LOCUS       BBa_K1956014            2016 bp    DNA     linear       18-May-2024
DEFINITION  Noise in Device measurement
ACCESSION   BBa_K1956014
VERSION     BBa_K1956014.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             64..76
                     /label=RBS-3\Weak
     RBS             70..73
                     /label=RBS
     misc_feature    64..76
                     /label=BBa_B0032
     RBS             64..76
                     /label=BBa_B0032
     CDS             83..802
                     /label=GFP protein
     CDS             83..802
                     /label=BBa_E0040
     stem_loop       822..865
                     /label=stem_loop
     misc_feature    811..890
                     /label=BBa_B0010
     terminator      811..890
                     /label=BBa_B0010
     stem_loop       906..925
                     /label=T7 TE
     misc_feature    932..932
                     /label=stop
     polyA_site      926..939
                     /label=polya
     misc_feature    899..939
                     /label=BBa_B0012
     terminator      899..939
                     /label=BBa_B0012
     misc_feature    83..939
                     /label=BBa_I13401
     CDS             948..1035
                     /label=end of LacI coding region (inactive)
     misc_feature    948..1147
                     /label=BBa_R0010
     misc_feature    1036..1073
                     /label=CAP binding site
     promoter        1084..1089
                     /label=-35
     promoter        1108..1113
                     /label=-10
     misc_feature    1113..1147
                     /label=LacI binding site
     misc_feature    1120..1120
                     /label=start
     promoter        948..1147
                     /label=BBa_R0010
     misc_feature    1160..1163
                     /label=conserved
     RBS             1156..1167
                     /label=BBa_B0034
     CDS             1174..1848
                     /label=mrfp1
     misc_feature    1855..1879
                     /label=Help:Barcodes
     CDS             1174..1854
                     /label=BBa_E1010
     stem_loop       1899..1942
                     /label=stem_loop
     misc_feature    1888..1967
                     /label=BBa_B0010
     terminator      1888..1967
                     /label=BBa_B0010
     stem_loop       1983..2002
                     /label=T7 TE
     misc_feature    2009..2009
                     /label=stop
     polyA_site      2003..2016
                     /label=polya
     misc_feature    1976..2016
                     /label=BBa_B0012
     terminator      1976..2016
                     /label=BBa_B0012
     misc_feature    948..2016
                     /label=BBa_J04450
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagtcacaca ggaaagtact agatgcgtaa aggagaagaa cttttcactg gagttgtccc
      121 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
      181 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
      241 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag
      301 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
      361 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
      421 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
      481 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
      541 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
      601 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
      661 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
      721 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
      781 ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt
      841 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      901 acactggctc accttcgggt gggcctttct gcgtttatat actagagcaa tacgcaaacc
      961 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     1021 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     1081 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     1141 tcacacatac tagagaaaga ggagaaatac tagatggctt cctccgaaga cgttatcaaa
     1201 gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc
     1261 gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc
     1321 aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc
     1381 aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa
     1441 ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag
     1501 gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc
     1561 ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt
     1621 atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac
     1681 ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg
     1741 ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc
     1801 atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga
     1861 tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1921 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1981 ctggctcacc ttcgggtggg cctttctgcg tttata
//