BBa_K1962011 1 BBa_K1962011 A device for expression of Im-Ia in response to bile salts 2016-10-11T11:00:00Z 2016-10-12T01:02:06Z Existing and new biobricks. This is a composite part which consists of a bile salt responsive promoter (<partinfo>BBa_K318514</partinfo>), a RBS (<partinfo>BBa_B0030</partinfo>) and the biobrick encoding the Immunity Protein to Colicin Ia (<partinfo>BBa_K1962001</partinfo>). For best results use in conjunction with the RamA transcriptional activator (<partinfo>BBa_K1962009</partinfo>). The promoter activity has been chracterised using a GFP reporter (<partinfo>BBa_K1962010</partinfo>). false false _2429_ 8083 8083 9 false REC[10] compliant. false Frank Sargent component2493439 1 BBa_K318514 component2493444 1 BBa_K1962001 component2493441 1 BBa_B0030 annotation2493439 1 BBa_K318514 range2493439 1 1 835 annotation2493441 1 BBa_B0030 range2493441 1 844 858 annotation2493444 1 BBa_K1962001 range2493444 1 865 1197 BBa_K318514 1 BBa_K318514 acrRA 2010-09-25T11:00:00Z 2015-05-08T01:11:58Z uc uc false false _438_ 0 4504 9 Not in stock false uc false Sarah R. Sandock BBa_K1962001 1 BBa_K1962001 Immunity Protein Im-Ia 2016-10-10T11:00:00Z 2016-10-11T03:07:20Z This part was designed by back-translation of the primary amino acid sequence into DNA sequence, followed by optimisiation for expression in an E. coli chassis. The biobrick was then synthesised de novo by IDT. This biobrick codes for the Immunity Protein for Colicin Ia (<partinfo>BBa_K1962000</partinfo>). Colicin Ia is a channel-forming bacteriocin that depolarizes the cytoplasmic inner membrane of target bacteria, leading to dissipation of cellular energy. This Immunity Protein is tightly linked to its specific Colicin bacteriocin domain to protect the colicinogenic cell from the cytotoxic activity of the colicin. false false _2429_ 8083 8083 9 false Compliant with RFC[10]. false Frank Sargent annotation2491784 1 Immunity Protein Im-Ia range2491784 1 1 333 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1702 1 RBS range1702 1 8 12 annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1701 1 RBS-1\Strong range1701 1 1 15 BBa_K318514_sequence 1 ttatcagggggagccgttgaccgtcgacgcgcgcagcgtcggacacaattgatacatctccagcaggatcgtgacgtaggcgcgagcttctttttttaaatcaaacgattgcggagcaaataaccagttttccatgagccctgaaataaagctgcgcatcagtatcgccgcacgacgggtcagcagattttcaggcagcattttagcattaatacaatgttttaacgtttgttcaatccgatcgtaactttccagacaaaggctacgttgcgcctgttgaaccacaaccatttctccgacaaactcacatttatggaatataatctccatcaataagcggcgtcgttcttctgttacagtagcttcaagaatatgaactaaaatttctcttaatacagatagtggatcgtcggggaattttgcctgatactcaatctcaagctcaccaatattggattctgatagctcccagatctcactgaataaatccgacttatttttgaaatgccaatagattgcgccgcgcgtcacgccagcagcgttcgcaatctccgccagcgaggttgccgatacgccttgctgcgagaacaaacgcagggccacatccaggatgtgttgtcgtgtctccagcgcttgttgtttggtttttcgtgccatatgtcggtgaatttacaggcgttagatttacatacatttatggatgtatgtaccatagcacgacgataatataaacgcagcaatgggtttaaggacctttgaccattgaccaatttgaaatcggacactcgaggtttacatatgaacaaaaacagagggttaacgcctctggcggtcg BBa_K1962011_sequence 1 ttatcagggggagccgttgaccgtcgacgcgcgcagcgtcggacacaattgatacatctccagcaggatcgtgacgtaggcgcgagcttctttttttaaatcaaacgattgcggagcaaataaccagttttccatgagccctgaaataaagctgcgcatcagtatcgccgcacgacgggtcagcagattttcaggcagcattttagcattaatacaatgttttaacgtttgttcaatccgatcgtaactttccagacaaaggctacgttgcgcctgttgaaccacaaccatttctccgacaaactcacatttatggaatataatctccatcaataagcggcgtcgttcttctgttacagtagcttcaagaatatgaactaaaatttctcttaatacagatagtggatcgtcggggaattttgcctgatactcaatctcaagctcaccaatattggattctgatagctcccagatctcactgaataaatccgacttatttttgaaatgccaatagattgcgccgcgcgtcacgccagcagcgttcgcaatctccgccagcgaggttgccgatacgccttgctgcgagaacaaacgcagggccacatccaggatgtgttgtcgtgtctccagcgcttgttgtttggtttttcgtgccatatgtcggtgaatttacaggcgttagatttacatacatttatggatgtatgtaccatagcacgacgataatataaacgcagcaatgggtttaaggacctttgaccattgaccaatttgaaatcggacactcgaggtttacatatgaacaaaaacagagggttaacgcctctggcggtcgtactagagattaaagaggagaaatactagatgaaccgtaaatactacttcaacaacatgtggtggggttgggttaccggtggttacatgctgtacatgtcttgggactacgagttcaaataccgtctgctgttctggtgcatctctctgtgcggtatggttctgtacccggttgcgaaatggtacatcgaagacaccgcgctgaaattcacccgtccggacttctggaactctggtttcttcgcggacaccccgggtaaaatgggtctgctggcggtttacaccggtaccgttttcatcctgtctctgccgctgtctatgatctacatcctgtctgttatcatcaaacgtctgtctgttcgt BBa_B0030_sequence 1 attaaagaggagaaa BBa_K1962001_sequence 1 atgaaccgtaaatactacttcaacaacatgtggtggggttgggttaccggtggttacatgctgtacatgtcttgggactacgagttcaaataccgtctgctgttctggtgcatctctctgtgcggtatggttctgtacccggttgcgaaatggtacatcgaagacaccgcgctgaaattcacccgtccggacttctggaactctggtttcttcgcggacaccccgggtaaaatgggtctgctggcggtttacaccggtaccgttttcatcctgtctctgccgctgtctatgatctacatcctgtctgttatcatcaaacgtctgtctgttcgt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z