LOCUS       BBa_K199127              824 bp    DNA     linear       01-Jun-2024
DEFINITION  TT-pLux-RBS-LuxI optimized
ACCESSION   BBa_K199127
VERSION     BBa_K199127.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     misc_feature    138..157
                     /label=LuxR/HSL
     promoter        157..162
                     /label=-35
     promoter        179..184
                     /label=-10
     misc_feature    190..190
                     /label=start
     misc_feature    138..192
                     /label=BBa_R0062
     promoter        138..192
                     /label=BBa_R0062
     misc_feature    205..208
                     /label=conserved
     RBS             201..212
                     /label=BBa_B0034
     CDS             219..824
                     /label=BBa_K199125
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta
      181 tagtcgaata aatactagag aaagaggaga aatactagat ggcaattgca agcggtcgtg
      241 caaccattat gattaaaaaa agcgattttc tggccattcc gagcgaagaa tataaaggta
      301 ttctgagcct gcgctatcag gtttttaaac agcgtctgga atgggatctg gtggttgaaa
      361 ataatctgga aagtgatgaa tatgataata gcaatgccga atatatttat gcctgtgatg
      421 ataccgaaaa tgttagcggt tgttggcgtc tgctgccgac caccggtgat tatatgctga
      481 aaagcgtttt tccggaactg ctgggtcagc agagcgcacc gaaagatccg aatattgttg
      541 aactgagccg ttttgccgtg ggtaaaaata gcagcaaaat taataatagc gccagcgaaa
      601 ttaccatgaa actgtttgaa gccatttata aacatgccgt tagccagggt attaccgaat
      661 atgttaccgt taccagcacc gcaattgaac gttttctgaa acgtattaaa gtgccgtgtc
      721 atcgtattgg cgataaagaa attcatgttc tgggcgatac caaaagcgtt gttctgagca
      781 tgccgattaa tgaacagttt aaaaaagccg tgctgaacta ataa
//