BBa_K1963009 1 BBa_K1963009 Device for rhamnose-dependent expression of an OsmY fusion to Serratia Hfq. 2016-10-08T11:00:00Z 2016-10-09T03:24:55Z Based on natural sequences. A rhamnose-regulated device based on existing and new (2016) biobricks. This is designed to induce production of a fusion protein between E. coli OsmY (a secerted protein) and Serratia marcescens Hfq (an RNA binding protein). Co-expression with Serratia sRNA may generate a specific RNA secretion system. false false _2430_ 8083 8083 9 false RFC[10] compliant. false Frank Sargent component2488815 1 BBa_K914003 component2488821 1 BBa_K1963006 component2488817 1 BBa_B0030 annotation2488815 1 BBa_K914003 range2488815 1 1 122 annotation2488821 1 BBa_K1963006 range2488821 1 152 1060 annotation2488817 1 BBa_B0030 range2488817 1 131 145 BBa_K1963006 1 BBa_K1963006 A translational fusion between E. coli OsmY and Serratia Hfq 2016-10-08T11:00:00Z 2016-10-09T02:58:44Z Genomic sequence amplified by PCR. The E. coli OsmY protein is secreted from the cell. Serratia Hfq is an RNA binding protein. This translational fusion between the two may allow Hfq to be secreted from an E. coli chassis organism, together with any specific sRNA that may be attached. Pairing Serratia Hfq and Serratia sRNA sequences may afford some specificity to the system. false false _2430_ 8083 8083 9 false Compliant with RFC[10] standard. false Frank Sargent annotation2488756 1 Serratia Hfq range2488756 1 604 909 annotation2488754 1 OsmY range2488754 1 1 603 BBa_K914003 1 BBa_K914003 L-rhamnose-inducible promoter (pRha) 2012-09-19T11:00:00Z 2015-05-08T01:13:45Z Amplification of the plasmid pJOE3075 (Dr. Altenbuchner). Released HQ 2013 L-rhamnose-inducible promoter is capable of high-level recombinant protein expression in the presence of L-rhamnose, it is also tightly regulated in the absence of L-rhamnose by the addition of D-glucose. false false _1179_ 0 13487 9 In stock true One base pair modified (90: A -> T) to avoid EcoRI site. Mutation made in a less conserved base pair. false Denis Samuylov annotation2188559 1 pRha range2188559 1 1 122 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1702 1 RBS range1702 1 8 12 annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1701 1 RBS-1\Strong range1701 1 1 15 BBa_K1963006_sequence 1 atgactatgacaagactgaagatttcgaaaactctgctggctgtaatgttgacctctgccgtcgcgaccggctctgcctacgcggaaaacaacgcgcagactaccaatgaaagcgcagggcaaaaagtcgatagctctatgaataaagtcggtaatttcatggatgacagcgccatcaccgcgaaagtgaaggcggccctggtggatcatgacaacatcaagagcaccgatatctctgtaaaaaccgatcaaaaagtcgtgaccctgagcggtttcgttgaaagccaggcccaggccgaagaggcagtgaaagtggcgaaaggcgttgaaggggtgacctctgtcagcgacaaactgcacgttcgcgacgctaaagaaggctcggtgaagggctacgcgggtgacaccgccaccaccagtgaaatcaaagccaaactgctggcggacgatatcgtcccttcccgtcatgtgaaagttgaaaccaccgacggcgtggttcagctctccggtaccgtcgattctcaggcacaaagtgaccgtgctgaaagtatcgccaaagcggtagatggtgtgaaaagcgttaaaaatgatctgaaaactaagatggctaaggggcaatctttgcaagatccgttcctgaacgcattgcgtcgtgaacgtgttccggtttctatttatttggtgaatggtattaagctgcaaggccagattgagtcttttgaccagtttgtcatcctgttgaagaacacggttagccagatggtgtacaagcacgctatctctaccgttgtcccgtcacgcccggtttcgcatcacagcaacaacccgagcggcggttcaagcaactatcaccatggcaacaatccgtctgcgcagcaacagccgcagcaggaaagcgatgacgctgaa BBa_B0030_sequence 1 attaaagaggagaaa BBa_K1963009_sequence 1 ccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaatactagagattaaagaggagaaatactagatgactatgacaagactgaagatttcgaaaactctgctggctgtaatgttgacctctgccgtcgcgaccggctctgcctacgcggaaaacaacgcgcagactaccaatgaaagcgcagggcaaaaagtcgatagctctatgaataaagtcggtaatttcatggatgacagcgccatcaccgcgaaagtgaaggcggccctggtggatcatgacaacatcaagagcaccgatatctctgtaaaaaccgatcaaaaagtcgtgaccctgagcggtttcgttgaaagccaggcccaggccgaagaggcagtgaaagtggcgaaaggcgttgaaggggtgacctctgtcagcgacaaactgcacgttcgcgacgctaaagaaggctcggtgaagggctacgcgggtgacaccgccaccaccagtgaaatcaaagccaaactgctggcggacgatatcgtcccttcccgtcatgtgaaagttgaaaccaccgacggcgtggttcagctctccggtaccgtcgattctcaggcacaaagtgaccgtgctgaaagtatcgccaaagcggtagatggtgtgaaaagcgttaaaaatgatctgaaaactaagatggctaaggggcaatctttgcaagatccgttcctgaacgcattgcgtcgtgaacgtgttccggtttctatttatttggtgaatggtattaagctgcaaggccagattgagtcttttgaccagtttgtcatcctgttgaagaacacggttagccagatggtgtacaagcacgctatctctaccgttgtcccgtcacgcccggtttcgcatcacagcaacaacccgagcggcggttcaagcaactatcaccatggcaacaatccgtctgcgcagcaacagccgcagcaggaaagcgatgacgctgaa BBa_K914003_sequence 1 ccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z