LOCUS       BBa_K1994016             172 bp    DNA     linear       17-May-2024
DEFINITION  sgRNA with 5' golden gate adapter and MS2 coat protein binding site
ACCESSION   BBa_K1994016
VERSION     BBa_K1994016.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=20nt golden gate adapter
     stem_loop       21..62
                     /label=dCas9 handle
     stem_loop       63..96
                     /label=truncated S. pyrogenes terminator
     misc_feature    97..101
                     /label=5bp linker
     stem_loop       102..126
                     /label=MS2 handle
     misc_feature    127..131
                     /label=5bp linker
     misc_feature    132..172
                     /label=aspA terminator
ORIGIN
        1 agagaccact ttcggtctct gttttagagc tagaaatagc aagttaaaat aaggctagtc
       61 cgttatcaac ttgaaaaagt ggcaccgagt cggtgcacaa agcgcacatg aggattaccc
      121 atgtgcaccc aacaagaaaa aaggcacgtc atctgacgtg ccttttttat tt
//