BBa_K2012000
1
BBa_K2012000
c-di-GMP tandem riboswitche bc3-5
2016-08-04T11:00:00Z
2016-10-09T01:16:18Z
From Bacillus thuringiensis subsp. chinensis CT-43
In the CT-43 genome19,20 (GenBank accession: NC_017208.1), five type I c-di-GMP riboswitches named Bc1, Bc2, Bc3, Bc4 and Bc5 RNA (Supplementary Tables S1???S5) were annotated in the Rfam database (http://rfam.sanger.ac.uk/search).
Zhou, H., et al. (2016). "Characterization of a natural triple-tandem c-di-GMP riboswitch and application of the riboswitch-based dual-fluorescence reporter." Sci Rep 6: 20871.
Three complete c-di-GMP riboswitches (Bc3, Bc4 and Bc5 RNA) with similar structures, which are arranged in tandem to constitute a triple-tandem (Bc3-5 RNA) riboswitch in the 5′-UTR of the cspABCDE mRNA in Bacillus thuringiensis subsp. chinensis CT-43.
false
false
_2479_
25212
25212
9
false
We designed the sequence of bc3-5 by add perfix and suffix which are belong to BioBrick RFC 10.
false
Pan Chu
BBa_K2012011
1
BBa_K2012011
J23117-Bc345
2016-09-14T11:00:00Z
2016-09-15T12:16:33Z
J23117 form iGEM parts kit
Bc345: see BBa_K2012001
Tandem rioswitches bc345 are located in the promoter J23117 upstream. It can control gene expression. There are low leakage
expression of gene which you interest in E.coli whose concentration of c-di-GMP is in a normal level. When cell is induced expression gene which can catalyze c-di-GMP from GTP, the tandem riboswitches would trans to open form and express its downstream gene.
false
false
_2479_
25212
25212
9
false
The bc3 riboswitch is apart of the tandem riboswitch bc3-5. We analysed the sequence of bc3-5 and its scendary structure, found linkers between three complete riboswitches. Using PCR amplify the bc3 sequence which is similar with bc4 and bc5 with prefix and suffix, whereas the secondary structure of bc3 is different from other riboswitches'.
false
Pan Chu
component2483440
1
BBa_J23117
component2483441
1
BBa_K2012000
annotation2483440
1
BBa_J23117
range2483440
1
1
35
annotation2483441
1
BBa_K2012000
range2483441
1
44
528
BBa_J23117
1
BBa_J23117
constitutive promoter family member
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
Later
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_J23117_sequence
1
ttgacagctagctcagtcctagggattgtgctagc
BBa_K2012011_sequence
1
ttgacagctagctcagtcctagggattgtgctagctactagagccacgataaataaatacctatttttggcacactattcgaaaggataggtcgcaaagctaagagtctaaagtaatgaaaattactatgatagtctggttgcagtttggattttcacacatagttgtatgtatgaaaatcgaagaggcaaccggattttttattgtctcaaaaagaaaaaataaatgggcacactattcgaaaggataggtcgcaaagctaagagtctaaggtaatgaaaattactatgatagtctggttgcagtttggattttcacacatgttgtgatgtatgaaaatcgaaaaggcaaccagattttttattgtctcaaaaagaaaaaataaatgggcacactgttcgaaaggataggtcgcaaagctaagagtctaaggtaatgaaaattactatgatagtctggttgcagtttggattttcacacatgttgtgatgtatgaaaatcgaaaaggcaaccaggctttttattttg
BBa_K2012000_sequence
1
ccacgataaataaatacctatttttggcacactattcgaaaggataggtcgcaaagctaagagtctaaagtaatgaaaattactatgatagtctggttgcagtttggattttcacacatagttgtatgtatgaaaatcgaagaggcaaccggattttttattgtctcaaaaagaaaaaataaatgggcacactattcgaaaggataggtcgcaaagctaagagtctaaggtaatgaaaattactatgatagtctggttgcagtttggattttcacacatgttgtgatgtatgaaaatcgaaaaggcaaccagattttttattgtctcaaaaagaaaaaataaatgggcacactgttcgaaaggataggtcgcaaagctaagagtctaaggtaatgaaaattactatgatagtctggttgcagtttggattttcacacatgttgtgatgtatgaaaatcgaaaaggcaaccaggctttttattttg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z