LOCUS       BBa_K1992004            1860 bp    DNA     linear       01-Jun-2024
DEFINITION  Tar expression system (promoter+RBS+coding+terminator)
ACCESSION   BBa_K1992004
VERSION     BBa_K1992004.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..64
                     /label=ATG
     CDS             62..1723
                     /label=Tar coding region
     misc_feature    1721..1723
                     /label=TGA
     CDS             62..1723
                     /label=BBa_K777000
     stem_loop       1743..1786
                     /label=stem_loop
     misc_feature    1732..1811
                     /label=BBa_B0010
     terminator      1732..1811
                     /label=BBa_B0010
     stem_loop       1827..1846
                     /label=T7 TE
     misc_feature    1853..1853
                     /label=stop
     polyA_site      1847..1860
                     /label=polya
     misc_feature    1820..1860
                     /label=BBa_B0012
     terminator      1820..1860
                     /label=BBa_B0012
     terminator      1732..1860
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgattaac cgtatccgcg tagtcacgct gttggtaatg gtgctggggg tattcgcact
      121 gttacagctt atttccggca gtctgttttt ttcttccctt caccatagcc agaagagctt
      181 tgtggtttcc aatcaattac gggaacagca gggcgagctg acgtcaacct gggatttaat
      241 gctgcaaacg cgcattaacc tgagtcgttc agcggtacgg atgatgatgg attcctccaa
      301 tcaacaaagt aacgccaaag ttgaattgct cgatagcgcc aggaaaacat tggcgcaggc
      361 agcgacgcat tataaaaaat tcaaaagcat ggcaccgtta cctgaaatgg tcgctaccag
      421 tcgtaatatt gatgaaaaat ataaaaacta ttacacagcg ttaactgaac tgattgatta
      481 cctagattat ggcaatactg gagcttattt cgctcagcca acccagggaa tgcaaaatgc
      541 aatgggcgaa gcgtttgctc agtacgccct cagcagtgaa aaactgtatc gcgatatcgt
      601 cactgacaac gcagatgatt accgatttgc ccagtggcaa ctggcggtta tcgcgctggt
      661 ggtggtattg attctgctgg tggcgtggta cggcattcgc cgtatgttgc ttactccgct
      721 ggcaaaaatt attgctcaca ttcgcgaaat cgccggtggt aacctggcga ataccctgac
      781 cattgacggg cgcagtgaaa tgggcgacct ggcgcagagc gtttcacata tgcaacgctc
      841 tttgactgac accgtcactc atgtccgcga aggttcagat gccatctatg ccggtacccg
      901 tgaaattgcg gcgggcaaca ccgatctttc ctcccgtact gaacagcagg catccgcgct
      961 ggaagaaact gccgccagca tggagcagct caccgcgaca gtgaagcaaa acgccgataa
     1021 cgcccgccag gcctcgcaac tggcgcaaag tgcctccgac accgcccagc acggcggcaa
     1081 agtggtggat ggcgtagtga aaacgatgca tgagatcgcc gatagttcga agaaaattgc
     1141 cgacattatc agcgttatcg acggtattgc cttccagact aatatcctcg cgctgaatgc
     1201 cgcggttgaa gccgcgcgtg cgggtgaaca gggccgtggt tttgccgtgg tggcgggtga
     1261 agtgcgtaat cttgccagtc gcagcgccca ggcggcaaaa gagatcaaag ccctcattga
     1321 agactccgtc tcacgcgttg ataccggttc ggtgctggtc gaaagcgccg gggaaacaat
     1381 gaacaatatc gtcaatgctg tcactcgcgt gactgacatt atgggcgaga ttgcatcggc
     1441 atcggatgaa cagagccgtg gcatcgatca agtcgcattg gcggtttcgg aaatggatcg
     1501 cgtcacgcaa cagaacgcat cgctggtgca ggaatcagct gccgccgccg ctgcgctgga
     1561 agaacaggcg agtcgtttaa cgcaagcagt ttccgcgttc cgtctggcag ccagcccact
     1621 caccaataaa ccgcaaacac catcccgtcc tgccagtgag caaccaccgg ctcagccacg
     1681 actgcgaatt gctgaacaag atccaaactg ggaaacattt tgatactaga gccaggcatc
     1741 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1801 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//