LOCUS       BBa_K1993029            3395 bp    DNA     linear       14-Jun-2024
DEFINITION  CXCR4-T2A-Luciferase-IRES-eGFP
ACCESSION   BBa_K1993029
VERSION     BBa_K1993029.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=start codon for CXCR4
     CDS             4..1068
                     /label=CXCR4
     misc_feature    1069..1122
                     /label=T2A
     misc_feature    1123..1125
                     /label=start codon for Luciferase
     CDS             1126..2055
                     /label=Luciferase
     misc_feature    2056..2058
                     /label=stop codon for Luciferase
     misc_feature    2059..2087
                     /label=attB2
     RBS             2088..2675
                     /label=IRES
     misc_feature    2676..2678
                     /label=start codon for eGFP
     CDS             2679..3392
                     /label=eGFP
     misc_feature    3393..3395
                     /label=stop codon for eGFP
ORIGIN
        1 atgtccattc ctttgcctct tttgcagata tacacttcag ataactacac cgaggaaatg
       61 ggctcagggg actatgactc catgaaggaa ccctgtttcc gtgaagaaaa tgctaatttc
      121 aataaaatct tcctgcccac catctactcc atcatcttct taactggcat tgtgggcaat
      181 ggattggtca tcctggtcat gggttaccag aagaaactga gaagcatgac ggacaagtac
      241 aggctgcacc tgtcagtggc cgacctcctc tttgtcatca cgcttccctt ctgggcagtt
      301 gatgccgtgg caaactggta ctttgggaac ttcctatgca aggcagtcca tgtcatctac
      361 acagtcaacc tctacagcag tatcctcatc ctggccttca tcagtctgga ccgctacctg
      421 gccatcgtcc acgccaccaa cagtcagagg ccaaggaagc tgttggctga agaggtggtc
      481 tatgttggcg tctggatccc tgccctcctg ccgactattc ccgacttcat ctttgccaac
      541 gtcagtgagg cagatgacag atatatctgt gaccgcttct accccaatga cttgtgggtg
      601 gttgtgttcc agtttcagca catcatggtt ggccttatcc tgcctggtat tgtcatcctg
      661 tcctgctatt gcattatcat ctccaagctg tcacactcca agggccacca gaagcgcaag
      721 gccctcaaga ccacagtcat cctcatcctg gctttcttcg cctgttggct gccttactac
      781 attgggatca gcatcgactc cttcatcctc ctggaaatca tcaagcaagg gtgtgagttt
      841 gagaacactg tgcacaagtg gatttccatc accgaggccc tagctttctt ccactgttgt
      901 ctgaacccca tcctctatgc tttccttgga gccaaattta aaacctctgc ccagcacgca
      961 ctcacctctg tgagcagagg gtccagcctc aagatcctct ccaaaggaaa gcgaggtgga
     1021 cattcatctg tttccactga gtctgagtct tcaagttttc actccagcga gggcagggga
     1081 agtcttctaa catgcgggga cgtggaggaa aatcccggcc ccatgacttc gaaagtttat
     1141 gatccagaac aaaggaaacg gatgataact ggtccgcagt ggtgggccag atgtaaacaa
     1201 atgaatgttc ttgattcatt tattaattat tatgattcag aaaaacatgc agaaaatgct
     1261 gttatttttt tacatggtaa cgcggcctct tcttatttat ggcgacatgt tgtgccacat
     1321 attgagccag tagcgcggtg tattatacca gatcttattg gtatgggcaa atcaggcaaa
     1381 tctggtaatg gttcttatag gttacttgat cattacaaat atcttactgc atggtttgaa
     1441 cttcttaatt taccaaagaa gatcattttt gtcggccatg attggggtgc ttgtttggca
     1501 tttcattata gctatgagca tcaagataag atcaaagcaa tagttcacgc tgaaagtgta
     1561 gtagatgtga ttgaatcatg ggatgaatgg cctgatattg aagaagatat tgcgttgatc
     1621 aaatctgaag aaggagaaaa aatggttttg gagaataact tcttcgtgga aaccatgttg
     1681 ccatcaaaaa tcatgagaaa gttagaacca gaagaatttg cagcatatct tgaaccattc
     1741 aaagagaaag gtgaagttcg tcgtccaaca ttatcatggc ctcgtgaaat cccgttagta
     1801 aaaggtggta aacctgacgt tgtacaaatt gttaggaatt ataatgctta tctacgtgca
     1861 agtgatgatt taccaaaaat gtttattgaa tcggatccag gattcttttc caatgctatt
     1921 gttgaaggcg ccaagaagtt tcctaatact gaatttgtca aagtaaaagg tcttcatttt
     1981 tcgcaagaag atgcacctga tgaaatggga aaatatatca aatcgttcgt tgagcgagtt
     2041 ctcaaaaatg aacaataagg ggaccacttt gtacaagaaa gctgggtgcc cctctccctc
     2101 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     2161 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     2221 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     2281 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     2341 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     2401 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     2461 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     2521 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtaca catgctttac
     2581 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     2641 cctttgaaaa acacgatgat aatatggcca caaccatggt gagcaagggc gaggagctgt
     2701 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     2761 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     2821 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     2881 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     2941 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     3001 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     3061 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     3121 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     3181 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     3241 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     3301 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     3361 ggatcactct cggcatggac gagctgtaca agtga
//