LOCUS       BBa_K195203             3666 bp    DNA     linear       01-Jun-2024
DEFINITION  RBS+Lpp-OmpA-linker+alpha-V integrin gene+terminator
ACCESSION   BBa_K195203
VERSION     BBa_K195203.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    21..27
                     /label=NdeI
     CDS             24..458
                     /label=OmpA
     CDS             459..484
                     /label=linker
     misc_feature    479..484
                     /label=SacI
     misc_feature    24..26
                     /label=ATG
     misc_feature    21..484
                     /label=BBa_K103006
     CDS             491..3529
                     /label=BBa_K195200
     stem_loop       3549..3592
                     /label=stem_loop
     misc_feature    3538..3617
                     /label=BBa_B0010
     terminator      3538..3617
                     /label=BBa_B0010
     stem_loop       3633..3652
                     /label=T7 TE
     misc_feature    3659..3659
                     /label=stop
     polyA_site      3653..3666
                     /label=polya
     misc_feature    3626..3666
                     /label=BBa_B0012
     terminator      3626..3666
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagag catatgaaag ctactaaact ggtactgggc gcggtaatcc
       61 tgggttctac tctgctggca ggttgctcca gcaacgctaa aatcgatcag ggaattaacc
      121 cgtatgttgg ctttgaaatg ggttacgact ggttaggtcg tatgccgtac aaaggcagcg
      181 ttgaaaacgg tgcatacaaa gctcagggcg ttcaactgac cgctaaactg ggttacccaa
      241 tcactgacga cctggacatc tacactcgtc tgggtggcat ggtatggcgt gcagacacta
      301 aatccaacgt ttatggtaaa aaccacgaca ccggcgtttc tccggtcttc gctggcggtg
      361 ttgagtacgc gatcactcct gaaatcgcta cccgtctgga ataccagtgg accaacaaca
      421 tcggtgacgc acacaccatc ggcactcgtc cggacaacgg cggaggttct ggaggaggga
      481 gctctactag atggcttttc cgccgcggcg acggctgcgc ctcggtcccc gcggcctccc
      541 gcttcttctc tcgggactcc tgctacctct gtgccgcgcc ttcaacctag acgtggacag
      601 tcctgccgag tactctggcc ccgagggaag ttacttcggc ttcgccgtgg atttcttcgt
      661 gcccagcgcg tcttcccgga tgtttcttct cgtgggagct cccaaagcaa acaccaccca
      721 gcctgggatt gtggaaggag ggcaggtcct caaatgtgac tggtcttcta cccgccggtg
      781 ccagccaatt gaatttgatg caacaggcaa tagagattat gccaaggatg atccattgga
      841 atttaagtcc catcagtggt ttggagcatc tgtgaggtcg aaacaggata aaattttggc
      901 ctgtgcccca ttgtaccatt ggagaactga gatgaaacag gagcgagagc ctgttggaac
      961 atgctttctt caagatggaa caaagactgt tgagtatgct ccatgtagat cacgtcagct
     1021 tatttcggat caagtggcag aaatcgtatc taaatacgac cccaatgttt acagcatcaa
     1081 gtataataac caattagcaa ctcggactgc acaagctatt tttgatgaca gctatttggg
     1141 ttattctgtg gctgtcggag atttcaatgg tgatggcata gatgactttg tttcaggagt
     1201 tccaagagca gcaaggactt tgggaatggt ttatatttat gatgggaaga acatgtcctc
     1261 cttatacaat tttactggcg agcagatggc tgcatatttc ggattttctg tagctgccac
