LOCUS       BBa_K199029             1350 bp    DNA     linear       01-Jun-2024
DEFINITION  pBad-RBS-Tet
ACCESSION   BBa_K199029
VERSION     BBa_K199029.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     RBS             139..153
                     /label=RBS-1\Strong
     RBS             146..150
                     /label=RBS
     misc_feature    139..153
                     /label=BBa_B0030
     RBS             139..153
                     /label=BBa_B0030
     CDS             160..1350
                     /label=tetA(C) forward
     primer_bind     160..183
                     /label=Fwd primer J31007
     primer_bind     1331..1350
                     /label=Rev primer J31007
     misc_feature    1348..1350
                     /label=stop
     CDS             160..1350
                     /label=BBa_J31007
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagat taaagaggag aaatactaga tgaaatctaa caatgcgctc
      181 atcgtcatcc tcggcaccgt caccctggat gctgtaggca taggcttggt tatgccggta
      241 ctgccgggcc tcttgcggga tatcgtccat tccgacagca tcgccagtca ctatggcgtg
      301 ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc
      361 gaccgctttg gccgccgccc agtcctgctc gcttcgctac ttggagccac tatcgactac
      421 gcgatcatgg cgaccacacc cgtcctgtgg atcctctacg ccggacgcat cgtggccggc
      481 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
      541 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
      601 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
      661 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
      721 gagcgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
      781 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
      841 gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg
      901 atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact
      961 ggccccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac
     1021 gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg
     1081 attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag
     1141 gtagatgacg accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact
     1201 tcgatcattg gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac
     1261 gggttggcat ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc
     1321 ggtgcatgga gccgggccac ctcgacctaa
//