BBa_K1974033
1
BBa_K1974033
T7 Promoter+RBS+Hv1a+GS linker+snowdrop-lectin+linker+6X His-Tag
2016-10-12T11:00:00Z
2017-03-28T10:01:59Z
Artificial Synthesis
well
false
false
_2441_
30645
30645
9
true
well
false
YU-CHUN WU
component2532936
1
BBa_B0034
component2532939
1
BBa_K1223006
component2532934
1
BBa_I712074
annotation2532939
1
BBa_K1223006
range2532939
1
75
101
annotation2532934
1
BBa_I712074
range2532934
1
1
46
annotation2532936
1
BBa_B0034
range2532936
1
55
66
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_I712074
1
BBa_I712074
T7 promoter (strong promoter from T7 bacteriophage)
2007-10-21T11:00:00Z
2015-08-31T04:07:46Z
T7 bacteriophage
T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
false
false
_130_
0
1856
9
In stock
false
true
Rok Gaber
BBa_K1223006
1
BBa_K1223006
His-Tag
2013-09-06T11:00:00Z
2015-05-08T01:09:43Z
synthetic
c-term His tag for purification and identification of protein of interest.
false
false
_1537_
0
18143
9
It's complicated
false
purification and identification of protein of interest.
false
Orr Schlesinger
annotation2335270
1
6X His-Tag
range2335270
1
1
24
annotation2335271
1
stop codon
range2335271
1
25
27
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1223006_sequence
1
gtgcaccaccaccaccatcacgtgtaa
BBa_K1974033_sequence
1
taatacgactcactatagggaatacaagctacttgttctttttgcatactagagaaagaggagaaatactagaggtgcaccaccaccaccatcacgtgtaa
BBa_I712074_sequence
1
taatacgactcactatagggaatacaagctacttgttctttttgca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z