LOCUS       BBa_K2055000            1374 bp    DNA     linear       18-May-2024
DEFINITION  Gold/copper inducible regulator
ACCESSION   BBa_K2055000
VERSION     BBa_K2055000.1  
FEATURES             Location/Qualifiers
     promoter        1..39
                     /label=pGolS
     promoter        1..39
                     /label=BBa_K2055169
     misc_feature    52..55
                     /label=conserved
     RBS             48..59
                     /label=BBa_B0034
     CDS             66..482
                     /label=BBa_K2055069
     misc_feature    495..498
                     /label=conserved
     RBS             491..502
                     /label=BBa_B0034
     RBS             511..525
                     /label=RBS-1\Strong
     RBS             518..522
                     /label=RBS
     misc_feature    511..525
                     /label=BBa_B0030
     RBS             511..525
                     /label=BBa_B0030
     CDS             532..1206
                     /label=mrfp1
     misc_feature    1213..1237
                     /label=Help:Barcodes
     CDS             532..1237
                     /label=BBa_E1010
     stem_loop       1257..1300
                     /label=stem_loop
     misc_feature    1246..1325
                     /label=BBa_B0010
     terminator      1246..1325
                     /label=BBa_B0010
     stem_loop       1341..1360
                     /label=T7 TE
     misc_feature    1367..1367
                     /label=stop
     polyA_site      1361..1374
                     /label=polya
     misc_feature    1334..1374
                     /label=BBa_B0012
     terminator      1334..1374
                     /label=BBa_B0012
     terminator      1246..1374
                     /label=BBa_B0015
     misc_feature    511..1374
                     /label=BBa_K516030
ORIGIN
        1 cttgaccttc ccacaatggc aagctttagg ctttctgatt actagagaaa gaggagaaat
       61 actagatgaa tattagcgat gtggcgaaaa aaaccggttt aaccagcaaa gccattcggt
      121 tttatgaaga gaaagggctg gtgacgccgc cattacgtag cgaaaatggt tatcgcacct
      181 acacgcagaa gcatttaaac gagctgacgt tgctgcgaca ggcgaggcag gtggggttta
      241 atctggaaga gtgtggcgaa ctggtcaatc tgtttaacga tccgcgacgc catagcgcag
      301 atgtgaaaaa gcggacgctg gaaaaggtcg cggagatcga gcgccatatc agcgagctgc
      361 aatcaatgcg cgatcaactg ttggcgctgg cggagagttg tcccggcgat gacagcgccg
      421 actgcccgat tattgataat ctttccggct gctgtcatca caaggcgcaa aagccacgtt
      481 gatactagag aaagaggaga aatactagag attaaagagg agaaatacta gatggcttcc
      541 tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac
      601 ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc
      661 gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg
      721 cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg
      781 aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt
      841 gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa
      901 ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg
      961 gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg
     1021 cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct
     1081 aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc
     1141 cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc
     1201 ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa
     1261 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1321 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//