2565 /label=Verification forward (VF2) primer binding site primer_bind 685..704 /label=Verification reverse (VR) primer binding site ORIGIN 1 atctattatt aatctgttca gcaatcgggc gcgattgctg aataaaagat acgagagacc 61 tctcttgtat cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca 121 ataaaaattt tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata 181 aaaattggca agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc 241 tgtcccttgc tgatttgaat tcgcggccgc ttctagaggc tagctactag tagcggccgc 301 tgcaggcgca acgcaattaa tgtgagttag gatcctgcct cgcgcgtttc ggtgatgacg 361 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 421 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 481 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 541 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggaa 601 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 661 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 721 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 781 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 841 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 901 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 961 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 1021 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 1081 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 1141 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 1201 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 1261 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 1321 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 1381 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 1441 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 1501 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 1561 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 1621 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 1681 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 1741 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 1801 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 1861 cgttgttgcc attgctgccg gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 1921 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 1981 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 2041 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 2101 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 2161 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct 2221 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 2281 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 2341 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 2401 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 2461 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 2521 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 2581 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagagt tccttaagga 2641 acgtacagac ggcttaaaag cctttaaaaa cgtttttaag gggtttgtag acaaggtaaa 2701 ggataaaaca gcacaattcc aagaaaaaca cgatttagaa cctaaaaaga acgaatttga 2761 actaactcat aaccgagagg taaaaaaaga acgaagtcga gatcagggaa tgagtttata 2821 aaataaaaaa agcacctgaa aaggtgtctt tttttgatgg ttttgaactt gttctttctt 2881 atcttgatac atatagaaat aacgtcattt ttattttagt tgctgaaagg tgcgttgaag 2941 tgttggtatg tatgtgtttt aaagtattga aaacccttaa aattggttgc acagaaaaac 3001 cccatctgtt aaagttataa gtgactaaac aaataactaa atagatgggg gtttctttta 3061 atattatgtg tcctaatagt agcatttatt cagatgaaaa atcaagggtt ttagtggaca 3121 agacaaaaag tggaaaagtg agaccatgga gagaaaagaa aatcgctaat gttgattact 3181 ttgaacttct gcatattctt gaatttaaaa aggctgaaag agtaaaagat tgtgctgaaa 3241 tattagagta taaacaaaat cgtgaaacag gcgaaagaaa gttgtatcga gtgtggtttt 3301 gtaaatccag gctttgtcca atgtgcaact ggaggagagc aatgaaacat ggcattcagt 3361 cacaaaaggt tgttgctgaa gttattaaac aaaagccaac agttcgttgg ttgtttctca 3421 cattaacagt taaaaatgtt tatgatggcg aagaattaaa taagagtttg tcagatatgg 3481 ctcaaggatt tcgccgaatg atgcaatata aaaaaattaa taaaaatctt gttggtttta 3541 tgcgtgcaac ggaagtgaca ataaataata aagataattc ttataatcag cacatgcatg 3601 tattggtatg tgtggaacca acttatttta agaatacaga aaactacgtg aatcaaaaac 3661 aatggattca attttggaaa aaggcaatga aattagacta tgatccaaat gtaaaagttc 3721 aaatgattcg accgaaaaat aaatataaat cggatataca atcggcaatt gacgaaactg 3781 caaaatatcc tgtaaaggat acggatttta tgaccgatga tgaagaaaag aatttgaaac 3841 gtttgtctga tttggaggaa ggtttacacc gtaaaaggtt aatctcctat ggtggtttgt 3901 taaaagaaat acataaaaaa ttaaaccttg atgacacaga agaaggcgat ttgattcata 3961 cagatgatga cgaaaaagcc gatgaagatg gattttctat tattgcaatg tggaattggg 4021 aacggaaaaa ttattttatt aaagagtagt tcaacaaacg ggccagtttg ttgaagatta 4081 gatgctataa ttgttattaa aaggattgaa ggatgcttag gaagacgagt tattaatagc 4141 tgaataagaa cggtgctctc caaatattct tatttagaaa agcaaatcta aaattatctg 4201 aaaagggaat gagaatagtg aatggaccaa taataatgac tagagaagaa agaatgaaga 4261 ttgttcatga aattaaggaa cgaatattgg ataaatatgg ggatgatgtt aaggctattg 4321 gtgtttatgg ctctcttggt cgtcagactg atgggcccta ttcggatatt gagatgatgt 4381 gtgtcatgtc aacagaggaa gcagagttca gccatgaatg gacaaccggt gagtggaagg 4441 tggaagtgaa ttttgatagc gaagagattc tactagatta tgcatctcag gtggaatcag 4501 attggccgct tacacatggt caatttttct ctattttgcc gatttatgat tcaggtggat 4561 acttagagaa agtgtatcaa actgctaaat cggtagaagc ccaaacgttc cacgatgcga 4621 tttgtgccct tatcgtagaa gagctgtttg aatatgcagg caaatggcgt aatattcgtg 4681 tgcaaggacc gacaacattt ctaccatcct tgactgtaca ggtagcaatg gcaggtgcca 4741 tgttgattgg tctgcatcat cgcatctgtt atacgacgag cgcttcggtc ttaactgaag 4801 cagttaagca atcagatctt ccttcaggtt atgaccatct gtgccagttc gtaatgtctg 4861 gtcaactttc cgactctgag aaacttctgg aatcgctaga gaatttctgg aatgggattc 4921 aggagtggac agaacgacac ggatatatag tggatgtgtc aaaacgcata ccattttgaa 4981 cgatgacctc taataattgt taatcatgtt ggttacgtat ttattaactt ctcctagtat 5041 tagtaattat catggctgtc atggcgcatt aacggaataa agggtgtgct taaatcgggc 5101 cattttgcgt aataagaaaa aggattaatt atgagcgaat tgaattaata ataaggtaat 5161 agatttacat tagaaaatga aaggggattt tatgcgtgag aatgttacag tctatcccgg 5221 cattgccagt cggggatatt aaaaagagta taggttttta ttgcgataaa ctaggtttca 5281 ctttggttca ccatgaagat ggattcgcag ttctaatgtg taatgaggtt cggattcatc 5341 tatgggaggc aagtgatgaa ggctggcgct ctcgtagtaa tgattcaccg gtttgtacag 5401 gtgcggagtc gtttattgct ggtactgcta gttgccgcat tgaagtagag ggaattgatg 5461 aattatatca acatattaag cctttgggca ttttgcaccc caatacatca ttaaaagatc 5521 agtggtggga tgaacgagac tttgcagtaa ttgatcccga caacaatttg attagctttt 5581 ttcaacaaat aaaaagctat aatctattat taatctgttc agcaatcggg cgcgattgct 5641 gaataaaaga tacgagagac ctctcttgta tcttttttat tttgagtggt tttgtccgtt 5701 acactagaaa accgaaagac aataaaaatt ttattcttgc tgagtctggc tttcggtaag 5761 ctagacaaaa cggacaaaat aaaaattggc aagggtttaa aggtggagat tttttgagtg 5821 atcttctcaa aaaatactac ctgtcccttg ctgattt //