LOCUS       BBa_K1962013            1150 bp    DNA     linear       13-Jun-2024
DEFINITION  A pH Sensing Device Based on The GadA Promoter
ACCESSION   BBa_K1962013
VERSION     BBa_K1962013.1  
FEATURES             Location/Qualifiers
     promoter        1..264
                     /label=BBa_K1231001
     RBS             273..287
                     /label=RBS-1\Strong
     RBS             280..284
                     /label=RBS
     misc_feature    273..287
                     /label=BBa_B0030
     RBS             273..287
                     /label=BBa_B0030
     CDS             294..1013
                     /label=GFP protein
     CDS             294..1013
                     /label=BBa_E0040
     stem_loop       1033..1076
                     /label=stem_loop
     misc_feature    1022..1101
                     /label=BBa_B0010
     terminator      1022..1101
                     /label=BBa_B0010
     stem_loop       1117..1136
                     /label=T7 TE
     misc_feature    1143..1143
                     /label=stop
     polyA_site      1137..1150
                     /label=polya
     misc_feature    1110..1150
                     /label=BBa_B0012
     terminator      1110..1150
                     /label=BBa_B0012
     misc_feature    273..1150
                     /label=BBa_E0840
ORIGIN
        1 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
       61 tttgatcgcc cgaacagcaa tgtttgggcg atttttatta cgataataaa gtctgttttt
      121 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
      181 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
      241 cttcaaataa atttaaggag ttcgtactag agattaaaga ggagaaatac tagatgcgta
      301 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
      361 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
      421 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
      481 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact
      541 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
      601 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
      661 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
      721 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
      781 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
      841 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
      901 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt
      961 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa taatactaga
     1021 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1081 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1141 tgcgtttata
//