LOCUS       BBa_K1949033            2580 bp    DNA     linear       17-May-2024
DEFINITION  PBAD/araC-rbs-yafO-tt-Ptet-rbs-gfp
ACCESSION   BBa_K1949033
VERSION     BBa_K1949033.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..1635
                     /label=BBa_K1949030
     stem_loop       1655..1698
                     /label=stem_loop
     misc_feature    1644..1723
                     /label=BBa_B0010
     terminator      1644..1723
                     /label=BBa_B0010
     stem_loop       1739..1758
                     /label=T7 TE
     misc_feature    1765..1765
                     /label=stop
     polyA_site      1759..1772
                     /label=polya
     misc_feature    1732..1772
                     /label=BBa_B0012
     terminator      1732..1772
                     /label=BBa_B0012
     terminator      1644..1772
                     /label=BBa_B0015
     misc_feature    1781..1799
                     /label=TetR 1
     misc_feature    1781..1834
                     /label=BBa_R0040
     promoter        1800..1805
                     /label=-35
     misc_feature    1806..1824
                     /label=TetR 2
     promoter        1823..1828
                     /label=-10
     promoter        1781..1834
                     /label=BBa_R0040
     misc_feature    1847..1850
                     /label=conserved
     RBS             1843..1854
                     /label=BBa_B0034
     CDS             1861..2580
                     /label=GFP protein
     CDS             1861..2580
                     /label=BBa_E0040
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgc gggtattcaa aacaaaactt
     1261 attcgcctgc aacttacagc agaggaactt gatgcgttaa cggcggattt tatttcctat
     1321 aagcgtgacg gtgttttgcc agatatattt ggtcgcgatg cactctacga cgactccttt
     1381 acctggccat taatcaaatt tgagcgagtt gctcatattc atctggcaaa tgagaataat
     1441 ccatttccgc cacagttgcg ccaattcagc agaacgaatg acgaagcgca tttggtatat
     1501 tgtcaggggg cgtttgatga gcaagcatgg ttgctcattg ccattctgaa acctgaacct
     1561 cataaactgg ctcgagataa caaccaaatg cataaaattg ggaaaatggc agaagcgttt
     1621 cgcatgcgtt tttaatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1681 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1741 ctcaccttcg ggtgggcctt tctgcgttta tatactagag tccctatcag tgatagagat
     1801 tgacatccct atcagtgata gagatactga gcactactag agaaagagga gaaatactag
     1861 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
     1921 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
     1981 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
     2041 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
     2101 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
     2161 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
     2221 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
     2281 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
     2341 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
     2401 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     2461 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
     2521 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa
//