LOCUS BBa_K1972014 7367 bp DNA linear 01-Jun-2024 DEFINITION dszBBAC with tac promoter and dszD with lac promotor induced by IPTG ACCESSION BBa_K1972014 VERSION BBa_K1972014.1 FEATURES Location/Qualifiers promoter 1..53 /label=tac promoter RBS 54..72 /label=RBS CDS 73..1170 /label=dszB-optimized CDS 1..1170 /label=BBa_K1972005 stem_loop 1190..1233 /label=stem_loop misc_feature 1179..1258 /label=BBa_B0010 terminator 1179..1258 /label=BBa_B0010 stem_loop 1274..1293 /label=T7 TE misc_feature 1300..1300 /label=stop polyA_site 1294..1307 /label=polya misc_feature 1267..1307 /label=BBa_B0012 terminator 1267..1307 /label=BBa_B0012 terminator 1179..1307 /label=BBa_B0015 CDS 1..1307 /label=BBa_K1972008 promoter 1316..1368 /label=tac promoter RBS 1369..1387 /label=RBS CDS 1388..2485 /label=dszB-optimized CDS 1316..3085 /label=BBa_K1972005 misc_feature 1316..3085 /label=BBa_K1972005 misc_feature 3094..3876 /label=BBa_K1972004 misc_feature 3885..5156 /label= BBa_K1972006 promoter 5294..5336 /label=LacUV5 promoter misc_feature 5165..5293 /label=double teminator misc_feature 5337..5932 /label=BBa_K1972003 misc_feature 5933..6061 /label=double teminator promoter 6070..6147 /label=LacI promoter CDS 6271..7234 /label=LacI misc_feature 7239..7367 /label=double teminator CDS 1316..7367 /label=BBa_K1972013 ORIGIN 1 ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa caaaactgca 61 aggacaacct tcatgacctc tcgcgttgat ccggccaatc cgggtagcga actggatagt 121 gccattcgcg ataccttaac ctatagtaat tgtccggttc cgaatgcact gttaaccgcc 181 tctgaaagcg gctttctgga tgcagcaggt attgaactgg atgtgctgag cggccagcag 241 ggtaccgttc attttaccta tgatcagccg gcatataccc gctttggcgg tgaaattccg 301 ccgctgctga gtgaaggcct gcgtgcccct ggtcgtaccc gcttactggg tattaccccg 361 ttactgggtc gccagggctt cttcgtgcgc gatgatagtc cgattaccgc agccgcagac 421 ctcgcaggtc gtcgtattgg tgtgagcgca tcagccattc gtattctgcg cggtcagctc 481 ggcgactatc tggaattaga tccgtggcgt cagaccttag ttgcattagg ctcatgggaa 541 gcacgtgcct tattacatac cctggaacat ggcgaattag gcgttgatga tgttgaactg 601 gttccgattt cttcaccggg tgtggatgtt ccggccgaac agttagaaga aagtgcaacc 661 gttaaaggtg ccgatctgtt tccggatgtt gcacgcggtc aggcagccgt gctggcaagt 721 ggcgatgtgg atgccctgta ttcttggctg ccgtgggcag gcgaattaca ggcaaccggc 781 gcacgtccgg ttgtggacct cggtttagat gaacgtaatg catatgcaag tgtttggacc 841 gtgtctagcg gtttagttcg ccagagacct ggcctggttc agcgtctggt tgatgccgcc 901 gttgatgcag gcttatgggc acgcgatcat tcagatgccg tgacctcact gcatgccgca 961 aatctgggtg tgtctaccgg cgcagttggt cagggctttg gcgcagattt tcagcagcgt 1021 ctggttccgc gtttagatca tgatgcctta gccctgttag aacgcaccca gcagtttctg 1081 ctgaccaaca acctgttaca ggaaccggtg gccttagatc agtgggccgc cccggaattt 1141 ttaaacaaca gcttaaatcg tcatcgctaa tactagagcc aggcatcaaa taaaacgaaa 1201 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 1261 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagttgac 1321 aattaatcat cggctcgtat aatgtgtgga attgtgagcg gataacaaaa ctgcaaggac 1381 aaccttcatg acctctcgcg ttgatccggc caatccgggt agcgaactgg atagtgccat 1441 tcgcgatacc ttaacctata gtaattgtcc ggttccgaat gcactgttaa ccgcctctga 1501 aagcggcttt ctggatgcag caggtattga actggatgtg ctgagcggcc agcagggtac 1561 cgttcatttt acctatgatc agccggcata tacccgcttt ggcggtgaaa ttccgccgct 1621 gctgagtgaa ggcctgcgtg cccctggtcg tacccgctta ctgggtatta