LOCUS       BBa_K195608              657 bp    DNA     linear       10-May-2024
DEFINITION  pLac with activator gene CII
ACCESSION   BBa_K195608
VERSION     BBa_K195608.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..520
                     /label=BBa_K116602
     stem_loop       540..583
                     /label=stem_loop
     misc_feature    529..608
                     /label=BBa_B0010
     terminator      529..608
                     /label=BBa_B0010
     stem_loop       624..643
                     /label=T7 TE
     misc_feature    650..650
                     /label=stop
     polyA_site      644..657
                     /label=polya
     misc_feature    617..657
                     /label=BBa_B0012
     terminator      617..657
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ttcgtgcaaa
      241 caaacgcaac gaggctctac gaatcgagag tgcgttgctt aacaaaatcg caatgcttgg
      301 aactgagaag acagcggaag ctgtgggcgt tgataagtcg cagatcagca ggtggaagag
      361 ggactggatt ccaaagttct caatgctgct tgctgttctt gaatgggggg tcgttgacga
      421 cgacatggct cgattggcgc gacaagttgc tgcgattctc accaataaaa aacgcccggc
      481 ggcaaccgag cgttctgaac aaatccagat ggagttctga tactagagcc aggcatcaaa
      541 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      601 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//