BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation7025 1 BBa_B0030 range7025 1 1 15 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K2055000 1 BBa_K2055000 Gold/copper inducible regulator 2016-09-28T11:00:00Z 2016-10-19T08:24:26Z Its organism of origin is Salmonella typhimurium. DNA construct constituted by the pgolS promoter and the coding sequence of CueR. As the pgolS promoter has a basal transcription level, some CueR would be constantly expressed. CueR binds either copper or gold, though it is more selective for copper. CueR is a transcription factor that stimulates the transcription at the pgolS promoter when bound to copper/gold. Thus, this construct increases its expression in the presence of gold or copper through the production of a copper/gold sensitive protein. false false _2523_ 30725 30723 9 false The coding sequence is optimized for its expression in C. violaceum false Yili Natalia Padilla Flores, Jos?? Garc??a Lima, Marcelo ??lvarez Mata component2523933 1 BBa_B0034 component2523947 1 BBa_K516030 component2523928 1 BBa_K2055169 component2523930 1 BBa_B0034 component2523931 1 BBa_K2055069 annotation2523930 1 BBa_B0034 range2523930 1 48 59 annotation2523928 1 BBa_K2055169 range2523928 1 1 39 annotation2523933 1 BBa_B0034 range2523933 1 491 502 annotation2523947 1 BBa_K516030 range2523947 1 511 1374 annotation2523931 1 BBa_K2055069 range2523931 1 66 482 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K516030 1 BBa_K516030 mRFP protein generator TERM+ RBS+(B0030) 2011-09-07T11:00:00Z 2015-05-08T01:12:32Z Registry Released HQ 2013 mRFP protein generator TERM+ RBS+(B0030) false false _681_ 0 4150 9 In stock true -- false Nicolo' Politi, Federica Sampietro, Davide Bianchini component2250572 1 BBa_B0030 component2250576 1 BBa_E1010 component2250583 1 BBa_B0015 annotation2250583 1 BBa_B0015 range2250583 1 736 864 annotation2250572 1 BBa_B0030 range2250572 1 1 15 annotation2250576 1 BBa_E1010 range2250576 1 22 727 BBa_K2055069 1 CueR cueR DNA-binding transcriptional regulator 2016-09-28T11:00:00Z 2016-09-29T04:34:44Z Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S CueR binds either copper or gold, though it is more selective for copper. CueR is a transcription factor that stimulates the transcription at the pgolS promoter when bound to copper/gold. false false _2523_ 30723 30723 9 false The coding sequence is optimized for its expression in C. violaceum. false Yili Natalia Padilla Flores BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation1014044 1 mrfp1 range1014044 1 1 675 annotation2214014 1 Help:Barcodes range2214014 1 682 706 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_K2055169 1 BBa_K2055169 Gold inducible promoter 2016-09-28T11:00:00Z 2016-09-29T05:35:57Z Salmonella typhimurium LT2 It is positively regulated by the transcription factor GolS. GolS induces transcription from this promoter once GolS is activated upon binding a gold ion. false false _2523_ 30723 30723 9 false This part was de novo synthesized by iGEM10_UIUC-Illinois. http://parts.igem.org/Part:BBa_K1127008 false Yili Natalia Padilla Flores annotation2485449 1 pGolS range2485449 1 1 39 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_B0030_sequence 1 attaaagaggagaaa BBa_K516030_sequence 1 attaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2055000_sequence 1 cttgaccttcccacaatggcaagctttaggctttctgattactagagaaagaggagaaatactagatgaatattagcgatgtggcgaaaaaaaccggtttaaccagcaaagccattcggttttatgaagagaaagggctggtgacgccgccattacgtagcgaaaatggttatcgcacctacacgcagaagcatttaaacgagctgacgttgctgcgacaggcgaggcaggtggggtttaatctggaagagtgtggcgaactggtcaatctgtttaacgatccgcgacgccatagcgcagatgtgaaaaagcggacgctggaaaaggtcgcggagatcgagcgccatatcagcgagctgcaatcaatgcgcgatcaactgttggcgctggcggagagttgtcccggcgatgacagcgccgactgcccgattattgataatctttccggctgctgtcatcacaaggcgcaaaagccacgttgatactagagaaagaggagaaatactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2055069_sequence 1 atgaatattagcgatgtggcgaaaaaaaccggtttaaccagcaaagccattcggttttatgaagagaaagggctggtgacgccgccattacgtagcgaaaatggttatcgcacctacacgcagaagcatttaaacgagctgacgttgctgcgacaggcgaggcaggtggggtttaatctggaagagtgtggcgaactggtcaatctgtttaacgatccgcgacgccatagcgcagatgtgaaaaagcggacgctggaaaaggtcgcggagatcgagcgccatatcagcgagctgcaatcaatgcgcgatcaactgttggcgctggcggagagttgtcccggcgatgacagcgccgactgcccgattattgataatctttccggctgctgtcatcacaaggcgcaaaagccacgttga BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2055169_sequence 1 cttgaccttcccacaatggcaagctttaggctttctgat BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z