LOCUS       BBa_K2066025            1803 bp    DNA     linear       17-May-2024
DEFINITION  pTac+RiboJ+cIGFP
ACCESSION   BBa_K2066025
VERSION     BBa_K2066025.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     promoter        41..80
                     /label=Ptac
     promoter        47..52
                     /label=-35
     promoter        69..73
                     /label=-10
     misc_feature    76..101
                     /label=lacO1 site
     promoter        41..101
                     /label=BBa_K864400
     misc_feature    102..182
                     /label=BBa_K2066506
     RBS             183..194
                     /label=BBa_K2066515
     misc_feature    195..1634
                     /label=BBa_K2066511
     stem_loop       1646..1689
                     /label=stem_loop
     misc_feature    1635..1714
                     /label=BBa_B0010
     terminator      1635..1714
                     /label=BBa_B0010
     stem_loop       1730..1749
                     /label=T7 TE
     misc_feature    1756..1756
                     /label=stop
     polyA_site      1750..1763
                     /label=polya
     misc_feature    1723..1763
                     /label=BBa_B0012
     terminator      1723..1763
                     /label=BBa_B0012
     terminator      1635..1763
                     /label=BBa_B0015
     misc_feature    1764..1803
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc gagctgttga caattaatca
       61 tcggctcgta taatgtgtgg aattgtgagc ggataacaat tagctgtcac cggatgtgct
      121 ttccggtctg atgagtccgt gaggacgaaa cagcctctac aaataatttt gtttaaacta
      181 gaaccatgaa aaaagtgagc acaaaaaaga aaccattaac acaagagcag cttgaggacg
      241 cacgtcgcct taaagcaatt tatgaaaaaa agaaaaatga acttggctta tcccaggaat
      301 ctgtcgcaga caagatgggg atggggcagt caggcgttgg tgctttattt aatggcatca
      361 atgcattaaa tgcttataac gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag
      421 aatttagccc ttcaatcgcc agagaaatct acgagatgta tgaagcggtt agtatgcagc
      481 cgtcacttag aagtgagtat gagtaccctg ttttttctca tgttcaggca gggatgttct
      541 cacctgagct tagaaccttt accaaaggtg atgcggagag atgggtaagc acaaccaaaa
      601 aagccagtga ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag
      661 gctccaagcc aagctttcct gacggaatgt taattctcgt tgaccctgag caggctgttg
      721 agccaggtga tttctgcata gccagacttg ggggtgatga gtttaccttc aagaaactga
      781 tcagggatag cggtcaggtg tttttacaac cactaaaccc acagtaccca atgatcccat
      841 gcaatgagag ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt
      901 ttggcggcgg tggcggtcgt aaaggcgaag agctgttcac tggtgtcgtc cctattctgg
      961 tggaactgga tggtgatgtc aacggtcata agttttccgt gcgtggcgag ggtgaaggtg
     1021 acgcaactaa tggtaaactg acgctgaagt tcatctgtac tactggtaaa ctgccggtac
     1081 cttggccgac tctggtaacg acgctgactt atggtgttca gtgctttgct cgttatccgg
     1141 accatatgaa gcagcatgac ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac
     1201 gcacgatttc ctttaaggat gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag
     1261 gcgataccct ggtaaaccgc attgagctga aaggcattga ctttaaagaa gacggcaata
     1321 tcctgggcca taagctggaa tacaatttta acagccacaa tgtgtacatt accgcagata
     1381 aacaaaaaaa tggcattaaa gcgaatttca aaattcgcca caacgtggag gatggcagcg
     1441 tgcagctggc tgatcactac cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc
     1501 cagacaatca ctatctgagc acgcaaagcg ttctgtctaa agatccgaac gagaaacgcg
     1561 atcatatggt tctgctggag ttcgtaaccg cagcgggcat cacgcatggt atggatgaac
     1621 tgtacaaatg atgaccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1681 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1741 gggtgggcct ttctgcgttt atagcactga aggtcctcaa tcgcactgga aacatcaagg
     1801 tcg
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