ccccgttact 1681 gggtcgccag ggcttcttcg tgcgcgatga tagtccgatt accgcagccg cagacctcgc 1741 aggtcgtcgt attggtgtga gcgcatcagc cattcgtatt ctgcgcggtc agctcggcga 1801 ctatctggaa ttagatccgt ggcgtcagac cttagttgca ttaggctcat gggaagcacg 1861 tgccttatta cataccctgg aacatggcga attaggcgtt gatgatgttg aactggttcc 1921 gatttcttca ccgggtgtgg atgttccggc cgaacagtta gaagaaagtg caaccgttaa 1981 aggtgccgat ctgtttccgg atgttgcacg cggtcaggca gccgtgctgg caagtggcga 2041 tgtggatgcc ctgtattctt ggctgccgtg ggcaggcgaa ttacaggcaa ccggcgcacg 2101 tccggttgtg gacctcggtt tagatgaacg taatgcatat gcaagtgttt ggaccgtgtc 2161 tagcggttta gttcgccaga gacctggcct ggttcagcgt ctggttgatg ccgccgttga 2221 tgcaggctta tgggcacgcg atcattcaga tgccgtgacc tcactgcatg ccgcaaatct 2281 gggtgtgtct accggcgcag ttggtcaggg ctttggcgca gattttcagc agcgtctggt 2341 tccgcgttta gatcatgatg ccttagccct gttagaacgc acccagcagt ttctgctgac 2401 caacaacctg ttacaggaac cggtggcctt agatcagtgg gccgccccgg aatttttaaa 2461 caacagctta aatcgtcatc gctaatacta gagcaactgc aaggacaacc ttcaatgacc 2521 cagcagcgcc agatgcacct tgcaggcttt ttcagcgcag gcaatgtgac ccatgcacat 2581 ggtgcatggc gtcataccga tgcctctaat gattttctga gcggtaaata ttatcagcat 2641 attgcacgta ccttagaacg cggcaaattt gatctgctgt ttctgccgga tggtttagcc 2701 gttgaagata gctatggaga caatctggat accggcgtgg gcttaggtgg tcagggtgca 2761 gttgccttag aaccggcaag cgttgtggca accatggccg cagtgaccga acacctgggt 2821 ttaggtgcaa ccattagcgc cacctattat ccgccgtatc atgtggcacg tgtgtttgcc 2881 accttagatc agctgagcgg cggtcgtgtg tcttggaatg ttgtgacctc actgaatgat 2941 gcagaagcac gtaattttgg tattaatcag catctggaac atgatgcacg ctatgatcgc 3001 gccgatgaat ttctggaagc cgtgaaaaaa ctgtggaact cttgggatga agatgccctg 3061 gtgctggata aagcagccgg cgtgtttgcc gatccggcca aagttcatta tgttgatcat 3121 catggcgaat ggttaaatgt gcgcggcccg ttacaggttc cgcgtagtcc gcagggcgaa 3181 ccggttattt tacaggcagg cctgtctccg cgcggtcgtc gctttgccgg taaatgggca 3241 gaagcagtgt tttcactggc cccgaatctg gaagttatgc aggcaaccta tcagggtatt 3301 aaagcagaag ttgatgccgc aggtcgcgat ccggatcaga ccaaaatttt taccgccgtt 3361 atgccggtgc tgggcgaaag tcaggcagtg gcccaggaac gcttagaata tttgaactca 3421 ctggttcatc cggaagttgg cctgtctacc ctgagtagtc ataccggtat taatctggca 3481 gcctatccgt tagatacccc gattaaagat attctgcgcg acttgcagga tcgtaatgtt 3541 ccgacccagc tgcatatgtt tgcagccgcc acccatagtg aagaactgac cttagccgaa 3601 atgggtcgtc gctatggtac caatgtgggc tttgttccgc agtgggccgg taccggcgaa 3661 cagattgccg atgaactgat tcgtcatttt gaaggcggcg ccgcagatgg ctttattatt 3721 tcaccggcct ttctgccggg tagctatgat gaatttgtgg atcaggttgt tccggtgtta 3781 caggatcgtg gctattttcg caccgaatat cagggtaata ccctgcgtga tcatctgggc 3841 ttacgcgttc cgcagttaca gggtcagccg agctaatact agagactgca aggacaacct 3901 tcatgaccct gtctccggaa aaacagcatg ttcgtccgcg tgatgccgca gataatgatc 3961 cggttgccgt tgcacgtggc ctggcagaaa aatggcgcgc caccgccgtt gaacgtgatc 4021 gtgcaggcgg ttctgccacc gcagaacgtg aagatctgcg cgcctcaggt ctgctgtcac 4081 tgctggttcc gcgtgaatat ggcggctggg gtgcagattg gccgaccgca attgaagttg 4141 ttcgcgaaat tgcagccgca gatggctcac tgggtcatct gtttggctat catctgacca 4201 atgccccgat gattgaactg attggttcac aggaacagga agaacatctg tatacccaga 4261 ttgcacagaa caattggtgg accggtaatg catcaagcga aaataactct catgtgctgg 4321 attggaaagt tcgcgcaacc ccgaccgaag atggcggcta tgtgctgaat ggtaccaaac 4381 atttttgtag cggcgccaaa ggtagcgact tgctgtttgt