LOCUS       BBa_K2066024            1083 bp    DNA     linear       19-May-2024
DEFINITION  pTac+RiboJ+sfGFP
ACCESSION   BBa_K2066024
VERSION     BBa_K2066024.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     promoter        41..80
                     /label=Ptac
     promoter        47..52
                     /label=-35
     promoter        69..73
                     /label=-10
     misc_feature    76..101
                     /label=lacO1 site
     promoter        41..101
                     /label=BBa_K864400
     misc_feature    102..182
                     /label=BBa_K2066506
     RBS             183..194
                     /label=BBa_K2066510
     misc_feature    195..914
                     /label=BBa_K2066509
     stem_loop       926..969
                     /label=stem_loop
     misc_feature    915..994
                     /label=BBa_B0010
     terminator      915..994
                     /label=BBa_B0010
     stem_loop       1010..1029
                     /label=T7 TE
     misc_feature    1036..1036
                     /label=stop
     polyA_site      1030..1043
                     /label=polya
     misc_feature    1003..1043
                     /label=BBa_B0012
     terminator      1003..1043
                     /label=BBa_B0012
     terminator      915..1043
                     /label=BBa_B0015
     misc_feature    1044..1083
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc gagctgttga caattaatca
       61 tcggctcgta taatgtgtgg aattgtgagc ggataacaat tagctgtcac cggatgtgct
      121 ttccggtctg atgagtccgt gaggacgaaa cagcctctac aaataatttt gtttaaacta
      181 gaaggaggaa aaaaatgcgt aaaggcgaag agctgttcac tggtgtcgtc cctattctgg
      241 tggaactgga tggtgatgtc aacggtcata agttttccgt gcgtggcgag ggtgaaggtg
      301 acgcaactaa tggtaaactg acgctgaagt tcatctgtac tactggtaaa ctgccggtac
      361 cttggccgac tctggtaacg acgctgactt atggtgttca gtgctttgct cgttatccgg
      421 accatatgaa gcagcatgac ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac
      481 gcacgatttc ctttaaggat gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag
      541 gcgataccct ggtaaaccgc attgagctga aaggcattga ctttaaagaa gacggcaata
      601 tcctgggcca taagctggaa tacaatttta acagccacaa tgtgtacatt accgcagata
      661 aacaaaaaaa tggcattaaa gcgaatttca aaattcgcca caacgtggag gatggcagcg
      721 tgcagctggc tgatcactac cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc
      781 cagacaatca ctatctgagc acgcaaagcg ttctgtctaa agatccgaac gagaaacgcg
      841 atcatatggt tctgctggag ttcgtaaccg cagcgggcat cacgcatggt atggatgaac
      901 tgtacaaatg atgaccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      961 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1021 gggtgggcct ttctgcgttt atagcactga aggtcctcaa tcgcactgga aacatcaagg
     1081 tcg
//