BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_Z0251 1 BBa_Z0251 T7 strong promoter 2005-04-04T11:00:00Z 2015-05-08T01:14:55Z bacteriophage T7 Promoter for T7 RNA polymerase false false _10_ 0 250 10 Not in stock false false T7.2 annotation1476333 1 consensus sequence range1476333 1 6 28 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K2027073 1 BBa_K2027073 (pMS-AFa-DEa) pAKP444 RBS 1 extraction 2016-10-24T11:00:00Z 2016-10-25T07:42:54Z Sequence was sourced from Turk SC, Kloosterman WP, Ninaber DK, et al. Metabolic Engineering toward Sustainable Production of Nylon-6. ACS Synth Biol. 2016;5(1):65-73. (http://pubs.acs.org/doi/full/10.1021/acssynbio.5b00129) (pMS-AFa-DEa) pAKP444 RBS 1 extraction Ribosome binding site extraction out of pAKP444 plasmid false false _2494_ 31619 31619 9 false No alterations were made to the sequence. false Gordon Sun BBa_K2027053 1 BBa_K2027053 <i>H. Brasiliensis</i> SRPP cDNA (codon Optimized) 2016-10-23T11:00:00Z 2016-10-24T02:36:47Z Sequence was sourced from Oh SK, Kang H, Shin DH, et al. Isolation, characterization, and functional analysis of a novel cDNA clone encoding a small rubber particle protein from Hevea brasiliensis. J Biol Chem. 1999;274(24):17132-8. (http://www.ncbi.nlm.nih.gov/nuccore/AF051317) This sequence contains the gene for a small rubber particle protein (SRPP) from the rubber tree Hevea brasiliensis. This coenzyme serves as an activator for rubber transferases/prenyltransferases in H. brasiliensis. This protein is involved in rubber elongation. Characteristics: ??? 612bp ORF ??? 63-bp 59-UTR ??? 181-bp 39-UTR ??? 18bp poly A tail ??? Localized in the cytoplasm false false _2494_ 31619 31619 9 false This sequence has been codon optimized for transfection into E. coli. Mutation A486T was induced to remove a restriction site. A FLAG-Lumio tag can be appended to the sequence to allow for visualization and/or purification. false Gordon Sun BBa_K2027052 1 BBa_K2027052 Flag-Lumio-HisTag 2016-10-23T11:00:00Z 2016-10-24T01:41:34Z See BBa_K2027000 Scar sequence between T7 Promoter and RBS: TACTAGAG false false _2494_ 29123 29123 9 false Necessary spacing false Michael Becich BBa_K2027074 1 BBa_K2027074 (pMS-AFa-DEa) pAKP444 RBS 2 extraction 2016-10-24T11:00:00Z 2016-10-25T08:13:34Z Sequence sourced from Turk SC, Kloosterman WP, Ninaber DK, et al. Metabolic Engineering toward Sustainable Production of Nylon-6. ACS Synth Biol. 2016;5(1):65-73. (http://pubs.acs.org/doi/full/10.1021/acssynbio.5b00129) (pMS-AFa-DEa) pAKP444 RBS 2extraction ribosome binding site extracted from plasmid pAKP444. false false _2494_ 31619 31619 9 false No alterations were made to the sequence. false Gordon Sun BBa_K2027054 1 BBa_K2027054 <i>H. Brasiliensis</i> HRT1 cDNA (codon Optimized) 2016-10-23T11:00:00Z 2016-10-24T02:36:40Z Sourced from Asawatreratanakul K, Zhang YW, Wititsuwannakul D, et al. Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis. A key factor participating in natural rubber biosynthesis. Eur J Biochem. 2003;270(23):4671-80. (http://www.ncbi.nlm.nih.gov/nuccore/AB061234?report=GenBank) This sequence contains the <i>Hevea brasiliensis</i>??HRT1??mRNA for cis-prenyltransferase, complete cds. The sequence has been codon optimized for expression in <i>E. coli</i>. HRT1 is a cis-prenyltransferase and is responsible for initialization and elongation of polyisoprene polymers. It takes in IPP or DMAPP and polymerizes it to a polyisoprene chain. false false _2494_ 31619 31619 9 false This sequence has been codon optimized for expression in <i>E. coli</i>. No other modifications have been made to the sequence. false Gordon Sun BBa_K2027039 1 Latex Oper Rubber Transferases + Small Rubber Particle Protein Cassette 2016-10-12T11:00:00Z 2016-10-25T09:32:42Z TBA TBA false false _2494_ 29123 29123 9 true TBA false Gordon Sun, Taylor Sihavong, Michael Becich component2530224 1 BBa_B0010 component2530216 1 BBa_K2027053 component2530223 1 BBa_K2027052 component2530218 1 BBa_K2027073 component2530226 1 BBa_B0012 component2530220 1 BBa_K2027052 component2530213 1 BBa_Z0251 component2530219 1 BBa_K2027054 component2530217 1 BBa_K2027052 component2530222 1 BBa_K2027055 component2530221 1 BBa_K2027074 component2530215 1 BBa_B0034 annotation2530218 1 BBa_K2027073 range2530218 1 762 789 annotation2530223 1 BBa_K2027052 range2530223 1 2646 2717 annotation2530215 1 BBa_B0034 range2530215 1 44 55 annotation2530213 1 BBa_Z0251 range2530213 1 1 35 annotation2530221 1 BBa_K2027074 range2530221 1 1754 1779 annotation2530216 1 BBa_K2027053 range2530216 1 62 673 annotation2530226 1 BBa_B0012 range2530226 1 2814 2854 annotation2530219 1 BBa_K2027054 range2530219 1 796 1665 annotation2530217 1 BBa_K2027052 range2530217 1 682 753 annotation2530222 1 BBa_K2027055 range2530222 1 1786 2637 annotation2530220 1 BBa_K2027052 range2530220 1 1674 1745 annotation2530224 1 BBa_B0010 range2530224 1 2726 2805 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_K2027055 1 BBa_K2027055 <i>H. Brasiliensis</i> HRT2 cis-prenyltransferase 2016-10-23T11:00:00Z 2016-10-24T02:36:28Z Sequence was sourced from Asawatreratanakul K, Zhang YW, Wititsuwannakul D, et al. Molecular cloning, expression and characterization of cDNA encoding cis-prenyltransferases from Hevea brasiliensis. A key factor participating in natural rubber biosynthesis. Eur J Biochem. 