LOCUS       BBa_K1993030            3443 bp    DNA     linear       01-Jun-2024
DEFINITION  CXCR5-T2A-Luciferase-IRES-eGFP
ACCESSION   BBa_K1993030
VERSION     BBa_K1993030.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=start codon for CXCR5
     CDS             4..1116
                     /label=CXCR5
     misc_feature    1117..1170
                     /label=T2A
     misc_feature    1171..1173
                     /label=start codon for Luciferase
     CDS             1174..2103
                     /label=Luciferase
     misc_feature    2104..2106
                     /label=stop codon for Luciferase
     RBS             2136..2723
                     /label=IRES
     misc_feature    2107..2135
                     /label=attB2
     misc_feature    2724..2726
                     /label=start codon for eGFP
     CDS             2727..3440
                     /label=eGFP
     misc_feature    3441..3443
                     /label=stop codon for eGFP
ORIGIN
        1 atgaactacc cgctaacgct ggaaatggac ctcgagaacc tggaggacct gttctgggaa
       61 ctggacagat tggacaacta taacgacacc tccctggtgg aaaatcatct ctgccctgcc
      121 acagaggggc ccctcatggc ctccttcaag gccgtgttcg tgcccgtggc ctacagcctc
      181 atcttcctcc tgggcgtgat cggcaacgtc ctggtgctgg tgatcctgga gcggcaccgg
      241 cagacacgca gttccacgga gaccttcctg ttccacctgg ccgtggccga cctcctgctg
      301 gtcttcatct tgccctttgc cgtggccgag ggctctgtgg gctgggtcct ggggaccttc
      361 ctctgcaaaa ctgtgattgc cctgcacaaa gtcaacttct actgtagcag cctgctcctg
      421 gcctgcatcg ccgtggaccg ctacctggcc attgtccacg ccgtccatgc ctaccgccac
      481 cgccgcctcc tctccatcca catcacctgt gggaccatct ggctggtggg cttcctcctt
      541 gccttgccag agattctctt cgccaaagtc agccaaggcc atcacaacaa ctccctgcca
      601 cgttgcacct tctcccaaga gaaccaagca gaaacgcatg cctggttcac ctcccgattc
      661 ctctaccatg tggcgggatt cctgctgccc atgctggtga tgggctggtg ctacgtgggg
      721 gtagtgcaca ggttgcgcca ggcccagcgg cgccctcagc ggcagaaggc agtcagggtg
      781 gccatcctgg tgacaagcat cttcttcctc tgctggtcac cctaccacat cgtcatcttc
      841 ctggacaccc tggcgaggct gaaggccgtg gacaatacct gcaagctgaa tggctctctc
      901 cccgtggcca tcaccatgtg tgagttcctg ggcctggccc actgctgcct caaccccatg
      961 ctctacactt tcgccggcgt gaagttccgc agtgacctgt cgcggctcct gacgaagctg
     1021 ggctgtaccg gccctgcctc cctgtgccag ctcttcccta gctggcgcag gagcagtctc
     1081 tctgagtcag agaatgccac ctctctcacc acgttcgagg gcaggggaag tcttctaaca
     1141 tgcggggacg tggaggaaaa tcccggcccc atgacttcga aagtttatga tccagaacaa
     1201 aggaaacgga tgataactgg tccgcagtgg tgggccagat gtaaacaaat gaatgttctt
     1261 gattcattta ttaattatta tgattcagaa aaacatgcag aaaatgctgt tattttttta
     1321 catggtaacg cggcctcttc ttatttatgg cgacatgttg tgccacatat tgagccagta
     1381 gcgcggtgta ttataccaga tcttattggt atgggcaaat caggcaaatc tggtaatggt
     1441 tcttataggt tacttgatca ttacaaatat cttactgcat ggtttgaact tcttaattta
     1501 ccaaagaaga tcatttttgt cggccatgat tggggtgctt gtttggcatt tcattatagc
     1561 tatgagcatc aagataagat caaagcaata gttcacgctg aaagtgtagt agatgtgatt
     1621 gaatcatggg atgaatggcc tgatattgaa gaagatattg cgttgatcaa atctgaagaa
     1681 ggagaaaaaa tggttttgga gaataacttc ttcgtggaaa ccatgttgcc atcaaaaatc
     1741 atgagaaagt tagaaccaga agaatttgca gcatatcttg aaccattcaa agagaaaggt
     1801 gaagttcgtc gtccaacatt atcatggcct cgtgaaatcc cgttagtaaa aggtggtaaa
     1861 cctgacgttg tacaaattgt taggaattat aatgcttatc tacgtgcaag tgatgattta
     1921 ccaaaaatgt ttattgaatc ggatccagga ttcttttcca atgctattgt tgaaggcgcc
     1981 aagaagtttc ctaatactga atttgtcaaa gtaaaaggtc ttcatttttc gcaagaagat
     2041 gcacctgatg aaatgggaaa atatatcaaa tcgttcgttg agcgagttct caaaaatgaa
     2101 caataagggg accactttgt acaagaaagc tgggtgcccc tctccctccc ccccccctaa
     2161 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     2221 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     2281 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     2341 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     2401 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     2461 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     2521 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     2581 accccattgt atgggatctg atctggggcc tcggtacaca tgctttacat gtgtttagtc
     2641 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     2701 acgatgataa tatggccaca accatggtga gcaagggcga ggagctgttc accggggtgg
     2761 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     2821 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     2881 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     2941 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     3001 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     3061 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     3121 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     3181 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     3241 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3301 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     3361 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     3421 gcatggacga gctgtacaag tga
//