LOCUS       BBa_K1976033             319 bp    DNA     linear       17-May-2024
DEFINITION  Inducible generator for colicin E2 immunity protein (+amber codon)
ACCESSION   BBa_K1976033
VERSION     BBa_K1976033.1  
FEATURES             Location/Qualifiers
     misc_feature    21..32
                     /label=B0034
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     promoter        1..32
                     /label=BBa_K525998
     misc_feature    45..48
                     /label=conserved
     RBS             41..52
                     /label=BBa_B0034
     misc_feature    86..88
                     /label=amber codon
     CDS             59..319
                     /label=ImE2
     CDS             59..319
                     /label=BBa_K1976026
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagag aaagaggaga aatactagat
       61 ggaactgaaa catagtatta gtgattagac cgaggctgaa tttctggagt ttgtaaaaaa
      121 aatatgtaga gctgaaggtg ctactgaaga ggatgacaat aaattagtga gagagtttga
      181 gcgattaact gagcacccag atggttcaga tctgatttat tatcctcgcg atgacaggga
      241 agatagtcct gaagggattg tcaaggaaat taaagaatgg cgagctgcta acggtaagtc
      301 aggatttaaa cagggctga
//