2003;270(23):4671-80. (http://www.ncbi.nlm.nih.gov/nuccore/AB064661?report=GenBank) This sequence codes for a cis-prenyltransferase responsible for polymerization of IPP and DMAPP into polyisoprene compounds. This sequence is from the rubber tree, <i>Hevea brasiliensis</i> false false _2494_ 31619 31619 9 false This sequence has been acquired from <i>H. brasiliensis</i> and codon optimized for transfection into <i>E. coli</i>. No other modifications have been made to the sequence. false Gordon Sun BBa_K2027055_sequence 1 atggaactttacaacggcgaacgtccatccgtgttccgtcttcttggcaaatacatgcgtaaagggctgtattctatcttaacgcagggtcctattcctacccatattgcctttattttggacggaaacgggcgcttcgcgaagaagcacaaactgcccgaaggaggaggccacaaagcgggatttttggccttacttaacgtcttaacttactgttatgagttaggagttaaatatgctacaatttacgccttctcgattgataatttccgccgtaaaccgcacgaggtacagtatgttatgaacttgatgttagagaaaatcgagggcatgatcatggaagaatcaattattaacgcctatgatatttgcgtgcgttttgtggggaatcttaaacttttggatgagccattgaaaacggcggcagataagatcatgcgtgcgacagccaaaaattctaaatttgttctgctgcttgcagtttgctatacatcgacagacgagatcgtacacgctgtggaagaatcttctaaggacaagctgaaatcggatgagatttgcaatgacggcaacggtgactgcgtaattaagattgaagagatggagccatattctgaaattaagctggttgagttggagcgcaatacctacatcaacccgtatcctgatgtcttaattcgcacaagcggggaaacgcgcttgagcaactacctgttgtggcaaacaactaactgtatcctttattctccacatgctttatggcctgaaattggcttgcgccatgtcgtctgggcggttattaactgccagcgtcactacagctatttggagaaacataaggaatacctgaaa BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K2027074_sequence 1 taataaggtaccaggaggaaactata BBa_B0034_sequence 1 aaagaggagaaa BBa_K2027073_sequence 1 taataaggtaccaggaggaattaaccat BBa_K2027039_sequence 1 gaaattaatacgactcactatagggagaccacaactactagagaaagaggagaaatactagatggccgaagaggtagaggaagagcgcctgaaatatctggactttgtacgcgcagctggagtctatgcggttgattcattctctacgttgtacttgtatgccaaggacatttcaggcccattgaaacctggggtagacaccatcgagaatgtcgtcaagacggtagttaccccggtgtactatatccctttagaagcagttaagtttgtcgacaaaacggttgatgttagtgttacgagtttagacggcgttgtgccgccagtaattaagcaagtatcggcacagacctacagcgtcgcgcaggatgctccccgcattgtgttagatgtcgcttcttctgtttttaacacgggcgttcaggaaggagcaaaagcgctgtacgcaaaccttgagccaaaggccgaacagtacgcggttatcacatggcgcgcacttaataaacttcctcttgtcccacaagttgcgaatgtcgttgtaccgactgctgtttattttagcgaaaaatataacgatgttgtacgtggcactaccgagcaaggctaccgtgttagctcgtaccttcctctgttacctacggaaaaaatcacgaaggtattcggagacgaggcatcatactagaggactacaaagacgatgacgacaagtgttgtcctggctgttgcggtgctggtggccatcatcaccatcaccattactagagtaataaggtaccaggaggaattaaccattactagatggaattgtacaacggggaacgcccctcggtttttcgtttgttaggaaagtacatgcgtaaagggttatactctattttaacacagggaccaattccaacgcacatcgcctttatcctggacggaaatcgtcgttttgcaaaaaaacacaagttgcccgaaggcggtggccacaaggcgggttttcttgcccttctgaatgtcctgacatattgttatgagctgggggtaaagtatgcaactatttacgccttttccattgacaactttcgccgtaaacctcacgaagtccaatacgtcatggatctgatgttggaaaagatcgaaggcatgattatggaagagtcgatcatcaacgcttatgacatttgtgtccgctttgtcggtaatttaaaattgttatccgagccggttaagacagccgccgacaaaatcatgcgcgctacagccaacaattcaaaatgtgtccttttaatcgcggtatgttacacgtccacagacgaaattgtacatgccgtagaagaaagttcagaacttaattcgaacgaggtatgcaacaatcaagagttagaggaggcaaatgcgactgggtcgggcaccgtgattcaaatcgaaaacatggaatcttatagcggaattaaacttgtcgatttggagaaaaacacctatatcaacccttaccctgatgtacttattcgtacatcaggcgagacacgtttatcaaattacttgctgtggcagacgactaattgcattttatactccccgcacgctctttggccggagattgggctgcgtcacgtcgtctgggctgtgattaattttcagcgccactactcctacttggagaaacataaggagtatcttaaatactagaggactacaaagacgatgacgacaagtgttgtcctggctgttgcggtgctggtggccatcatcaccatcaccattactagagtaataaggtaccaggaggaaactatatactagatggaactttacaacggcgaacgtccatccgtgttccgtcttcttggcaaatacatgcgtaaagggctgtattctatcttaacgcagggtcctattcctacccatattgcctttattttggacggaaacgggcgcttcgcgaagaagcacaaactgcccgaaggaggaggccacaaagcgggatttttggccttacttaacgtcttaacttactgttatgagttaggagttaaatatgctacaatttacgccttctcgattgataatttccgccgtaaaccgcacgaggtacagtatgttatgaacttgatgttagagaaaatcgagggcatgatcatggaagaatcaattattaacgcctatgatatttgcgtgcgttttgtggggaatcttaaacttttggatgagccattgaaaacggcggcagataagatcatgcgtgcgacagccaaaaattctaaatttgttctgctgcttgcagtttgctatacatcgacagacgagatcgtacacgctgtggaagaatcttctaaggacaagctgaaatcggatgagatttgcaatgacggcaacggtgactgcgtaattaagattgaagagatggagccatattctgaaattaagctggttgagttggagcgcaatacctacatcaacccgtatcctgatgtcttaattcgcacaagcggggaaacgcgcttgagcaactacctgttgtggcaaacaactaactgtatcctttattctccacatgctttatggcctgaaattggcttgcgccatgtcgtctgggcggttattaactgccagcgtcactacagctatttggagaaacataaggaatacctgaaatactagaggactacaaagacgatgacgacaagtgttgtcctggctgttgcggtgctggtggccatcatcaccatcaccattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_Z0251_sequence 1 gaaattaatacgactcactatagggagaccacaac BBa_K2027053_sequence 1 atggccgaagaggtagaggaagagcgcctgaaatatctggactttgtacgcgcagctggagtctatgcggttgattcattctctacgttgtacttgtatgccaaggacatttcaggcccattgaaacctggggtagacaccatcgagaatgtcgtcaagacggtagttaccccggtgtactatatccctttagaagcagttaagtttgtcgacaaaacggttgatgttagtgttacgagtttagacggcgttgtgccgccagtaattaagcaagtatcggcacagacctacagcgtcgcgcaggatgctccccgcattgtgttagatgtcgcttcttctgtttttaacacgggcgttcaggaaggagcaaaagcgctgtacgcaaaccttgagccaaaggccgaacagtacgcggttatcacatggcgcgcacttaataaacttcctcttgtcccacaagttgcgaatgtcgttgtaccgactgctgtttattttagcgaaaaatataacgatgttgtacgtggcactaccgagcaaggctaccgtgttagctcgtaccttcctctgttacctacggaaaaaatcacgaaggtattcggagacgaggcatca BBa_K2027054_sequence 1 atggaattgtacaacggggaacgcccctcggtttttcgtttgttaggaaagtacatgcgtaaagggttatactctattttaacacagggaccaattccaacgcacatcgcctttatcctggacggaaatcgtcgttttgcaaaaaaacacaagttgcccgaaggcggtggccacaaggcgggttttcttgcccttctgaatgtcctgacatattgttatgagctgggggtaaagtatgcaactatttacgccttttccattgacaactttcgccgtaaacctcacgaagtccaatacgtcatggatctgatgttggaaaagatcgaaggcatgattatggaagagtcgatcatcaacgcttatgacatttgtgtccgctttgtcggtaatttaaaattgttatccgagccggttaagacagccgccgacaaaatcatgcgcgctacagccaacaattcaaaatgtgtccttttaatcgcggtatgttacacgtccacagacgaaattgtacatgccgtagaagaaagttcagaacttaattcgaacgaggtatgcaacaatcaagagttagaggaggcaaatgcgactgggtcgggcaccgtgattcaaatcgaaaacatggaatcttatagcggaattaaacttgtcgatttggagaaaaacacctatatcaacccttaccctgatgtacttattcgtacatcaggcgagacacgtttatcaaattacttgctgtggcagacgactaattgcattttatactccccgcacgctctttggccggagattgggctgcgtcacgtcgtctgggctgtgattaattttcagcgccactactcctacttggagaaacataaggagtatcttaaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2027052_sequence 1 gactacaaagacgatgacgacaagtgttgtcctggctgttgcggtgctggtggccatcatcaccatcaccat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z