LOCUS       BBa_K1988013            2248 bp    DNA     linear       19-May-2024
DEFINITION  his-tagged PPK1 homologue w/ Anderson Promoter J23106
ACCESSION   BBa_K1988013
VERSION     BBa_K1988013.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     RBS             44..56
                     /label=BBa_J34803
     CDS             63..2204
                     /label=BBa_K1988012
     misc_feature    2213..2242
                     /label=10x-Histidine Tag
     misc_feature    2243..2245
                     /label=Stop
     misc_feature    2246..2248
                     /label=Stop
     misc_feature    2213..2248
                     /label=BBa_K844000
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagtcacaca ggaaagtact
       61 agatgaatgc tccggatctg gctggcgatc ttttggaacc aacgggcacg cagactgtca
      121 acgacggaga aagcgacggg cttcccgatg gacgtttcct ggatcgtgaa ttgtcttggt
      181 tagcgttcaa ctctcgtgtg cttgatctgg cgaaagacgc acagcgcgtc ccgcttttag
      241 aacgcgctaa gtttttagcg atttttgcaa ataatttgga cgaatttttc atggtccgcg
      301 tagcggggct taaacgtcgt atcgctgccg gtgtggctgt tcgcaccgtt gctggcttaa
      361 tgccccgtga ggtgcatgag accattctga cccgcacgcg cgaattagtc accgagcaca
      421 gtcgcgtgtt cgaagaggag attcgccctg agctggcggc ccacgggatc gaaatcttgc
      481 actggcacga gttaacacca gacgagatgg aacgcatgcg cgtcctgttc gcagagcgca
      541 tctttccagt gttaacccct ctggccgtcg acccttcaca tcccttcccc tatatcagcg
      601 ggctgtcgat taatttagcg gtattggtaa aaaatccatc gaccggagta cgccagtttg
      661 cacgtgttaa agttccgtcc gtattacccc gctttgtgcg cttagccgaa ggtcgctttg
      721 tggcattaga ggatgtgatc gcgcgccatc ttgatcaatt attcacgggg atgcaggtcg
      781 ttcagcatca cgtcttccgt gttactcgta acgaggatgt tgaagtagaa gaggatgatg
      841 cagagaatct gcttgtagca ttggagcgcg agttactgcg ccgtaaggtg ggacgtcctc
      901 ctgttcgctt ggaggttgaa gatgatatcg actcgaagat gctggagttg ttaattagtg
      961 aacttgatat ttccgaaaag gaggtatttg cacttcccgg gcccctggat ctgcgcggtc
     1021 ttttttcact ggccgatctt gatcgcgctg aacttaaata tccggcattc ctgccaagta
     1081 ctcatcctca tctggcggaa gtcgaaacag ccaaaccggc agaccttttt gccgcgttaa
     1141 aacagcgtga tgtactttta catcaccctt atgattcatt cgctacatcc gtgcaacgct
     1201 ttattgaaca ggccgctgac gacccacagg tgttagctat taaacagacg ttatatcgca
     1261 cttccggcga ctcaccgatt attgacgctc ttattgaggc tgccgaagcg ggtaagcaag
     1321 tattggcttt agttgaaatt aaagcccgct ttgacgagca agccaacatc gcctgggccc
     1381 gtcgtctgga gcagcacggg tgtcacgttg tttatggatt gttaggcctg aagactcatt
     1441 gtaagctgtc aatggttgtt cgtgacgaac cggacggatt acgtcgctac gcccacatcg
     1501 gcactggtaa ttataacccc aaaaccgccc gcctgtacga agatatgggt ttgcttaccg
     1561 ccaatcctat tattactgac gatatcggcc gtttgttcaa ccatatgagt ggcatgtcac
     1621 aggaaacaca gtatcgtcgt ctgcttgtag ctccccatgg gattcgcacc ggcttgattg
     1681 accgtatcaa taatgaaatt gctaaccatg aagatggcaa gcccgcgggc attaagatta
     1741 aggttaacag tctggtcgat gaaactgtaa ccgatgccct ttaccgtgca tcccaagcgg
     1801 gagtacccgt cgacttgtgg gtacgtggta tttgcaccat tcgtcctggt gtacccgggc
     1861 tgagcgaaaa tattcgtgta cgcagcattt taggccgctt cttggagcac tctcgcttgt
     1921 tttggtttgc caacggcggt caagcctcgg ttggaattgg gtccgcagac ttaatgcacc
     1981 gtaatcttga ccgtcgcgtc gaaacacttg cttcaatcac caatccagct cacgtggcgg
     2041 agattgaggg gcttttcgat ttggctttcg accctggtac ggcggcctgg ttgttgaacg
     2101 ccgacgggac ttggacacaa cgtacaacgg acgaagatgg cgagccattg ctggacatgc
     2161 aggctcatct gatcagtgtg aaaagccgtc gtcgtgcagc taagtactag agcatcatca
     2221 ccatcaccac catcatcacc attaataa
//