BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_Z0251
1
BBa_Z0251
T7 strong promoter
2005-04-04T11:00:00Z
2015-05-08T01:14:55Z
bacteriophage T7
Promoter for T7 RNA polymerase
false
false
_10_
0
250
10
Not in stock
false
false
T7.2
annotation1476333
1
consensus sequence
range1476333
1
6
28
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K2027073
1
BBa_K2027073
(pMS-AFa-DEa) pAKP444 RBS 1 extraction
2016-10-24T11:00:00Z
2016-10-25T07:42:54Z
Sequence was sourced from Turk SC, Kloosterman WP, Ninaber DK, et al. Metabolic Engineering toward Sustainable Production of Nylon-6. ACS Synth Biol. 2016;5(1):65-73. (http://pubs.acs.org/doi/full/10.1021/acssynbio.5b00129)
(pMS-AFa-DEa) pAKP444 RBS 1 extraction Ribosome binding site extraction out of pAKP444 plasmid
false
false
_2494_
31619
31619
9
false
No alterations were made to the sequence.
false
Gordon Sun
BBa_K2027053
1
BBa_K2027053
<i>H. Brasiliensis</i> SRPP cDNA (codon Optimized)
2016-10-23T11:00:00Z
2016-10-24T02:36:47Z
Sequence was sourced from Oh SK, Kang H, Shin DH, et al. Isolation, characterization, and functional analysis of a novel cDNA clone encoding a small rubber particle protein from Hevea brasiliensis. J Biol Chem. 1999;274(24):17132-8. (http://www.ncbi.nlm.nih.gov/nuccore/AF051317)
This sequence contains the gene for a small rubber particle protein (SRPP) from the rubber tree Hevea brasiliensis. This coenzyme serves as an activator for rubber transferases/prenyltransferases in H. brasiliensis. This protein is involved in rubber elongation.
Characteristics:
??? 612bp ORF
??? 63-bp 59-UTR
??? 181-bp 39-UTR
??? 18bp poly A tail
??? Localized in the cytoplasm
false
false
_2494_
31619
31619
9
false
This sequence has been codon optimized for transfection into E. coli.
Mutation A486T was induced to remove a restriction site.
A FLAG-Lumio tag can be appended to the sequence to allow for visualization and/or purification.
false
Gordon Sun
BBa_K2027052
1
BBa_K2027052
Flag-Lumio-HisTag
2016-10-23T11:00:00Z
2016-10-24T01:41:34Z
See BBa_K2027000
Scar sequence between T7 Promoter and RBS: TACTAGAG
false
false
_2494_
29123
29123
9
false
Necessary spacing
false
Michael Becich
BBa_K2027074
1
BBa_K2027074
(pMS-AFa-DEa) pAKP444 RBS 2 extraction
2016-10-24T11:00:00Z
2016-10-25T08:13:34Z
Sequence sourced from Turk SC, Kloosterman WP, Ninaber DK, et al. Metabolic Engineering toward Sustainable Production of Nylon-6. ACS Synth Biol. 2016;5(1):65-73. (http://pubs.acs.org/doi/full/10.1021/acssynbio.5b00129)
(pMS-AFa-DEa) pAKP444 RBS 2extraction ribosome binding site extracted from plasmid pAKP444.
false
false
_2494_
31619
31619
9
false
No alterations were made to the sequence.
false
Gordon Sun
BBa_K2027054
1
BBa_K2027054
<i>H. Brasiliensis</i> HRT1 cDNA (codon Optimized)
2016-10-23T11:00:00Z
2016-10-24T02:36:40Z
Sourced from Asawatreratanakul K, Zhang YW, Wititsuwannakul D, et al. Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis. A key factor participating in natural rubber biosynthesis. Eur J Biochem. 2003;270(23):4671-80. (http://www.ncbi.nlm.nih.gov/nuccore/AB061234?report=GenBank)
This sequence contains the <i>Hevea brasiliensis</i>??HRT1??mRNA for cis-prenyltransferase, complete cds. The sequence has been codon optimized for expression in <i>E. coli</i>. HRT1 is a cis-prenyltransferase and is responsible for initialization and elongation of polyisoprene polymers. It takes in IPP or DMAPP and polymerizes it to a polyisoprene chain.
false
false
_2494_
31619
31619
9
false
This sequence has been codon optimized for expression in <i>E. coli</i>.
