LOCUS       BBa_K195606              511 bp    DNA     linear       10-May-2024
DEFINITION  pTet with activator gene CII
ACCESSION   BBa_K195606
VERSION     BBa_K195606.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..374
                     /label=BBa_K116602
     stem_loop       394..437
                     /label=stem_loop
     misc_feature    383..462
                     /label=BBa_B0010
     terminator      383..462
                     /label=BBa_B0010
     stem_loop       478..497
                     /label=T7 TE
     misc_feature    504..504
                     /label=stop
     polyA_site      498..511
                     /label=polya
     misc_feature    471..511
                     /label=BBa_B0012
     terminator      471..511
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggttcgtg caaacaaacg caacgaggct ctacgaatcg
      121 agagtgcgtt gcttaacaaa atcgcaatgc ttggaactga gaagacagcg gaagctgtgg
      181 gcgttgataa gtcgcagatc agcaggtgga agagggactg gattccaaag ttctcaatgc
      241 tgcttgctgt tcttgaatgg ggggtcgttg acgacgacat ggctcgattg gcgcgacaag
      301 ttgctgcgat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
      361 agatggagtt ctgatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
      421 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
      481 tcaccttcgg gtgggccttt ctgcgtttat a
//