LOCUS       BBa_K195607              534 bp    DNA     linear       12-May-2024
DEFINITION  pPenI with activator gene CII
ACCESSION   BBa_K195607
VERSION     BBa_K195607.1  
FEATURES             Location/Qualifiers
     misc_feature    1..77
                     /label=PenI
     promoter        23..28
                     /label=-35
     promoter        46..51
                     /label=-10
     misc_feature    31..53
                     /label=dimer left half
     misc_feature    55..76
                     /label=dimer right half
     promoter        1..77
                     /label=BBa_R0074
     misc_feature    90..93
                     /label=conserved
     RBS             86..97
                     /label=BBa_B0034
     CDS             104..397
                     /label=BBa_K116602
     stem_loop       417..460
                     /label=stem_loop
     misc_feature    406..485
                     /label=BBa_B0010
     terminator      406..485
                     /label=BBa_B0010
     stem_loop       501..520
                     /label=T7 TE
     misc_feature    527..527
                     /label=stop
     polyA_site      521..534
                     /label=polya
     misc_feature    494..534
                     /label=BBa_B0012
     terminator      494..534
                     /label=BBa_B0012
ORIGIN
        1 tcatttccaa ccgaaaaaag gcttgcattt aaatcttaca tatgtaatac tttcaaagac
       61 tacatttgta agatttgtac tagagaaaga ggagaaatac tagatggttc gtgcaaacaa
      121 acgcaacgag gctctacgaa tcgagagtgc gttgcttaac aaaatcgcaa tgcttggaac
      181 tgagaagaca gcggaagctg tgggcgttga taagtcgcag atcagcaggt ggaagaggga
      241 ctggattcca aagttctcaa tgctgcttgc tgttcttgaa tggggggtcg ttgacgacga
      301 catggctcga ttggcgcgac aagttgctgc gattctcacc aataaaaaac gcccggcggc
      361 aaccgagcgt tctgaacaaa tccagatgga gttctgatac tagagccagg catcaaataa
      421 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      481 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//