LOCUS       BBa_K1960003            1791 bp    DNA     linear       18-May-2024
DEFINITION  Quorum sensing controller for gram-negative bacterium with cyan 
            fluorescent protein (CFP)
ACCESSION   BBa_K1960003
VERSION     BBa_K1960003.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..63
                     /label=prefix
     misc_feature    235..235
                     /label=T
     misc_feature    553..553
                     /label=A
     CDS             62..811
                     /label=luxR
     misc_feature    62..817
                     /label=BBa_C0062
     misc_feature    818..842
                     /label=Help:Barcodes
     CDS             62..817
                     /label=BBa_C0062
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     terminator      851..979
                     /label=BBa_B0015
     misc_feature    988..1007
                     /label=LuxR/HSL
     promoter        1007..1012
                     /label=-35
     promoter        1029..1034
                     /label=-10
     misc_feature    1040..1040
                     /label=start
     misc_feature    988..1042
                     /label=BBa_R0062
     promoter        988..1042
                     /label=BBa_R0062
     misc_feature    1..1042
                     /label=BBa_K1960004
     misc_feature    1055..1058
                     /label=conserved
     RBS             1051..1062
                     /label=BBa_B0034
     CDS             1069..1791
                     /label=BBa_E0020
     misc_feature    1051..1791
                     /label=BBa_S01022
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag
      121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata
      181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga
      241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc
      301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa
      361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac
      421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca
      481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt
      541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa
      601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc
      661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa
      721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac
      781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc
      841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa
     1021 tggtttgtta tagtcgaata aatactagag aaagaggaga aatactagat ggtgagcaag
     1081 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtgaac
     1141 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1201 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1261 ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1321 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1381 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1441 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1501 aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
     1561 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1621 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1681 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1741 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaata a
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