No other modifications have been made to the sequence.
false
Gordon Sun
BBa_K2027039
1
Latex Oper
Rubber Transferases + Small Rubber Particle Protein Cassette
2016-10-12T11:00:00Z
2016-10-25T09:32:42Z
TBA
TBA
false
false
_2494_
29123
29123
9
true
TBA
false
Gordon Sun, Taylor Sihavong, Michael Becich
component2530224
1
BBa_B0010
component2530216
1
BBa_K2027053
component2530223
1
BBa_K2027052
component2530218
1
BBa_K2027073
component2530226
1
BBa_B0012
component2530220
1
BBa_K2027052
component2530213
1
BBa_Z0251
component2530219
1
BBa_K2027054
component2530217
1
BBa_K2027052
component2530222
1
BBa_K2027055
component2530221
1
BBa_K2027074
component2530215
1
BBa_B0034
annotation2530218
1
BBa_K2027073
range2530218
1
762
789
annotation2530223
1
BBa_K2027052
range2530223
1
2646
2717
annotation2530215
1
BBa_B0034
range2530215
1
44
55
annotation2530213
1
BBa_Z0251
range2530213
1
1
35
annotation2530221
1
BBa_K2027074
range2530221
1
1754
1779
annotation2530216
1
BBa_K2027053
range2530216
1
62
673
annotation2530226
1
BBa_B0012
range2530226
1
2814
2854
annotation2530219
1
BBa_K2027054
range2530219
1
796
1665
annotation2530217
1
BBa_K2027052
range2530217
1
682
753
annotation2530222
1
BBa_K2027055
range2530222
1
1786
2637
annotation2530220
1
BBa_K2027052
range2530220
1
1674
1745
annotation2530224
1
BBa_B0010
range2530224
1
2726
2805
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_K2027055
1
BBa_K2027055
<i>H. Brasiliensis</i> HRT2 cis-prenyltransferase
2016-10-23T11:00:00Z
2016-10-24T02:36:28Z
Sequence was sourced from Asawatreratanakul K, Zhang YW, Wititsuwannakul D, et al. Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis. A key factor participating in natural rubber biosynthesis. Eur J Biochem. 2003;270(23):4671-80. (http://www.ncbi.nlm.nih.gov/nuccore/AB064661?report=GenBank)
This sequence codes for a cis-prenyltransferase responsible for polymerization of IPP and DMAPP into polyisoprene compounds. This sequence is from the rubber tree, <i>Hevea brasiliensis</i>
false
false
_2494_
31619
31619
9
false
This sequence has been acquired from <i>H. brasiliensis</i> and codon optimized for transfection into <i>E. coli</i>.
No other modifications have been made to the sequence.
