LOCUS       BBa_K204096             2502 bp    DNA     linear       17-May-2024
DEFINITION  ptet+crtE+crtB+crtZ
ACCESSION   BBa_K204096
VERSION     BBa_K204096.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..66
                     /label=BBa_K118012
     CDS             73..984
                     /label=BBa_I742151
     misc_feature    993..995
                     /label=SacI
     RBS             996..1002
                     /label=rbs
     RBS             993..1002
                     /label=BBa_J15001
     CDS             1009..1938
                     /label=crtB coding sequence
     CDS             1009..1941
                     /label=BBa_K118002
     RBS             1950..1965
                     /label=BBa_I742156
     CDS             1972..2499
                     /label=crtZ
     CDS             1972..2502
                     /label=BBa_I742157
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 aggaggtact agatgacggt ctgcgcaaaa aaacacgttc atctcactcg cgatgctgcg
      121 gagcagttac tggctgatat tgatcgacgc cttgatcagt tattgcccgt ggagggagaa
      181 cgggatgttg tgggtgccgc gatgcgtgaa ggtgcgctgg caccgggaaa acgtattcgc
      241 cccatgttgc tgttgctgac cgcccgcgat ctgggttgcg ctgtcagcca tgacggatta
      301 ctggatttgg cctgtgcggt ggaaatggtc cacgcggctt cgctgatcct tgacgatatg
      361 ccctgcatgg acgatgcgaa gctgcggcgc ggacgcccta ccattcattc tcattacgga
      421 gagcatgtgg caatactggc ggcggttgcc ttgctgagta aagcctttgg cgtaattgcc
      481 gatgcagatg gcctcacgcc gctggcaaaa aatcgggcgg tttctgaact gtcaaacgcc
      541 atcggcatgc aaggattggt tcagggtcag ttcaaggatc tgtctgaagg ggataagccg
      601 cgcagcgctg aagctatttt gatgacgaat cactttaaaa ccagcacgct gttttgtgcc
      661 tccatgcaga tggcctcgat tgttgcgaat gcctccagcg aagcgcgtga ttgcctgcat
      721 cgtttttcac ttgatcttgg tcaggcattt caactgctgg acgatttgac cgatggcatg
      781 accgacaccg gtaaggatag caatcaggac gccggtaaat cgacgctggt caatctgtta
      841 ggcccgaggg cggttgaaga acgtctgaga caacatcttc agcttgccag tgagcatctc
      901 tctgcggcct gccaacacgg gcacgccact caacatttta ttcaggcctg gtttgacaaa
      961 aaactcgctg ccgtcagtta ataatactag agctcaagga ggtactagat gaataatccg
     1021 tcgttactca atcatgcggt cgaaacgatg gcagttggct cgaaaagttt tgcgacagcc
     1081 tcaaagttat ttgatgcaaa aacccggcgc agcgtactga tgctctacgc ctggtgccgc
     1141 cattgtgacg atgttattga cgatcagacg ctgggctttc aggcccggca gcctgcctta
     1201 caaacgcccg aacaacgtct gatgcaactt gagatgaaaa cgcgccaggc ctatgcagga
     1261 tcgcagatgc acgaaccggc gtttgcggct tttcaggaag tggctatggc tcatgatatc
     1321 gccccggctt acgcgtttga tcatctggaa ggcttcgcca tggatgtacg cgaagcgcaa
     1381 tacagccaac tggatgatac gctgcgctat tgctatcacg ttgcaggcgt tgtcggcttg
     1441 atgatggcgc aaatcatggg cgtgcgggat aacgccacgc tggaccgcgc ctgtgacctt
     1501 gggctggcat ttcagttgac caatattgct cgcgatattg tggacgatgc gcatgcgggc
     1561 cgctgttatc tgccggcaag ctggctggag catgaaggtc tgaacaaaga gaattatgcg
     1621 gcacctgaaa accgtcaggc gctgagccgt atcgcccgtc gtttggtgca ggaagcagaa
     1681 ccttactatt tgtctgccac agccggcctg gcagggttgc ccctgcgttc cgcctgggca
     1741 atcgctacgg cgaagcaggt ttaccggaaa ataggtgtca aagttgaaca ggccggtcag
     1801 caagcctggg atcagcggca gtcaacgacc acgcccgaaa aattaacgct gctgctggcc
     1861 gcctctggtc aggcccttac ttcccggatg cgggctcatc ctccccgccc tgcgcatctc
     1921 tggcagcgcc cgctctaata atactagagc tctaccggag aaatttacta gatgttgtgg
     1981 atttggaatg ccctgatcgt tttcgttacc gtgattggca tggaagtgat tgctgcactg
     2041 gcacacaaat acatcatgca cggctggggt tggggatggc atctttcaca tcatgaaccg
     2101 cgtaaaggtg cgtttgaagt taacgatctt tatgccgtgg tttttgctgc attatcgatc
     2161 ctgctgattt atctgggcag tacaggaatg tggccgctcc agtggattgg cgcaggtatg
     2221 acggcgtatg gattactcta ttttatggtg cacgacgggc tggtgcatca acgttggcca
     2281 ttccgctata ttccacgcaa gggctacctc aaacggttgt atatggcgca ccgtatgcat
     2341 cacgccgtca ggggcaaaga aggttgtgtt tcttttggct tcctctatgc gccgcccctg
     2401 tcaaaacttc aggcgacgct ccgggaaaga catggcgcta gagcgggcgc tgccagagat
     2461 gcgcagggcg gggaggatga gcccgcatcc gggaagtaat aa
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