LOCUS BBa_K1960002 3548 bp DNA linear 21-May-2024 DEFINITION Toggle switch controlled by sensing device ACCESSION BBa_K1960002 VERSION BBa_K1960002.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..1104 /label=BBa_I732100 stem_loop 1124..1167 /label=stem_loop misc_feature 1113..1192 /label=BBa_B0010 terminator 1113..1192 /label=BBa_B0010 stem_loop 1208..1227 /label=T7 TE misc_feature 1234..1234 /label=stop polyA_site 1228..1241 /label=polya misc_feature 1201..1241 /label=BBa_B0012 terminator 1201..1241 /label=BBa_B0012 misc_feature 1..1241 /label=BBa_I732820 misc_feature 1252..1268 /label=lac O1 promoter 1269..1274 /label=-35 misc_feature 1275..1291 /label=lac O1 promoter 1292..1297 /label=-10 misc_feature 1250..1303 /label=BBa_R0011 promoter 1250..1303 /label=BBa_R0011 misc_feature 1317..1320 /label=conserved RBS 1313..1324 /label=BBa_B0034 CDS 1334..2041 /label=cI lambda misc_feature 2042..2074 /label=LVA misc_feature 2081..2105 /label=Help:Barcodes CDS 1331..2080 /label=BBa_C0051 stem_loop 2125..2168 /label=stem_loop misc_feature 2114..2193 /label=BBa_B0010 terminator 2114..2193 /label=BBa_B0010 stem_loop 2209..2228 /label=T7 TE misc_feature 2235..2235 /label=stop polyA_site 2229..2242 /label=polya misc_feature 2202..2242 /label=BBa_B0012 terminator 2202..2242 /label=BBa_B0012 misc_feature 1313..2242 /label=BBa_P0451 promoter 2288..2293 /label=-10 promoter 2265..2270 /label=-35 misc_feature 2275..2291 /label=OR1 misc_feature 2251..2267 /label=OR2 misc_feature 2251..2299 /label=BBa_R0051 promoter 2251..2299 /label=BBa_R0051 misc_feature 2312..2315 /label=conserved RBS 2308..2319 /label=BBa_B0034 CDS 2326..3411 /label=BBa_I732100 stem_loop 3431..3474 /label=stem_loop misc_feature 3420..3499 /label=BBa_B0010 terminator 3420..3499 /label=BBa_B0010 stem_loop 3515..3534 /label=T7 TE misc_feature 3541..3541 /label=stop polyA_site 3535..3548 /label=polya misc_feature 3508..3548 /label=BBa_B0012 terminator 3508..3548 /label=BBa_B0012 misc_feature 2308..3548 /label=BBa_I732820 ORIGIN 1 aaagaggaga aatactagat gaaaccagta acgttatacg atgtcgcaga gtatgccggt 61 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg 121 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa 181 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac 241 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg 301 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt 361 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt 421 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca 481 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg 541 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg 601 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg 661 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat 721 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg 781 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac 841 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc 901 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag 961 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg 1021 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 1081 cgactggaaa gcgggcagta ataatactag agccaggcat caaataaaac gaaaggctca 1141 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 1201 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga attgtgagcg 1261 gataacaatt gacattgtga gcggataaca agatactgag cacatactag agaaagagga 1321 gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg aggacgcacg 1381 tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc aggaatctgt 1441 cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg gcatcaatgc 1501 attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg ttgaagaatt 1561 tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta tgcagccgtc 1621 acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga tgttctcacc 1681 tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa ccaaaaaagc 1741 cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac caacaggctc 1801 caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg ctgttgagcc 1861 aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga aactgatcag 1921 ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga tcccatgcaa 1981 tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag agacgtttgg 2041 cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt gctagtgtag 2101 atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 2161 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 2221 ggtgggcctt tctgcgttta tatactagag taacaccgtg cgtgttgact attttacctc 2281 tggcggtgat aatggttgct actagagaaa gaggagaaat actagatgaa accagtaacg 2341 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac 2401 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg 2461 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc 2521 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct 2581 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa 2641 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac 2701 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg 2761 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac 2821 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta 2881 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc 2941 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt 3001 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc 3061 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt 3121 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg 3181 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg 3241 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa 3301 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 3361 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtaata atactagagc 3421 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 3481 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 3541 cgtttata //