LOCUS       BBa_K2041020             986 bp    DNA     linear       15-May-2024
DEFINITION  promoter-RBS-binding site of miR-155-tetR-T
ACCESSION   BBa_K2041020
VERSION     BBa_K2041020.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23104
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    64..86
                     /label=the binding site 1 of miR-155
     misc_feature    88..110
                     /label=the binding site 2 of miR-155
     misc_feature    136..158
                     /label=the binding site 4 of miR-155
     misc_feature    112..134
                     /label=the binding site 3 of miR-155
     misc_feature    64..158
                     /label=BBa_K2041018
     CDS             168..784
                     /label=tetR
     misc_feature    785..818
                     /label=SsrA
     misc_feature    825..849
                     /label=Help:Barcodes
     CDS             165..849
                     /label=BBa_C0040
     stem_loop       869..912
                     /label=stem_loop
     misc_feature    858..937
                     /label=BBa_B0010
     terminator      858..937
                     /label=BBa_B0010
     stem_loop       953..972
                     /label=T7 TE
     misc_feature    979..979
                     /label=stop
     polyA_site      973..986
                     /label=polya
     misc_feature    946..986
                     /label=BBa_B0012
     terminator      946..986
                     /label=BBa_B0012
     terminator      858..986
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta
       61 gagaccccta tcacgattag cattaagacc cctatcacga ttagcattaa gacccctatc
      121 acgattagca ttaagacccc tatcacgatt agcattaata ctagatgtcc agattagata
      181 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa
      241 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa
      301 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc
      361 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt
      421 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta
      481 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt
      541 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat
      601 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta
      661 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct
      721 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg
      781 ggtccgctgc aaacgacgaa aactacgctt tagtagctta ataacactga tagtgctagt
      841 gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      901 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
      961 ttcgggtggg cctttctgcg tttata
//