     1321 tgacattaat ggagatgatt atgcagatgt gtttattgga gcacctctct tcatggatcg
     1381 tggctctgat ggcaaactcc aagaggtggg gcaggtctca gtgtctctac agagagcttc
     1441 aggagacttc cagacgacaa agctgaatgg atttgaggtc tttgcacggt ttggcagtgc
     1501 catagctcct ttgggagatc tggaccagga tggtttcaat gatattgcaa ttgctgctcc
     1561 atatgggggt gaagataaaa aaggaattgt ttatatcttc aatggaagat caacaggctt
     1621 gaacgcagtc ccatctcaaa tccttgaagg gcagtgggct gctcgaagca tgccaccaag
     1681 ctttggctat tcaatgaaag gagccacaga tatagacaaa aatggatatc cagacttaat
     1741 tgtaggagct tttggtgtag atcgagctat cttatacagg gccagaccag ttatcactgt
     1801 aaatgctggt cttgaagtgt accctagcat tttaaatcaa gacaataaaa cctgctcact
     1861 gcctggaaca gctctcaaag tttcctgttt taatgttagg ttctgcttaa aggcagatgg
     1921 caaaggagta cttcccagga aacttaattt ccaggtggaa cttcttttgg ataaactcaa
     1981 gcaaaaggga gcaattcgac gagcactgtt tctctacagc aggtccccaa gtcactccaa
     2041 gaacatgact atttcaaggg ggggactgat gcagtgtgag gaattgatag cgtatctgcg
     2101 ggatgaatct gaatttagag acaaactcac tccaattact atttttatgg aatatcggtt
     2161 ggattataga acagctgctg atacaacagg cttgcaaccc attcttaacc agttcacgcc
     2221 tgctaacatt agtcgacagg ctcacattct acttgactgt ggtgaagaca atgtctgtaa
     2281 acccaagctg gaagtttctg tagatagtga tcaaaagaag atctatattg gggatgacaa
     2341 ccctctgaca ttgattgtta aggctcagaa tcaaggagaa ggtgcctacg aagctgagct
     2401 catcgtttcc attccactgc aggctgattt catcggggtt gtccgaaaca atgaagcctt
     2461 agcaagactt tcctgtgcat ttaagacaga aaaccaaact cgccaggtgg tatgtgacct
     2521 tggaaaccca atgaaggctg gaactcaact cttagctggt cttcgtttca gtgtgcacca
     2581 gcagtcagag atggatactt ctgtgaaatt tgacttacaa atccaaagct caaatctatt
     2641 tgacaaagta agcccagttg tatctcacaa agttgatctt gctgttttag ctgcagttga
     2701 gataagagga gtctcgagtc ctgatcatat ctttcttccg attccaaact gggagcacaa
     2761 ggagaaccct gagactgaag aagatgttgg gccagttgtt cagcacatct atgagctgag
     2821 aaacaatggt ccaagttcat tcagcaaggc aatgctccat cttcagtggc cttacaaata
     2881 taataataac actctgttgt atatccttca ttatgatatt gatggaccaa tgaactgcac
     2941 ttcagatatg gagatcaacc ctttgagaat taagatctca tctttgcaaa caactgaaaa
     3001 gaatgacacg gttgccgggc aaggtgagcg ggaccatctc atcactaagc gggatcttgc
     3061 cctcagtgaa ggagatattc acactttggg ttgtggagtt gctcagtgct tgaagattgt
     3121 ctgccaagtt gggagattag acagaggaaa gagtgcaatc ttgtacgtaa agtcattact
     3181 gtggactgag acttttatga ataaagaaaa tcagaatcat tcctattctc tgaagtcgtc
     3241 tgcttcattt aatgtcatag agtttcctta taagaatctt ccaattgagg atatcaccaa
     3301 ctccacattg gttaccacta atgtcacctg gggcattcag ccagcgccca tgcctgtgcc
     3361 tgtgtgggtg atcattttag cagttctagc aggattgttg ctactggctg ttttggtatt
     3421 tgtaatgtac aggatgggct tttttaaacg ggtccggcca cctcaagaag aacaagaaag
     3481 ggagcagctt caacctcatg aaaatggtga aggaaactca gaaacttaat actagagcca
     3541 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     3601 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     3661 tttata
//