LOCUS       BBa_K1994015             176 bp    DNA     linear       17-May-2024
DEFINITION  sgRNA with 5' golden gate adapter and COM protein binding site
ACCESSION   BBa_K1994015
VERSION     BBa_K1994015.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=20nt golden gate adapter
     stem_loop       21..62
                     /label=dCas9 handle
     stem_loop       63..96
                     /label=truncated S. pyrogenes terminator
     misc_feature    97..106
                     /label=10bp linker 
     stem_loop       107..125
                     /label=COM handle
     misc_feature    126..135
                     /label=10bp linker
     stem_loop       136..176
                     /label=aspA terminator
ORIGIN
        1 agagaccact ttcggtctct gttttagagc tagaaatagc aagttaaaat aaggctagtc
       61 cgttatcaac ttgaaaaagt ggcaccgagt cggtgcacca ataccactga atgcctgcga
      121 gcatcaccaa taccaacaag aaaaaaggca cgtcatctga cgtgcctttt ttattt
//