LOCUS       BBa_K199166              948 bp    DNA     linear       30-Apr-2024
DEFINITION  pT7+RBS+EYFP+TT
ACCESSION   BBa_K199166
VERSION     BBa_K199166.1  
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label=Transcription Start Site
     misc_feature    1..11
                     /label=Polymerase Binding Region
     misc_feature    12..23
                     /label=Initiation Region
     promoter        1..23
                     /label=BBa_R0085
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     misc_feature    806..811
                     /label=stop
     misc_feature    767..805
                     /label=AAV
     misc_feature    50..811
                     /label=BBa_E0034
     stem_loop       831..874
                     /label=stem_loop
     misc_feature    820..899
                     /label=BBa_B0010
     terminator      820..899
                     /label=BBa_B0010
     stem_loop       915..934
                     /label=T7 TE
     misc_feature    941..941
                     /label=stop
     polyA_site      935..948
                     /label=polya
     misc_feature    908..948
                     /label=BBa_B0012
     terminator      908..948
                     /label=BBa_B0012
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggtgagcaa
       61 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      121 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      181 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      241 cttcggctac ggcctgcaat gcttcgcccg ctaccccgac cacatgaagc tgcacgactt
      301 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      361 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      421 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      481 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
      541 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      601 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta
      661 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
      721 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagaggc ctgctgcaaa
      781 cgacgaaaac tacgctgctg ctgtttaata atactagagc caggcatcaa ataaaacgaa
      841 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
      901 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//