LOCUS       BBa_K206003              999 bp    DNA     linear       21-May-2024
DEFINITION  RFP generator for testing pBAD weak
ACCESSION   BBa_K206003
VERSION     BBa_K206003.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206001
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     CDS             157..831
                     /label=mrfp1
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             157..837
                     /label=BBa_E1010
     stem_loop       882..925
                     /label=stem_loop
     misc_feature    871..950
                     /label=BBa_B0010
     terminator      871..950
                     /label=BBa_B0010
     stem_loop       966..985
                     /label=T7 TE
     misc_feature    992..992
                     /label=stop
     polyA_site      986..999
                     /label=polya
     misc_feature    959..999
                     /label=BBa_B0012
     terminator      959..999
                     /label=BBa_B0012
     misc_feature    139..999
                     /label=BBa_I13507
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agctttttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc
      181 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa
      241 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt
      301 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt
      361 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg
      421 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc
      481 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac
      541 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa
      601 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa
      661 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag
      721 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac
      781 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc
      841 tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt
      901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      961 acactggctc accttcgggt gggcctttct gcgtttata
//