BBa_K2008008
1
BBa_K2008008
pVeg->sec-TD1 modular secretion platform with ThrC homology
2016-10-16T11:00:00Z
2016-10-18T06:46:47Z
The pVeg promoter, sec secretion tag as well as the 125-bp AmyE regions of homology B. subtilis genome. TD1 is a transdermal tag first entered into the registry by the USTC_China iGEM team in 2013.
This part is meant to be used as a way to integrate any desired insert into the B. subtilis chromosome, which will then be secreted and possess the ability to diffuse transdermally through human skin.
The integration cassette contains the constitutive B. subtilis promoter pVeg (BBa_K143012) and a strong B. subtilis RBS (BBa_K780001), as well as a B. subtilis secretory signal peptide sequence to allow for secretion of the desired protein product directly into surrounding media. A transdermal tag (TD1, BBa_K1074000) is included to allow for diffusion of the protein product across the skin.
The sequence contains two internal restriction enzyme sites: one for BamHI and one for XmaI. These sites allow for the directional cloning of a desired insert.
Note: This part is a composite part, not a basic part, but it could not be added as composite at the time of entry.
false
false
_2475_
30207
30207
9
true
Addition of the AmyE homology regions are derived from B. subtilis 168 genomic DNA. Internal BamHI and XmaI restriction sites are present to allow for directional cloning of any desired insert. A secretory tag is present to allow for secretion of said insert, and a transdermal tag is present to allow the desired protein product to be secreted.
false
Rachelle Varga
annotation2515393
1
BBa_K1074000
range2515393
1
277
309
annotation2515395
1
BamHI site
range2515395
1
313
318
annotation2515397
1
3
range2515397
1
337
461
annotation2515388
1
BBa_K143012
range2515388
1
68
164
annotation2515386
1
5
range2515386
1
1
67
annotation2515391
1
Sec tag
range2515391
1
190
276
annotation2515396
1
XmaI site
range2515396
1
325
330
annotation2515390
1
BBa_K780001
range2515390
1
165
180
BBa_K2008008_sequence
1
tgcagcccgtgctaacatgaaatgcattgtcatcatcccgaacggaaaaattgcatttggaaaactcaattttgtcaaaataattttattgacaacgtcttattaacgttgatataatttaaattttatttgacaaaaatgggctcgtgttgtacaataaatgtatattaagaggaggagatatatataatgaatatcaagaagttcgcaaaacaggcgacagtcctgacctttaccaccgccctcttggcagggggggcgacccaggcattcgctgcttgttcttcttccccatctaagcattgtggtataggatcctaataacccgggtaataatgcagaaccaggttcttgcgcgtctatcgcaggagtgctgaaacaggtgaaatccggagaaattccgaaaggcagcaaggtcgtagctgtgttaacaggaaacggactgaaagatccgaacacag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z