LOCUS       BBa_K1960400            1987 bp    DNA     linear       20-May-2024
DEFINITION  GFP and lacI
ACCESSION   BBa_K1960400
VERSION     BBa_K1960400.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..738
                     /label=GFP protein
     CDS             19..738
                     /label=BBa_E0040
     misc_feature    1..738
                     /label=BBa_I13500
     misc_feature    751..754
                     /label=conserved
     RBS             747..758
                     /label=BBa_B0034
     CDS             765..1850
                     /label=BBa_I732100
     stem_loop       1870..1913
                     /label=stem_loop
     misc_feature    1859..1938
                     /label=BBa_B0010
     terminator      1859..1938
                     /label=BBa_B0010
     stem_loop       1954..1973
                     /label=T7 TE
     misc_feature    1980..1980
                     /label=stop
     polyA_site      1974..1987
                     /label=polya
     misc_feature    1947..1987
                     /label=BBa_B0012
     terminator      1947..1987
                     /label=BBa_B0012
     misc_feature    747..1987
                     /label=BBa_I732820
ORIGIN
        1 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
       61 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      121 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      181 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      241 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      301 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      361 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      421 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      481 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      541 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      601 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      661 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      721 gaactataca aataataata ctagagaaag aggagaaata ctagatgaaa ccagtaacgt
      781 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
      841 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
      901 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
      961 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
     1021 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag
     1081 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact
     1141 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt
     1201 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg
     1261 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag
     1321 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
     1381 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt
     1441 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca
     1501 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg
     1561 cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt
     1621 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc
     1681 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
     1741 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     1801 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtaataa tactagagcc
     1861 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1921 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1981 gtttata
//