LOCUS       BBa_K2066031            1728 bp    DNA     linear       17-May-2024
DEFINITION  pTAC cI-GFP
ACCESSION   BBa_K2066031
VERSION     BBa_K2066031.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     promoter        41..80
                     /label=Ptac
     promoter        47..52
                     /label=-35
     promoter        69..73
                     /label=-10
     misc_feature    76..101
                     /label=lacO1 site
     promoter        41..101
                     /label=BBa_K864400
     misc_feature    102..107
                     /label=BBa_K2066528
     RBS             108..119
                     /label=BBa_K2066515
     misc_feature    120..1559
                     /label=BBa_K2066511
     stem_loop       1571..1614
                     /label=stem_loop
     misc_feature    1560..1639
                     /label=BBa_B0010
     terminator      1560..1639
                     /label=BBa_B0010
     stem_loop       1655..1674
                     /label=T7 TE
     misc_feature    1681..1681
                     /label=stop
     polyA_site      1675..1688
                     /label=polya
     misc_feature    1648..1688
                     /label=BBa_B0012
     terminator      1648..1688
                     /label=BBa_B0012
     terminator      1560..1688
                     /label=BBa_B0015
     misc_feature    1689..1728
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc gagctgttga caattaatca
       61 tcggctcgta taatgtgtgg aattgtgagc ggataacaat tactagaacc atgaaaaaag
      121 tgagcacaaa aaagaaacca ttaacacaag agcagcttga ggacgcacgt cgccttaaag
      181 caatttatga aaaaaagaaa aatgaacttg gcttatccca ggaatctgtc gcagacaaga
      241 tggggatggg gcagtcaggc gttggtgctt tatttaatgg catcaatgca ttaaatgctt
      301 ataacgccgc attgcttgca aaaattctca aagttagcgt tgaagaattt agcccttcaa
      361 tcgccagaga aatctacgag atgtatgaag cggttagtat gcagccgtca cttagaagtg
      421 agtatgagta ccctgttttt tctcatgttc aggcagggat gttctcacct gagcttagaa
      481 cctttaccaa aggtgatgcg gagagatggg taagcacaac caaaaaagcc agtgattctg
      541 cattctggct tgaggttgaa ggtaattcca tgaccgcacc aacaggctcc aagccaagct
      601 ttcctgacgg aatgttaatt ctcgttgacc ctgagcaggc tgttgagcca ggtgatttct
      661 gcatagccag acttgggggt gatgagttta ccttcaagaa actgatcagg gatagcggtc
      721 aggtgttttt acaaccacta aacccacagt acccaatgat cccatgcaat gagagttgtt
      781 ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga gacgtttggc ggcggtggcg
      841 gtcgtaaagg cgaagagctg ttcactggtg tcgtccctat tctggtggaa ctggatggtg
      901 atgtcaacgg tcataagttt tccgtgcgtg gcgagggtga aggtgacgca actaatggta
      961 aactgacgct gaagttcatc tgtactactg gtaaactgcc ggtaccttgg ccgactctgg
     1021 taacgacgct gacttatggt gttcagtgct ttgctcgtta tccggaccat atgaagcagc
     1081 atgacttctt caagtccgcc atgccggaag gctatgtgca ggaacgcacg atttccttta
     1141 aggatgacgg cacgtacaaa acgcgtgcgg aagtgaaatt tgaaggcgat accctggtaa
     1201 accgcattga gctgaaaggc attgacttta aagaagacgg caatatcctg ggccataagc
     1261 tggaatacaa ttttaacagc cacaatgtgt acattaccgc agataaacaa aaaaatggca
     1321 ttaaagcgaa tttcaaaatt cgccacaacg tggaggatgg cagcgtgcag ctggctgatc
     1381 actaccagca aaacactcca atcggtgatg gtcctgttct gctgccagac aatcactatc
     1441 tgagcacgca aagcgttctg tctaaagatc cgaacgagaa acgcgatcat atggttctgc
     1501 tggagttcgt aaccgcagcg ggcatcacgc atggtatgga tgaactgtac aaatgatgac
     1561 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1621 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1681 cgtttatagc actgaaggtc ctcaatcgca ctggaaacat caaggtcg
//