LOCUS       BBa_K1981202            2209 bp    DNA     linear       01-Jun-2024
DEFINITION  Autoinducer-2 Response Device B
ACCESSION   BBa_K1981202
VERSION     BBa_K1981202.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     CDS             136..1089
                     /label=BBa_K091001
     promoter        1098..1346
                     /label=lsr promoter 
     promoter        1098..1346
                     /label=BBa_K1981101
     CDS             1353..2072
                     /label=GFP protein
     CDS             1353..2072
                     /label=BBa_E0040
     stem_loop       2092..2135
                     /label=stem_loop
     misc_feature    2081..2160
                     /label=BBa_B0010
     terminator      2081..2160
                     /label=BBa_B0010
     stem_loop       2176..2195
                     /label=T7 TE
     misc_feature    2202..2202
                     /label=stop
     polyA_site      2196..2209
                     /label=polya
     misc_feature    2169..2209
                     /label=BBa_B0012
     terminator      2169..2209
                     /label=BBa_B0012
     terminator      2081..2209
                     /label=BBa_B0015
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagatgac aatcaacgat tcggcaattt cagaacaggg aatgtgtgaa
      181 gaagaacagg tcgcgcggat cgcgtggttt tactatcacg acgggctgac ccagagcgag
      241 atcagcgatc gtctcggcct gacacgtttg aaagtgtcgc gattgctgga gaaagggcat
      301 cagtccggca ttattcgcgt acagattaat tctcgctttg aaggctgtct ggaatatgaa
      361 actcaattac gtcgtcagtt ttcgctgcaa catgtccggg tgatccctgg gcttgcggat
      421 gctgatgtcg gtgggcgact ggggataggc gcggcgcata tgttgatgag tttacttcaa
      481 ccacaacaga tgctggcgat tggttttggc gaggcaacca tgaatacgct gcaacgctta
      541 agtggtttta tttcgtcaca gcaaattcgc ctggtcacgc tctccggtgg cgtcggttct
      601 tatatgacgg gaatcgggca gcttaacgcg gcgtgcagtg tgaatattat tccggctccg
      661 ttgcgggcat cctccgctga cattgcccgt acgctaaaaa atgaaaattg cgtcaaagat
      721 gttctgttag ccgcgcaagc agcggatgtg gcgattgtcg gcattggtgc tgtgagtcaa
      781 caggacgatg cgacaatcat tcgctccggt tatatcagcc agggcgaaca gttaatgatt
      841 ggccgaaaag gggcggttgg cgacatttta ggctactttt ttgatgcaaa aggtgacgtt
      901 gtcacgaata tcaaaataca taacgaactg attggcttac ctttaagcgc gctgaagacc
      961 atacccgtcc gggttggcgt ggcaggggga gaaaataaag ccgaagcaat tgccgctgca
     1021 atgaaaggcg gttatatcaa cgcactggtt accgatcagg acacagcagc ggcgatttta
     1081 cgtagttaat actagagcaa ttcattcttc actttgaaca tatttaaatc tttaatgcaa
     1141 ttgttcagtt cttgcgcatt tatatctgtg atggcagcca cagtttgact ctacgagcat
     1201 gaacaaacgc aaccgtgaaa atcaaaatag cataaattgt gatctattcg tcggaaatat
     1261 gtgcaatgtc cacctaaggt tatgaacaaa ttaaaagcag aaatacattt gttcaaaact
     1321 cacctgcaaa actgaacggg ggaaattact agatgcgtaa aggagaagaa cttttcactg
     1381 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
     1441 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
     1501 ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat
     1561 gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg
     1621 aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg
     1681 ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt
     1741 ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac tcacacaatg
     1801 tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca
     1861 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg
     1921 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag
     1981 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta
     2041 cacatggcat ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga
     2101 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2161 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//