LOCUS       BBa_K2066030            1008 bp    DNA     linear       17-May-2024
DEFINITION  pTAC sfGFP
ACCESSION   BBa_K2066030
VERSION     BBa_K2066030.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     promoter        41..80
                     /label=Ptac
     promoter        47..52
                     /label=-35
     promoter        69..73
                     /label=-10
     misc_feature    76..101
                     /label=lacO1 site
     promoter        41..101
                     /label=BBa_K864400
     misc_feature    102..107
                     /label=BBa_K2066528
     RBS             108..119
                     /label=BBa_K2066510
     misc_feature    120..839
                     /label=BBa_K2066509
     stem_loop       851..894
                     /label=stem_loop
     misc_feature    840..919
                     /label=BBa_B0010
     terminator      840..919
                     /label=BBa_B0010
     stem_loop       935..954
                     /label=T7 TE
     misc_feature    961..961
                     /label=stop
     polyA_site      955..968
                     /label=polya
     misc_feature    928..968
                     /label=BBa_B0012
     terminator      928..968
                     /label=BBa_B0012
     terminator      840..968
                     /label=BBa_B0015
     misc_feature    969..1008
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc gagctgttga caattaatca
       61 tcggctcgta taatgtgtgg aattgtgagc ggataacaat tactagaagg aggaaaaaaa
      121 tgcgtaaagg cgaagagctg ttcactggtg tcgtccctat tctggtggaa ctggatggtg
      181 atgtcaacgg tcataagttt tccgtgcgtg gcgagggtga aggtgacgca actaatggta
      241 aactgacgct gaagttcatc tgtactactg gtaaactgcc ggtaccttgg ccgactctgg
      301 taacgacgct gacttatggt gttcagtgct ttgctcgtta tccggaccat atgaagcagc
      361 atgacttctt caagtccgcc atgccggaag gctatgtgca ggaacgcacg atttccttta
      421 aggatgacgg cacgtacaaa acgcgtgcgg aagtgaaatt tgaaggcgat accctggtaa
      481 accgcattga gctgaaaggc attgacttta aagaagacgg caatatcctg ggccataagc
      541 tggaatacaa ttttaacagc cacaatgtgt acattaccgc agataaacaa aaaaatggca
      601 ttaaagcgaa tttcaaaatt cgccacaacg tggaggatgg cagcgtgcag ctggctgatc
      661 actaccagca aaacactcca atcggtgatg gtcctgttct gctgccagac aatcactatc
      721 tgagcacgca aagcgttctg tctaaagatc cgaacgagaa acgcgatcat atggttctgc
      781 tggagttcgt aaccgcagcg ggcatcacgc atggtatgga tgaactgtac aaatgatgac
      841 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
      901 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
      961 cgtttatagc actgaaggtc ctcaatcgca ctggaaacat caaggtcg
//