false
Gordon Sun
BBa_K2027055_sequence
1
atggaactttacaacggcgaacgtccatccgtgttccgtcttcttggcaaatacatgcgtaaagggctgtattctatcttaacgcagggtcctattcctacccatattgcctttattttggacggaaacgggcgcttcgcgaagaagcacaaactgcccgaaggaggaggccacaaagcgggatttttggccttacttaacgtcttaacttactgttatgagttaggagttaaatatgctacaatttacgccttctcgattgataatttccgccgtaaaccgcacgaggtacagtatgttatgaacttgatgttagagaaaatcgagggcatgatcatggaagaatcaattattaacgcctatgatatttgcgtgcgttttgtggggaatcttaaacttttggatgagccattgaaaacggcggcagataagatcatgcgtgcgacagccaaaaattctaaatttgttctgctgcttgcagtttgctatacatcgacagacgagatcgtacacgctgtggaagaatcttctaaggacaagctgaaatcggatgagatttgcaatgacggcaacggtgactgcgtaattaagattgaagagatggagccatattctgaaattaagctggttgagttggagcgcaatacctacatcaacccgtatcctgatgtcttaattcgcacaagcggggaaacgcgcttgagcaactacctgttgtggcaaacaactaactgtatcctttattctccacatgctttatggcctgaaattggcttgcgccatgtcgtctgggcggttattaactgccagcgtcactacagctatttggagaaacataaggaatacctgaaa
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K2027074_sequence
1
taataaggtaccaggaggaaactata
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K2027073_sequence
1
taataaggtaccaggaggaattaaccat
BBa_K2027039_sequence
1
gaaattaatacgactcactatagggagaccacaactactagagaaagaggagaaatactagatggccgaagaggtagaggaagagcgcctgaaatatctggactttgtacgcgcagctggagtctatgcggttgattcattctctacgttgtacttgtatgccaaggacatttcaggcccattgaaacctggggtagacaccatcgagaatgtcgtcaagacggtagttaccccggtgtactatatccctttagaagcagttaagtttgtcgacaaaacggttgatgttagtgttacgagtttagacggcgttgtgccgccagtaattaagcaagtatcggcacagacctacagcgtcgcgcaggatgctccccgcattgtgttagatgtcgcttcttctgtttttaacacgggcgttcaggaaggagcaaaagcgctgtacgcaaaccttgagccaaaggccgaacagtacgcggttatcacatggcgcgcacttaataaacttcctcttgtcccacaagttgcgaatgtcgttgtaccgactgctgtttattttagcgaaaaatataacgatgttgtacgtggcactaccgagcaaggctaccgtgttagctcgtaccttcctctgttacctacggaaaaaatcacgaaggtattcggagacgaggcatcatactagaggactacaaagacgatgacgacaagtgttgtcctggctgttgcggtgctggtggccatcatcaccatcaccattactagagtaataaggtaccaggaggaattaaccattactagatggaattgtacaacggggaacgcccctcggtttttcgtttgttaggaaagtacatgcgtaaagggttatactctattttaacacagggaccaattccaacgcacatcgcctttatcctggacggaaatcgtcgttttgcaaaaaaacacaagttgcccgaaggcggtggccacaaggcgggttttcttgcccttctgaatgtcctgacatattgttatgagctgggggtaaagtatgcaactatttacgccttttccattgacaactttcgccgtaaacctcacgaagtccaatacgtcatggatctgatgttggaaaagatcgaaggcatgattatggaagagtcgatcatcaacgcttatgacatttgtgtccgctttgtcggtaatttaaaattgttatccgagccggttaagacagccgccgacaaaatcatgcgcgctacagccaacaattcaaaatgtgtccttttaatcgcggtatgttacacgtccacagacgaaattgtacatgccgtagaagaaagttcagaacttaattcgaacgaggtatgcaacaatcaagagttagaggaggcaaatgcgactgggtcgggcaccgtgattcaaatcgaaaacatggaatcttatagcggaattaaacttgtcgatttggagaaaaacacctatatcaacccttaccctgatgtacttattcgtacatcaggcgagacacgtttatcaaattacttgctgtggcagacgactaattgcattttatactccccgcacgctctttggccggagattgggctgcgtcacgtcgtctgggctgtgattaattttcagcgccactactcctacttggagaaacataaggagtatcttaaatactagaggactacaaagacgatgacgacaagtgttgtcctggctgttgcggtgctggtggccatcatcaccatcaccattactagagtaataaggtaccaggaggaaactatatactagatggaactttacaacggcgaacgtccatccgtgttccgtcttcttggcaaatacatgcgtaaagggctgtattctatcttaacgcagggtcctattcctacccatattgcctttattttggacggaaacgggcgcttcgcgaagaagcacaaactgcccgaaggaggaggccacaaagcgggatttttggccttacttaacgtcttaacttactgttatgagttaggagttaaatatgctacaatttacgccttctcgattgataatttccgccgtaaaccgcacgaggtacagtatgttatgaacttgatgttagagaaaatcgagggcatgatcatggaagaatcaattattaacgcctatgatatttgcgtgcgttttgtggggaatcttaaacttttggatgagccattgaaaacggcggcagataagatcatgcgtgcgacagccaaaaattctaaatttgttctgctgcttgcagtttgctatacatcgacagacgagatcgtacacgctgtggaagaatcttctaaggacaagctgaaatcggatgagatttgcaatgacggcaacggtgactgcgtaattaagattgaagagatggagccatattctgaaattaagctggttgagttggagcgcaatacctacatcaacccgtatcctgatgtcttaattcgcacaagcggggaaacgcgcttgagcaactacctgttgtggcaaacaactaactgtatcctttattctccacatgctttatggcctgaaattggcttgcgccatgtcgtctgggcggttattaactgccagcgtcactacagctatttggagaaacataaggaatacctgaaatactagaggactacaaagacgatgacgacaagtgttgtcctggctgttgcggtgctggtggccatcatcaccatcaccattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_Z0251_sequence
1
gaaattaatacgactcactatagggagaccacaac
BBa_K2027053_sequence
1
atggccgaagaggtagaggaagagcgcctgaaatatctggactttgtacgcgcagctggagtctatgcggttgattcattctctacgttgtacttgtatgccaaggacatttcaggcccattgaaacctggggtagacaccatcgagaatgtcgtcaagacggtagttaccccggtgtactatatccctttagaagcagttaagtttgtcgacaaaacggttgatgttagtgttacgagtttagacggcgttgtgccgccagtaattaagcaagtatcggcacagacctacagcgtcgcgcaggatgctccccgcattgtgttagatgtcgcttcttctgtttttaacacgggcgttcaggaaggagcaaaagcgctgtacgcaaaccttgagccaaaggccgaacagtacgcggttatcacatggcgcgcacttaataaacttcctcttgtcccacaagttgcgaatgtcgttgtaccgactgctgtttattttagcgaaaaatataacgatgttgtacgtggcactaccgagcaaggctaccgtgttagctcgtaccttcctctgttacctacggaaaaaatcacgaaggtattcggagacgaggcatca
BBa_K2027054_sequence
1
atggaattgtacaacggggaacgcccctcggtttttcgtttgttaggaaagtacatgcgtaaagggttatactctattttaacacagggaccaattccaacgcacatcgcctttatcctggacggaaatcgtcgttttgcaaaaaaacacaagttgcccgaaggcggtggccacaaggcgggttttcttgcccttctgaatgtcctgacatattgttatgagctgggggtaaagtatgcaactatttacgccttttccattgacaactttcgccgtaaacctcacgaagtccaatacgtcatggatctgatgttggaaaagatcgaaggcatgattatggaagagtcgatcatcaacgcttatgacatttgtgtccgctttgtcggtaatttaaaattgttatccgagccggttaagacagccgccgacaaaatcatgcgcgctacagccaacaattcaaaatgtgtccttttaatcgcggtatgttacacgtccacagacgaaattgtacatgccgtagaagaaagttcagaacttaattcgaacgaggtatgcaacaatcaagagttagaggaggcaaatgcgactgggtcgggcaccgtgattcaaatcgaaaacatggaatcttatagcggaattaaacttgtcgatttggagaaaaacacctatatcaacccttaccctgatgtacttattcgtacatcaggcgagacacgtttatcaaattacttgctgtggcagacgactaattgcattttatactccccgcacgctctttggccggagattgggctgcgtcacgtcgtctgggctgtgattaattttcagcgccactactcctacttggagaaacataaggagtatcttaaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K2027052_sequence
1
gactacaaagacgatgacgacaagtgttgtcctggctgttgcggtgctggtggccatcatcaccatcaccat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z