LOCUS BBa_K199194 3478 bp DNA linear 01-Jun-2024 DEFINITION TT-pLux-RBS-LuxI 2 sites-RBS-GFP-RBS-RFP-pLacI-RBS-LuxR ACCESSION BBa_K199194 VERSION BBa_K199194.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 misc_feature 138..157 /label=LuxR/HSL promoter 157..162 /label=-35 promoter 179..184 /label=-10 misc_feature 190..190 /label=start misc_feature 138..192 /label=BBa_R0062 promoter 138..192 /label=BBa_R0062 misc_feature 205..208 /label=conserved RBS 201..212 /label=BBa_B0034 misc_feature 222..229 /label=AsiSI Site misc_feature 235..242 /label=AscI Site CDS 219..845 /label=BBa_K199130 misc_feature 1..845 /label=BBa_S04424 RBS 854..868 /label=RBS-1\Strong RBS 861..865 /label=RBS misc_feature 854..868 /label=BBa_B0030 RBS 854..868 /label=BBa_B0030 CDS 875..1594 /label=GFP protein CDS 875..1594 /label=BBa_E0040 misc_feature 854..1594 /label=BBa_E5500 misc_feature 1607..1610 /label=conserved RBS 1603..1614 /label=BBa_B0034 CDS 1621..2295 /label=mrfp1 misc_feature 2302..2326 /label=Help:Barcodes CDS 1621..2326 /label=BBa_E1010 misc_feature 1603..2326 /label=BBa_S04419 CDS 2335..2422 /label=end of LacI coding region (inactive) misc_feature 2335..2534 /label=BBa_R0010 misc_feature 2423..2460 /label=CAP binding site promoter 2471..2476 /label=-35 promoter 2495..2500 /label=-10 misc_feature 2500..2534 /label=LacI binding site misc_feature 2507..2507 /label=start promoter 2335..2534 /label=BBa_R0010 misc_feature 2547..2550 /label=conserved RBS 2543..2554 /label=BBa_B0034 misc_feature 2561..2562 /label=prefix misc_feature 2734..2734 /label=T misc_feature 3052..3052 /label=A CDS 2561..3310 /label=luxR misc_feature 2561..3316 /label=BBa_C0062 misc_feature 3317..3341 /label=Help:Barcodes CDS 2561..3316 /label=BBa_C0062 stem_loop 3361..3404 /label=stem_loop misc_feature 3350..3429 /label=BBa_B0010 terminator 3350..3429 /label=BBa_B0010 stem_loop 3445..3464 /label=T7 TE misc_feature 3471..3471 /label=stop polyA_site 3465..3478 /label=polya misc_feature 3438..3478 /label=BBa_B0012 terminator 3438..3478 /label=BBa_B0012 misc_feature 2543..3478 /label=BBa_I0462 misc_feature 2335..3478 /label=BBa_K109702 misc_feature 1603..3478 /label=BBa_K199173 misc_feature 854..3478 /label=BBa_S04473 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta 181 tagtcgaata aatactagag aaagaggaga aatactagat ggcgatcgca tctgggcgcg 241 ccgcaattgc aagcggtcgt gcaaccatta tgattaaaaa aagcgatttt ctggccattc 301 cgagcgaaga atataaaggt attctgagcc tgcgctatca ggtttttaaa cagcgtctgg 361 aatgggatct ggtggttgaa aataatctgg aaagtgatga atatgataat agcaatgccg 421 aatatattta tgcctgtgat gataccgaaa atgttagcgg ttgttggcgt ctgctgccga 481 ccaccggtga ttatatgctg aaaagcgttt ttccggaact gctgggtcag cagagcgcac 541 cgaaagatcc gaatattgtt gaactgagcc gttttgccgt gggtaaaaat agcagcaaaa 601 ttaataatag cgccagcgaa attaccatga aactgtttga agccatttat aaacatgccg 661 ttagccaggg tattaccgaa tatgttaccg ttaccagcac cgcaattgaa cgttttctga 721 aacgtattaa agtgccgtgt catcgtattg gcgataaaga aattcatgtt ctgggcgata 781 ccaaaagcgt tgttctgagc atgccgatta atgaacagtt taaaaaagcc gtgctgaact 841 aataatacta gagattaaag aggagaaata ctagatgcgt aaaggagaag aacttttcac 901 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt 961 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac 1021 tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca 1081 atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc 1141 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg 1201 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga 1261 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa 1321 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca 1381 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg 1441 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa 1501 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat 1561 tacacatggc atggatgaac tatacaaata ataatactag agaaagagga gaaatactag 1621 atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt 1681 tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt 1741 acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc 1801 ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg 1861 gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa 1921 gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac 1981 aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc 2041 atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa 2101 atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc 2161 tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac 2221 atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt 2281 cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact agagcaatac 2341 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc 2401 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg 2461 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat 2521 aacaatttca cacatactag agaaagagga gaaatactag atgaaaaaca taaatgccga 2581 cgacacatac agaataatta ataaaattaa agcttgtaga agcaataatg atattaatca 2641 atgcttatct gatatgacta aaatggtaca ttgtgaatat tatttactcg cgatcattta 2701 tcctcattct atggttaaat ctgatatttc aatcctagat aattacccta aaaaatggag 2761 gcaatattat gatgacgcta atttaataaa atatgatcct atagtagatt attctaactc 2821 caatcattca ccaattaatt ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc 2881 aaatgtaatt aaagaagcga aaacatcagg tcttatcact gggtttagtt tccctattca 2941 tacggctaac aatggcttcg gaatgcttag ttttgcacat tcagaaaaag acaactatat 3001 agatagttta tttttacatg cgtgtatgaa cataccatta attgttcctt ctctagttga 3061 taattatcga aaaataaata tagcaaataa taaatcaaac aacgatttaa ccaaaagaga 3121 aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct tgggatattt caaaaatatt 3181 aggttgcagt gagcgtactg tcactttcca tttaaccaat gcgcaaatga aactcaatac 3241 aacaaaccgc tgccaaagta tttctaaagc aattttaaca ggagcaattg attgcccata 3301 ctttaaaaat taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa 3361 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 3421 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //