LOCUS       BBa_K2066039            2809 bp    DNA     linear       21-May-2024
DEFINITION  Sigma54 Enhancer 57S with DT, UNS Standard
ACCESSION   BBa_K2066039
VERSION     BBa_K2066039.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     misc_feature    41..87
                     /label=BBa_K2066543
     misc_feature    88..236
                     /label=BBa_K2066548
     promoter        237..266
                     /label=BBa_K2066544
     misc_feature    267..288
                     /label=BBa_K2066545
     misc_feature    289..431
                     /label=BBa_K2066547
     CDS             433..1926
                     /label=BBa_K2066546
     misc_feature    1927..2640
                     /label=BBa_K2066518
     stem_loop       2652..2695
                     /label=stem_loop
     misc_feature    2641..2720
                     /label=BBa_B0010
     terminator      2641..2720
                     /label=BBa_B0010
     stem_loop       2736..2755
                     /label=T7 TE
     misc_feature    2762..2762
                     /label=stop
     polyA_site      2756..2769
                     /label=polya
     misc_feature    2729..2769
                     /label=BBa_B0012
     terminator      2729..2769
                     /label=BBa_B0012
     terminator      2641..2769
                     /label=BBa_B0015
     misc_feature    2770..2809
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc tcacaattgc accaacatgg
       61 tgcttaatgt ttccattgaa gcactatatt ggtgcaacat tgtcttcgac gctagctccc
      121 tatcagtgat agagattggg tccggccaga ctcgagcttt gcgccaccgt ctccgcgcgc
      181 tgcgcgcgga tcgcctgcac ggagcgctag cgtccccatg ataacgcctt ttaggggcaa
      241 tttaaaagtt ggcacagatt tcgctttatc ttttttaatt gtgagcgctc acaattaagc
      301 ttggcgaagg tggcaccggg cttggcttat ccatcgctcg taatttgatt gatcagcatt
      361 caggcaaaat tgaatttacc agttggccag ggcataccga gttctcggtt tacctgccta
      421 tcaggaaata aacgtttatg caacgaggga tagtctgggt agtcgatgac gatagttcca
      481 tccgttgggt gcttgaacgt gcgctcgctg gggcaggttt aacctgtacg acgtttgaga
      541 acggcgcaga agtgctggag gcgctggcga gcaaaacgcc ggatgtgctg ctttcagata
      601 tccgtatgcc gggaatggac gggctggcgc tgctcaagca gattaaacag cgccatccaa
      661 tgcttccggt catcattatg accgcacatt ccgatctgga tgctgccgtc agcgcctatc
      721 aacaaggggc gtttgattat ctgcccaaac cgtttgatat cgacgaagca gtggcgctgg
      781 ttgagcgcgc tatcagtcat taccaggaac agcagcagcc gcgtaatgtt cagcttaacg
      841 gcccaacgac cgatatcatc ggcgaagcgc cagccatgca ggacgtgttc cgtattatcg
      901 gtcggctttc gcgttcttct attagcgtgc tgattaacgg cgaatccggc accggtaaag
      961 aactggtcgc tcatgccctg catcgccaca gtccgcgcgc caaagcgccg tttatcgcgc
     1021 tgaatatggc agctatccca aaagatttga tcgaatcaga actgtttggc cacgagaaag
     1081 gcgcgtttac tggcgcgaat accattcgtc aggggcgttt tgaacaggcc gatggcggta
     1141 cattattcct cgacgaaatt ggtgatatgc cgctggatgt gcagacgcgt ttgctgcgcg
     1201 tgctggcaga cggtcagttt taccgcgttg gcggctatgc gccggtgaaa gtggatgtgc
     1261 ggattatcgc tgccactcac cagaatctcg aacagcgagt gcaggaaggt aagttccgtg
     1321 aggatctgtt ccaccgcctg aacgttatcc gcgttcatct gccgccgctg cgcgaacgtc
     1381 gggaagatat tccccgtctg gcgcgccatt ttttacaggt tgccgcgcgc gaactgggcg
     1441 tagaagcgaa gttactgcat ccggaaaccg aagctgctct gacgcgtctg gcgtggccag
     1501 gcaacgtgcg ccagctggaa aacacctgcc gctggctaac ggtgatggcc gccgggcagg
     1561 aagtgttgat tcaggatttg cccggcgaac tgtttgaatc aacggttgcg gagagtactt
     1621 cgcaaatgca accggacagc tgggcgacgc ttcttgcgca gtgggcagac agagcgctgc
     1681 gttccggtca tcaaaatctg ctttccgaag cgcagccaga gctggagcgg acgttactga
     1741 cgaccgcgtt gcgacatacg caggggcata aacaggaagc ggcgcggcta ctcggctggg
     1801 gccgcaacac cctgacgcgt aagttaaaag agctggggat ggagtgattc acagcttgtg
     1861 tgtaaagatt gattattgag cgcaaattgc tggtatttta cgctttactg ttccaggagg
     1921 aaaaaaatgg tgagcaaggg cgaagaagat aacatggcca tcatcaagga gttcatgcgc
     1981 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     2041 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     2101 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     2161 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     2221 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccttg
     2281 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     2341 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     2401 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     2461 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     2521 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     2581 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaataa
     2641 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     2701 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     2761 gcgtttatag cactgaaggt cctcaatcgc actggaaaca tcaaggtcg
//