gtttggcgtt gttcaggatg 4441 atagtccgca gcagggcgca attattgccg cagcaattcc gacctctcgc gccggtgtga 4501 ccccgaatga tgattgggca gccattggca tgcgtcagac cgatagcggc tctaccgatt 4561 ttcataatgt taaagtggaa ccggatgaag tgctgggtgc accgaatgcc tttgtgttag 4621 cctttattca gtcagaacgc ggtagtctgt ttgccccgat tgcccagtta atttttgcca 4681 atgtgtatct aggtattgca catggtgcat tagatgcagc acgcgaatat acccgtaccc 4741 aggcacgtcc gtggacccct gccggtattc agcaggccac cgaagatccg tataccattc 4801 gcagctacgg tgagtttacc attgcattac agggcgccga tgcagccgca cgcgaagcag 4861 cccacctctt acagaccgtg tgggataaag gcgatgccct gaccccggaa gatcgtggtg 4921 aactgatggt taaagtgagc ggcgttaaag ccttagcaac caatgccgcc ttaaatattt 4981 ctagtggtgt gtttgaagtt attggtgcac gcggcaccca tccgcgctat ggctttgatc 5041 gcttttggcg taatgttcgt acccatagct tacatgatcc ggtgagctac aagattgccg 5101 atgtgggcaa acatacctta aatggtcagt atccgattcc gggctttacc tcttaatact 5161 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 5221 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 5281 ttctgcgttt atagcttccg gctcgtataa tgtgtggaat tgtgagcgga taacaacagc 5341 cggagagttc ccgatgtctg acaagccgaa tgccgtttcc agccacacca cccccgacgt 5401 ccccgaagta gcggcgacgc ccgagttgtc caccggcatc tgcgccggtg actaccgcgc 5461 tgcgcttcgc cgccaccccg ccggtgtcac cgtcgtgacc ctcgattcgg gtaccggccc 5521 ggtgggtttc accgccacct cgttctcgtc cgtctccctc gagccgccgc tcgtctcgtt 5581 caacatcgcg gagacgtcgt cgagcatcaa tgcactcaag gcagccgagt ccttggtgat 5641 ccaccttctc ggcgaacatc agcagcatct ggcccagcgc tttgcgcgta gtgccgatca 5701 gcgttttgca gacgagtcac tgtgggcagt gctcgacacc ggggaaccgg tgctgcacgg 5761 cacccccagc tggatgcgcg tcaaggtcga ccagctgatc cctgtcggcg accacacgct 5821 ggtcatcgga ctcgtcacgc gggttcacgc cgaagaagac gacgaatccg ctgccgcgcc 5881 gctgctctac cacgagggca agtactaccg cccgactccg ttaggtcaat agccaggcat 5941 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 6001 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 6061 atactagagg acaccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgcccg 6121 gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag 6181 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct 6241 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc 6301 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg 6361 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt 6421 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg 6481 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag 6541 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct 6601 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg 6661 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct 6721 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag 6781 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa 6841 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg 6901 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg 6961 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag 7021 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag 7081 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg 7141 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 7201 caggtttccc gactggaaag cgggcagtga tactagagcc aggcatcaaa taaaacgaaa 7261 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 7321 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //