LOCUS       BBa_K1993009            3366 bp    DNA     linear       01-Jun-2024
DEFINITION  CXCR4-T2A-Luciferase-IRES-eGFP
ACCESSION   BBa_K1993009
VERSION     BBa_K1993009.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=start codon for CXCR4
     misc_feature    1069..1122
                     /label=T2A
     CDS             4..1068
                     /label=CXCR4
     misc_feature    1123..1125
                     /label=start codon for Luciferase
     CDS             1126..2055
                     /label=Luciferase
     misc_feature    2056..2058
                     /label=stop codon for Luciferase
     CDS             1..2058
                     /label=BBa_K1993032
     RBS             2059..2646
                     /label=IRES
     RBS             2059..2646
                     /label=BBa_K1993016
     misc_feature    2647..2649
                     /label=start codon
     CDS             2650..3363
                     /label=eGFP
     misc_feature    3364..3366
                     /label=stop codon
     misc_feature    2647..3366
                     /label=BBa_K1993017
ORIGIN
        1 atgtccattc ctttgcctct tttgcagata tacacttcag ataactacac cgaggaaatg
       61 ggctcagggg actatgactc catgaaggaa ccctgtttcc gtgaagaaaa tgctaatttc
      121 aataaaatct tcctgcccac catctactcc atcatcttct taactggcat tgtgggcaat
      181 ggattggtca tcctggtcat gggttaccag aagaaactga gaagcatgac ggacaagtac
      241 aggctgcacc tgtcagtggc cgacctcctc tttgtcatca cgcttccctt ctgggcagtt
      301 gatgccgtgg caaactggta ctttgggaac ttcctatgca aggcagtcca tgtcatctac
      361 acagtcaacc tctacagcag tatcctcatc ctggccttca tcagtctgga ccgctacctg
      421 gccatcgtcc acgccaccaa cagtcagagg ccaaggaagc tgttggctga agaggtggtc
      481 tatgttggcg tctggatccc tgccctcctg ccgactattc ccgacttcat ctttgccaac
      541 gtcagtgagg cagatgacag atatatctgt gaccgcttct accccaatga cttgtgggtg
      601 gttgtgttcc agtttcagca catcatggtt ggccttatcc tgcctggtat tgtcatcctg
      661 tcctgctatt gcattatcat ctccaagctg tcacactcca agggccacca gaagcgcaag
      721 gccctcaaga ccacagtcat cctcatcctg gctttcttcg cctgttggct gccttactac
      781 attgggatca gcatcgactc cttcatcctc ctggaaatca tcaagcaagg gtgtgagttt
      841 gagaacactg tgcacaagtg gatttccatc accgaggccc tagctttctt ccactgttgt
      901 ctgaacccca tcctctatgc tttccttgga gccaaattta aaacctctgc ccagcacgca
      961 ctcacctctg tgagcagagg gtccagcctc aagatcctct ccaaaggaaa gcgaggtgga
     1021 cattcatctg tttccactga gtctgagtct tcaagttttc actccagcga gggcagggga
     1081 agtcttctaa catgcgggga cgtggaggaa aatcccggcc ccatgacttc gaaagtttat
     1141 gatccagaac aaaggaaacg gatgataact ggtccgcagt ggtgggccag atgtaaacaa
     1201 atgaatgttc ttgattcatt tattaattat tatgattcag aaaaacatgc agaaaatgct
     1261 gttatttttt tacatggtaa cgcggcctct tcttatttat ggcgacatgt tgtgccacat
     1321 attgagccag tagcgcggtg tattatacca gatcttattg gtatgggcaa atcaggcaaa
     1381 tctggtaatg gttcttatag gttacttgat cattacaaat atcttactgc atggtttgaa
     1441 cttcttaatt taccaaagaa gatcattttt gtcggccatg attggggtgc ttgtttggca
     1501 tttcattata gctatgagca tcaagataag atcaaagcaa tagttcacgc tgaaagtgta
     1561 gtagatgtga ttgaatcatg ggatgaatgg cctgatattg aagaagatat tgcgttgatc
     1621 aaatctgaag aaggagaaaa aatggttttg gagaataact tcttcgtgga aaccatgttg
     1681 ccatcaaaaa tcatgagaaa gttagaacca gaagaatttg cagcatatct tgaaccattc
     1741 aaagagaaag gtgaagttcg tcgtccaaca ttatcatggc ctcgtgaaat cccgttagta
     1801 aaaggtggta aacctgacgt tgtacaaatt gttaggaatt ataatgctta tctacgtgca
     1861 agtgatgatt taccaaaaat gtttattgaa tcggatccag gattcttttc caatgctatt
     1921 gttgaaggcg ccaagaagtt tcctaatact gaatttgtca aagtaaaagg tcttcatttt
     1981 tcgcaagaag atgcacctga tgaaatggga aaatatatca aatcgttcgt tgagcgagtt
     2041 ctcaaaaatg aacaataagc ccctctccct cccccccccc taacgttact ggccgaagcc
     2101 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     2161 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     2221 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     2281 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     2341 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     2401 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     2461 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     2521 ctgatctggg gcctcggtac acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     2581 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
     2641 acaaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     2701 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     2761 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     2821 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     2881 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     2941 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     3001 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     3061 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     3121 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     3181 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     3241 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     3301 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     3361 aagtaa
//