LOCUS       BBa_K200019             1946 bp    DNA     linear       21-May-2024
DEFINITION  pLacI+RBS+RFP+TT+pCstA+RBS+GFP+TT
ACCESSION   BBa_K200019
VERSION     BBa_K200019.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..756
                     /label=mrfp1
     misc_feature    763..787
                     /label=Help:Barcodes
     CDS             82..762
                     /label=BBa_E1010
     stem_loop       807..850
                     /label=stem_loop
     misc_feature    796..875
                     /label=BBa_B0010
     terminator      796..875
                     /label=BBa_B0010
     stem_loop       891..910
                     /label=T7 TE
     misc_feature    917..917
                     /label=stop
     polyA_site      911..924
                     /label=polya
     misc_feature    884..924
                     /label=BBa_B0012
     terminator      884..924
                     /label=BBa_B0012
     misc_feature    64..924
                     /label=BBa_I13507
     misc_feature    1..924
                     /label=BBa_J5526
     misc_feature    933..974
                     /label=cAMP receptor protein binding site
     misc_feature    1033..1063
                     /label=RNA polymerase binding site
     promoter        933..1063
                     /label=BBa_K118011
     misc_feature    1076..1079
                     /label=conserved
     RBS             1072..1083
                     /label=BBa_B0034
     CDS             1090..1809
                     /label=GFP protein
     CDS             1090..1809
                     /label=BBa_E0040
     stem_loop       1829..1872
                     /label=stem_loop
     misc_feature    1818..1897
                     /label=BBa_B0010
     terminator      1818..1897
                     /label=BBa_B0010
     stem_loop       1913..1932
                     /label=T7 TE
     misc_feature    1939..1939
                     /label=stop
     polyA_site      1933..1946
                     /label=polya
     misc_feature    1906..1946
                     /label=BBa_B0012
     terminator      1906..1946
                     /label=BBa_B0012
     misc_feature    933..1946
                     /label=BBa_K200018
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt
      121 ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt
      181 gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg
      241 ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt
      301 aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg
      361 gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg
      421 caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt
      481 ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa
      541 gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac
      601 gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac
      661 aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag
      721 tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt
      781 agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      841 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
      901 cgggtgggcc tttctgcgtt tatatactag agactcggtt aacggagtga tcgagttaac
      961 attgttaagt taaatattgg tttcaactcc gatttacatg gttgctgtgt tgttaaattg
     1021 tacaaagatg ttatagaaac aaaatgtaac atctctatgg acatactaga gaaagaggag
     1081 aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
     1141 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
     1201 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
     1261 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat
     1321 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact
     1381 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat
     1441 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
     1501 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
     1561 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa
     1621 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
     1681 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
     1741 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac
     1801 aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1861 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1921 ttcgggtggg cctttctgcg tttata
//