LOCUS       BBa_K195103             2004 bp    DNA     linear       01-Jun-2024
DEFINITION  RBS+Lpp-OmpA-linker+CD4 gene+terminator
ACCESSION   BBa_K195103
VERSION     BBa_K195103.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    21..27
                     /label=NdeI
     CDS             24..458
                     /label=OmpA
     CDS             459..484
                     /label=linker
     misc_feature    479..484
                     /label=SacI
     misc_feature    24..26
                     /label=ATG
     misc_feature    21..484
                     /label=BBa_K103006
     CDS             491..1867
                     /label=BBa_K195100
     stem_loop       1887..1930
                     /label=stem_loop
     misc_feature    1876..1955
                     /label=BBa_B0010
     terminator      1876..1955
                     /label=BBa_B0010
     stem_loop       1971..1990
                     /label=T7 TE
     misc_feature    1997..1997
                     /label=stop
     polyA_site      1991..2004
                     /label=polya
     misc_feature    1964..2004
                     /label=BBa_B0012
     terminator      1964..2004
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagag catatgaaag ctactaaact ggtactgggc gcggtaatcc
       61 tgggttctac tctgctggca ggttgctcca gcaacgctaa aatcgatcag ggaattaacc
      121 cgtatgttgg ctttgaaatg ggttacgact ggttaggtcg tatgccgtac aaaggcagcg
      181 ttgaaaacgg tgcatacaaa gctcagggcg ttcaactgac cgctaaactg ggttacccaa
      241 tcactgacga cctggacatc tacactcgtc tgggtggcat ggtatggcgt gcagacacta
      301 aatccaacgt ttatggtaaa aaccacgaca ccggcgtttc tccggtcttc gctggcggtg
      361 ttgagtacgc gatcactcct gaaatcgcta cccgtctgga ataccagtgg accaacaaca
      421 tcggtgacgc acacaccatc ggcactcgtc cggacaacgg cggaggttct ggaggaggga
      481 gctctactag atgaaccggg gagtcccttt taggcacttg cttctggtgc tgcaactggc
      541 gctcctccca gcagccactc agggaaagaa agtggtgctg ggcaaaaaag gggatacagt
      601 ggaactgacc tgtacagctt cccagaagaa gagcatacaa ttccactgga aaaactccaa
      661 ccagataaag attctgggaa atcagggctc cttcttaact aaaggtccat ccaagctgaa
      721 tgatcgcgct gactcaagaa gaagcctttg ggaccaagga aactttcccc tgatcatcaa
      781 gaatcttaag atagaagact cagatactta catctgtgaa gtggaggacc agaaggagga
      841 ggtgcaattg ctagtgttcg gattgactgc caactctgac acccacctgc ttcaggggca
      901 gagcctgacc ctgaccttgg agagcccccc tggtagtagc ccctcagtgc aatgtaggag
      961 tccaaggggt aaaaacatac agggggggaa gaccctctcc gtgtctcagc tggagctcca
     1021 ggatagtggc acctggacat gcactgtctt gcagaaccag aagaaggtgg agttcaaaat
     1081 agacatcgtg gtgctagctt tccagaaggc ctccagcata gtctataaga aagaggggga
     1141 acaggtggag ttctccttcc cactcgcctt tacagttgaa aagctgacgg gcagtggcga
     1201 gctgtggtgg caggcggaga gggcttcctc ctccaagtct tggatcacct ttgacctgaa
     1261 gaacaaggaa gtgtctgtaa aacgggttac ccaggaccct aagctccaga tgggcaagaa
     1321 gctcccgctc cacctcaccc tgccccaggc cttgcctcag tatgctggct ctggaaacct
     1381 caccctggcc cttgaagcga aaacaggaaa gttgcatcag gaagtgaacc tggtggtgat
     1441 gagagccact cagctccaga aaaatttgac ctgtgaggtg tggggaccca cctcccctaa
     1501 gctgatgctg agtttgaaac tggagaacaa ggaggcaaag gtctcgaagc gggagaaggc
     1561 ggtgtgggtg ctgaaccctg aggcggggat gtggcagtgt ctgctgagtg actcgggaca
     1621 ggtcctgctg gaatccaaca tcaaggttct gcccacatgg tccaccccgg tgcagccaat
     1681 ggccctgatt gtgctggggg gcgtcgccgg cctcctgctt ttcattgggc taggcatctt
     1741 cttctgtgtc aggtgccggc accgaaggcg ccaagcagag cggatgtctc agatcaagag
     1801 actcctcagt gagaagaaga cctgccagtg tcctcaccgg tttcagaaga catgtagccc
     1861 catttgatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1921 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1981 cgggtgggcc tttctgcgtt tata
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