LOCUS       BBa_K1962010            1721 bp    DNA     linear       13-Jun-2024
DEFINITION  Bile Salts Sensing Device
ACCESSION   BBa_K1962010
VERSION     BBa_K1962010.1  
FEATURES             Location/Qualifiers
     promoter        1..835
                     /label=BBa_K318514
     RBS             844..858
                     /label=RBS-1\Strong
     RBS             851..855
                     /label=RBS
     misc_feature    844..858
                     /label=BBa_B0030
     RBS             844..858
                     /label=BBa_B0030
     CDS             865..1584
                     /label=GFP protein
     CDS             865..1584
                     /label=BBa_E0040
     stem_loop       1604..1647
                     /label=stem_loop
     misc_feature    1593..1672
                     /label=BBa_B0010
     terminator      1593..1672
                     /label=BBa_B0010
     stem_loop       1688..1707
                     /label=T7 TE
     misc_feature    1714..1714
                     /label=stop
     polyA_site      1708..1721
                     /label=polya
     misc_feature    1681..1721
                     /label=BBa_B0012
     terminator      1681..1721
                     /label=BBa_B0012
     misc_feature    844..1721
                     /label=BBa_E0840
ORIGIN
        1 ttatcagggg gagccgttga ccgtcgacgc gcgcagcgtc ggacacaatt gatacatctc
       61 cagcaggatc gtgacgtagg cgcgagcttc tttttttaaa tcaaacgatt gcggagcaaa
      121 taaccagttt tccatgagcc ctgaaataaa gctgcgcatc agtatcgccg cacgacgggt
      181 cagcagattt tcaggcagca ttttagcatt aatacaatgt tttaacgttt gttcaatccg
      241 atcgtaactt tccagacaaa ggctacgttg cgcctgttga accacaacca tttctccgac
      301 aaactcacat ttatggaata taatctccat caataagcgg cgtcgttctt ctgttacagt
      361 agcttcaaga atatgaacta aaatttctct taatacagat agtggatcgt cggggaattt
      421 tgcctgatac tcaatctcaa gctcaccaat attggattct gatagctccc agatctcact
      481 gaataaatcc gacttatttt tgaaatgcca atagattgcg ccgcgcgtca cgccagcagc
      541 gttcgcaatc tccgccagcg aggttgccga tacgccttgc tgcgagaaca aacgcagggc
      601 cacatccagg atgtgttgtc gtgtctccag cgcttgttgt ttggtttttc gtgccatatg
      661 tcggtgaatt tacaggcgtt agatttacat acatttatgg atgtatgtac catagcacga
      721 cgataatata aacgcagcaa tgggtttaag gacctttgac cattgaccaa tttgaaatcg
      781 gacactcgag gtttacatat gaacaaaaac agagggttaa cgcctctggc ggtcgtacta
      841 gagattaaag aggagaaata ctagatgcgt aaaggagaag aacttttcac tggagttgtc
      901 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
      961 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
     1021 ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg
     1081 agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat
     1141 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc
     1201 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa
     1261 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc
     1321 atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa
     1381 gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct
     1441 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac
     1501 gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc
     1561 atggatgaac tatacaaata ataatactag agccaggcat caaataaaac gaaaggctca
     1621 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1681 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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