BBa_K2010004 1 BBa_K2010004 sfGFP + PETase (PET-degrading enzyme, origin I. sakaiensis) 2016-06-20T11:00:00Z 2016-10-08T01:16:39Z This sequence is publicly known and was privately modified. This His-tag is a sequence that allows a conjunct protein to be purified. The tag is composed of repetitions of histidines. false false _2477_ 30331 30331 9 false The DNA sequence has been optimized for E. coli K12 and modified to facilitate gBlock synthesis, by avoiding repeated sequences. false William Cho annotation2483577 1 Linker range2483577 1 779 793 annotation2483582 1 Stop Codon range2483582 1 1718 1723 annotation2483580 1 His-Tag range2483580 1 1700 1717 annotation2483576 1 sfGFP range2483576 1 65 778 annotation2483579 1 GGS Linker range2483579 1 1664 1699 annotation2483575 1 RBS 34 range2483575 1 47 58 annotation2483581 1 Start Codon range2483581 1 65 67 annotation2483578 1 PETase range2483578 1 794 1663 annotation2480263 1 T7 Promoter range2480263 1 1 46 BBa_K2010004_sequence 1 taatacgactcactatagggaatacaagctacttgttctttttgcaaaagaggagaaatactagatgtcgaaaggggaggaattatttacaggtgtagtgccgattttggttgagctggacggcgatgtaaatggtcacaaattctccgtgcgtggagagggtgaaggcgatgcgacaaacggtaagttaacactgaaatttatctgcacaactggtaaattaccagttccttggcccacattggtcactacccttacatatggagtacagtgctttagccgctaccctgatcatatgaaacgccatgacttctttaagtcagcgatgcccgagggctatgtacaagagcgtaccatctcgtttaaggacgacgggacttataagacacgcgcggaagtaaagttcgaaggcgacactttagttaatcgtatcgaactgaagggtatcgacttcaaggaagatgggaacattttgggccacaaattagaatacaatttcaacagtcataacgtttatatcacggctgataagcagaagaatgggattaaagctaattttaagatccgtcataacgttgaggacggcagcgttcagctggcagaccattatcagcaaaacacgcccattggagacggtccggttcttctgccggacaaccactacttgtctacacagagcgtcttgtcgaaggacccaaatgagaagcgtgaccatatggttcttctggaatttgtcactgccgccggcatcacacacggcatggacgaattatataaagggtccaagggtccgatgaatttcccccgtgccagtcgtctgatgcaagctgccgtattgggcgggcttatggccgtttccgcagccgcgactgctcagacgaatccatatgcacgtggacctaaccccacagcagcgtcattagaagcgtcggcggggcctttcactgtccgcagtttcacggtgagccgtccgtcaggctatggagcgggaaccgtttattatcccaccaacgcaggtggcacggttggtgcaatcgctattgttcccgggtacacagcccgccagtcaagcatcaaatggtgggggcctcgtctggcaagtcatggctttgttgtcattactatcgacacaaacagtacgcttgaccaaccgagttcgcgctcttcccagcagatggccgcccttcgtcaagtagcatccttaaacggcacgagttcgtctccaatctatggaaaggtggacaccgcacgcatgggggtaatggggtggtctatggggggtggggggtcacttatcagtgcagcgaataacccgagtttaaaggcagcggccccccaggccccatgggattcgtctacaaatttctcatctgttaccgtcccgactcttattttcgcatgcgagaatgatagcattgcacctgttaatagttctgctttaccaatctacgactcgatgagccgtaatgcaaaacaatttctggaaatcaatggcgggtcgcactcttgtgcgaacagcggtaactcgaatcaagctttgatcgggaaaaagggtgtagcatggatgaagcgttttatggacaacgatactcgctacagcactttcgcttgtgagaacccgaatagtactcgcgtaagcgattttcgcaccgctaactgctccggggggtcgggagggtcagggggatcaggaggttcgcatcaccatcaccatcattagtag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z