BBa_K1583054 1 BBa_K1583054 Non-coding scar site in between RBS and gene 2015-09-03T11:00:00Z 2015-09-18T08:03:55Z The RBS and CsgC were synthesized from the genomic sequence of E.coli K-12 MG1655. The promoter is part of the constitutive promoter family made by John Anderson from iGEM Berkeley 2006. CsgC is positively involved in the extracellular aggregation of CsgA into amyloid nanowires (curli assembly) in E.coli functioning as a chaperone for CsgA. false false _2000_ 24478 24463 9 false We inserted non-coding scar sequences after the promoter and RBS to prevent missing initial codons during transcription/translation. false Stefan Robert Marsden annotation2475152 1 Scar range2475152 1 1 6 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_J34801 1 BBa_J34801 ribosome binding site 2006-10-25T11:00:00Z 2015-08-31T04:08:46Z a Released HQ 2013 a false false _62_ 0 1085 62 In stock false a false Alexandra Choutko BBa_K2042015 1 BBa_K2042015 LDH* Generator 2016-08-29T11:00:00Z 2016-10-10T09:23:20Z synthetic synthesis Pputida promotor+igemrbs+ldh+terminqtor false false _2509_ 31392 31409 9 false plamid pseva224 for increasing lactate false Amir Pandi component2482888 1 BBa_K2042008 component2482895 1 BBa_B0015 component2482883 1 BBa_K2042009 component2482887 1 BBa_K1583054 component2482882 1 BBa_K2042018 component2482885 1 BBa_J34801 component2482884 1 BBa_K2042019 annotation2482895 1 BBa_B0015 range2482895 1 1072 1200 annotation2482884 1 BBa_K2042019 range2482884 1 45 51 annotation2482887 1 BBa_K1583054 range2482887 1 64 69 annotation2482882 1 BBa_K2042018 range2482882 1 1 4 annotation2482888 1 BBa_K2042008 range2482888 1 70 1071 annotation2482885 1 BBa_J34801 range2482885 1 52 63 annotation2482883 1 BBa_K2042009 range2482883 1 5 44 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_K2042018 1 BBa_K2042018 Scar before LDH*constitutive promoter 2016-09-10T11:00:00Z 2016-10-10T09:40:44Z None Random nucleotide before LDH promotor false false _2509_ 31392 31392 9 false None false Toky RATOVOMANANA BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K2042019 1 BBa_K2042019 Spacer in between promoter and coding sequence 2016-09-10T11:00:00Z 2016-10-10T09:56:27Z None between LDH prom and RBS false false _2509_ 31392 31392 9 false None false Toky RATOVOMANANA annotation2495251 1 Spacer range2495251 1 1 7 BBa_K2042008 1 D-LDH* Engineered D-Lactate dehydrogenase (D-LDH*) 2016-08-29T11:00:00Z 2016-10-08T11:58:59Z Synthetic synthesis This is the coding sequence of lactate deshydrogenase that can super efficiently use NADPH and NADH as cofactor false false _2509_ 31392 31409 9 false This enwy;e is for increasing lactate false Amir Pandi annotation2512511 1 Start range2512511 1 1 3 annotation2512510 1 D-LDH* range2512510 1 1 1002 annotation2512513 1 Stop range2512513 1 1000 1002 BBa_K2042009 1 BBa_K2042009 P. putida constitutive promoter 2016-08-29T11:00:00Z 2016-10-10T09:20:26Z synthetic synthesis It is the coding of pseudomonas putida constitutive promotor false false _2509_ 31392 31409 9 false This promotor will constitutively express LDH false Amir Pandi annotation2488299 1 pTN8-P1 range2488299 1 1 40 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K2042009_sequence 1 cgactccacttgaacaatgttgtggtaccatttaaaacta BBa_K2042019_sequence 1 ggatccg BBa_K2042018_sequence 1 tagc BBa_K2042015_sequence 1 tagccgactccacttgaacaatgttgtggtaccatttaaaactaggatccgaaagaggagaaatactagatgaccaaaatcttcgcgtatgcgatccgtgaagatgagaaaccctttctcaaggagtgggaggatgcccataaagatgtcgaggtcgagtacacggataagctcctcacgcctgaaaccgtggcattggccaagggggccgacggtgtggtcgtctatcagcagctcgattacacggcggaaaccttgcaagctttggcggacaacggtatcaccaagatgtcgttgcgtaacgtgggggtcgataatattgacatggcgaaggcaaaagaattggggtttcaaattaccaatgtgccagtgtactccccgaacgcaatcgcagaacatgcggctattcaagcagctcgtatcttgcgccaggacaaggcgatggatgagaaagtggcacgccatgatctccgctgggctccaaccattggccgggaggtccgtgaccaagtggtcggggtgatcggcacgggtcacattggtcaagtcttcatgcagatcatggaagggtttggggccaaggtcattgcatacagccgcacgcggaacccagagctggagaagaaggggtattacgtggatagcctcgatgacttgtataaacaggccgacgtgatctccctccacgtgcccgatgtgccggccaatgtgcacatgatcaatgacgaatccatcgcaaaaatgaaacaagacgtggtcattgtgaatgtcagccgtgggcccctcgtcgataccgacgccgtcattcgcgggttggacagcgggaaaatcttcggctatgcaatggatgtctacgagggcgaggtcggtatcttcaatgaggactgggagggtaaagaatttcctgatgcacgcttggcggacctgattgcccgtcctaatgtgctggtcacccctcacacggcattttacaccacgcacgcagtgcgtaatatggtcgtgaaggctttcgacaacaacctcgaattggtggaggggaaggaagccgaaaccccagtcaaggtggggtgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J34801_sequence 1 aaagaggagaaa BBa_K1583054_sequence 1 tactag BBa_K2042008_sequence 1 atgaccaaaatcttcgcgtatgcgatccgtgaagatgagaaaccctttctcaaggagtgggaggatgcccataaagatgtcgaggtcgagtacacggataagctcctcacgcctgaaaccgtggcattggccaagggggccgacggtgtggtcgtctatcagcagctcgattacacggcggaaaccttgcaagctttggcggacaacggtatcaccaagatgtcgttgcgtaacgtgggggtcgataatattgacatggcgaaggcaaaagaattggggtttcaaattaccaatgtgccagtgtactccccgaacgcaatcgcagaacatgcggctattcaagcagctcgtatcttgcgccaggacaaggcgatggatgagaaagtggcacgccatgatctccgctgggctccaaccattggccgggaggtccgtgaccaagtggtcggggtgatcggcacgggtcacattggtcaagtcttcatgcagatcatggaagggtttggggccaaggtcattgcatacagccgcacgcggaacccagagctggagaagaaggggtattacgtggatagcctcgatgacttgtataaacaggccgacgtgatctccctccacgtgcccgatgtgccggccaatgtgcacatgatcaatgacgaatccatcgcaaaaatgaaacaagacgtggtcattgtgaatgtcagccgtgggcccctcgtcgataccgacgccgtcattcgcgggttggacagcgggaaaatcttcggctatgcaatggatgtctacgagggcgaggtcggtatcttcaatgaggactgggagggtaaagaatttcctgatgcacgcttggcggacctgattgcccgtcctaatgtgctggtcacccctcacacggcattttacaccacgcacgcagtgcgtaatatggtcgtgaaggctttcgacaacaacctcgaattggtggaggggaaggaagccgaaaccccagtcaaggtggggtga BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z