LOCUS       BBa_K2010005            1789 bp    DNA     linear       14-May-2024
DEFINITION  ompT + sfGFP + PETase (PET-degrading enzyme, origin I. sakaiensis)
ACCESSION   BBa_K2010005
VERSION     BBa_K2010005.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=T7 Promoter
     RBS             47..58
                     /label=RBS 34
     misc_feature    65..124
                     /label=ompT Tag
     misc_feature    125..130
                     /label=Linker
     CDS             131..844
                     /label=sfGFP
     misc_feature    845..859
                     /label=Linker
     CDS             860..1729
                     /label=PETase
     misc_feature    1730..1765
                     /label=Linker
     misc_feature    1766..1783
                     /label=His-Tag
     misc_feature    1784..1789
                     /label=Stop Codon
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcaaaag aggagaaata
       61 ctagatgcgt gcaaaattat tgggtatcgt gcttaccacg cctattgcga ttagctcatt
      121 tgcaggatct atgtcgaaag gggaggaatt atttacaggt gtagtgccga ttttggttga
      181 gctggacggc gatgtaaatg gtcacaaatt ctccgtgcgt ggagagggtg aaggcgatgc
      241 gacaaacggt aagttaacac tgaaatttat ctgcacaact ggtaaattac cagttccttg
      301 gcccacattg gtcactaccc ttacatatgg agtacagtgc tttagccgct accctgatca
      361 tatgaaacgc catgacttct ttaagtcagc gatgcccgag ggctatgtac aagagcgtac
      421 catctcgttt aaggacgacg ggacttataa gacacgcgcg gaagtaaagt tcgaaggcga
      481 cactttagtt aatcgtatcg aactgaaggg tatcgacttc aaggaagatg ggaacatttt
      541 gggccacaaa ttagaataca atttcaacag tcataacgtt tatatcacgg ctgataagca
      601 gaagaatggg attaaagcta attttaagat ccgtcataac gttgaggacg gcagcgttca
      661 gctggcagac cattatcagc aaaacacgcc cattggagac ggtccggttc ttctgccgga
      721 caaccactac ttgtctacac agagcgtctt gtcgaaggac ccaaatgaga agcgtgacca
      781 tatggttctt ctggaatttg tcactgccgc cggcatcaca cacggcatgg acgaattata
      841 taaagggtcc aagggtccga tgaatttccc ccgtgccagt cgtctgatgc aagctgccgt
      901 attgggcggg cttatggccg tttccgcagc cgcgactgct cagacgaatc catatgcacg
      961 tggacctaac cccacagcag cgtcattaga agcgtcggcg gggcctttca ctgtccgcag
     1021 tttcacggtg agccgtccgt caggctatgg agcgggaacc gtttattatc ccaccaacgc
     1081 aggtggcacg gttggtgcaa tcgctattgt tcccgggtac acagcccgcc agtcaagcat
     1141 caaatggtgg gggcctcgtc tggcaagtca tggctttgtt gtcattacta tcgacacaaa
     1201 cagtacgctt gaccaaccga gttcgcgctc ttcccagcag atggccgccc ttcgtcaagt
     1261 agcatcctta aacggcacga gttcgtctcc aatctatgga aaggtggaca ccgcacgcat
     1321 gggggtaatg gggtggtcta tggggggtgg ggggtcactt atcagtgcag cgaataaccc
     1381 gagtttaaag gcagcggccc cccaggcccc atgggattcg tctacaaatt tctcatctgt
     1441 taccgtcccg actcttattt tcgcatgcga gaatgatagc attgcacctg ttaatagttc
     1501 tgctttacca atctacgact cgatgagccg taatgcaaaa caatttctgg aaatcaatgg
     1561 cgggtcgcac tcttgtgcga acagcggtaa ctcgaatcaa gctttgatcg ggaaaaaggg
     1621 tgtagcatgg atgaagcgtt ttatggacaa cgatactcgc tacagcactt tcgcttgtga
     1681 gaacccgaat agtactcgcg taagcgattt tcgcaccgct aactgctccg gggggtcggg
     1741 agggtcaggg ggatcaggag gttcgcatca ccatcaccat cattagtag
//