LOCUS BBa_K2066114 4055 bp DNA linear 13-Jun-2024 DEFINITION Synthetic Enhancer: 3x TetO Binding Cassette (52s) + NRII on UNS ACCESSION BBa_K2066114 VERSION BBa_K2066114.1 FEATURES Location/Qualifiers misc_feature 1..40 /label=BBa_K2066018 misc_feature 41..87 /label=BBa_K2066543 misc_feature 88..236 /label=BBa_K2066531 promoter 237..266 /label=BBa_K2066544 misc_feature 267..288 /label=BBa_K2066545 misc_feature 289..431 /label=BBa_K2066547 CDS 433..1926 /label=BBa_K2066546 misc_feature 1927..2640 /label=BBa_K2066518 stem_loop 2652..2695 /label=stem_loop misc_feature 2641..2720 /label=BBa_B0010 terminator 2641..2720 /label=BBa_B0010 stem_loop 2736..2755 /label=T7 TE misc_feature 2762..2762 /label=stop polyA_site 2756..2769 /label=polya misc_feature 2729..2769 /label=BBa_B0012 terminator 2729..2769 /label=BBa_B0012 terminator 2641..2769 /label=BBa_B0015 misc_feature 2770..2789 /label=BBa_K2066539 misc_feature 2790..2829 /label=BBa_K2066018 misc_feature 2790..2829 /label=BBa_K2066018 promoter 2790..2856 /label=BBa_K2066126 CDS 2857..3906 /label=BBa_K2066538 stem_loop 3918..3961 /label=stem_loop misc_feature 3907..3986 /label=BBa_B0010 terminator 3907..3986 /label=BBa_B0010 stem_loop 4002..4021 /label=T7 TE misc_feature 4028..4028 /label=stop polyA_site 4022..4035 /label=polya misc_feature 3995..4035 /label=BBa_B0012 terminator 3995..4035 /label=BBa_B0012 terminator 3907..4035 /label=BBa_B0015 misc_feature 4036..4055 /label=BBa_K2066540 ORIGIN 1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc tcacaattgc accaacatgg 61 tgcttaatgt ttccattgaa gcactatatt ggtgcaacat tgtcttcgac gctagctctc 121 tatcactgat agggatgtcg gaggctcgcg gactctatca atgatagagt ctcgagtctg 181 gccggatctc tatcactgat agggagctag cgtccccatg ataacgcctt ttaggggcaa 241 tttaaaagtt ggcacagatt tcgctttatc ttttttaatt gtgagcgctc acaattaagc 301 ttggcgaagg tggcaccggg cttggcttat ccatcgctcg taatttgatt gatcagcatt 361 caggcaaaat tgaatttacc agttggccag ggcataccga gttctcggtt tacctgccta 421 tcaggaaata aacgtttatg caacgaggga tagtctgggt agtcgatgac gatagttcca 481 tccgttgggt gcttgaacgt gcgctcgctg gggcaggttt aacctgtacg acgtttgaga 541 acggcgcaga agtgctggag gcgctggcga gcaaaacgcc ggatgtgctg ctttcagata 601 tccgtatgcc gggaatggac gggctggcgc tgctcaagca gattaaacag cgccatccaa 661 tgcttccggt catcattatg accgcacatt ccgatctgga tgctgccgtc agcgcctatc 721 aacaaggggc gtttgattat ctgcccaaac cgtttgatat cgacgaagca gtggcgctgg 781 ttgagcgcgc tatcagtcat taccaggaac agcagcagcc gcgtaatgtt cagcttaacg 841 gcccaacgac cgatatcatc ggcgaagcgc cagccatgca ggacgtgttc cgtattatcg 901 gtcggctttc gcgttcttct attagcgtgc tgattaacgg cgaatccggc accggtaaag 961 aactggtcgc tcatgccctg catcgccaca gtccgcgcgc caaagcgccg tttatcgcgc 1021 tgaatatggc agctatccca aaagatttga tcgaatcaga actgtttggc cacgagaaag 1081 gcgcgtttac tggcgcgaat accattcgtc aggggcgttt tgaacaggcc gatggcggta 1141 cattattcct cgacgaaatt ggtgatatgc cgctggatgt gcagacgcgt ttgctgcgcg 1201 tgctggcaga cggtcagttt taccgcgttg gcggctatgc gccggtgaaa gtggatgtgc 1261 ggattatcgc tgccactcac cagaatctcg aacagcgagt gcaggaaggt aagttccgtg 1321 aggatctgtt ccaccgcctg aacgttatcc gcgttcatct gccgccgctg cgcgaacgtc 1381 gggaagatat tccccgtctg gcgcgccatt ttttacaggt tgccgcgcgc gaactgggcg 1441 tagaagcgaa gttactgcat ccggaaaccg aagctgctct gacgcgtctg gcgtggccag 1501 gcaacgtgcg ccagctggaa aacacctgcc gctggctaac ggtgatggcc gccgggcagg 1561 aagtgttgat tcaggatttg cccggcgaac tgtttgaatc aacggttgcg gagagtactt 1621 cgcaaatgca accggacagc tgggcgacgc ttcttgcgca gtgggcagac agagcgctgc 1681 gttccggtca tcaaaatctg ctttccgaag cgcagccaga gctggagcgg acgttactga 1741 cgaccgcgtt gcgacatacg caggggcata aacaggaagc ggcgcggcta ctcggctggg 1801 gccgcaacac cctgacgcgt aagttaaaag agctggggat ggagtgattc acagcttgtg 1861 tgtaaagatt gattattgag cgcaaattgc tggtatttta cgctttactg ttccaggagg 1921 aaaaaaatgg tgagcaaggg cgaagaagat aacatggcca tcatcaagga gttcatgcgc 1981 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc 2041 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc 2101 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg 2161 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg 2221 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccttg 2281 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc 2341 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag 2401 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac 2461 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac 2521 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag 2581 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaataa 2641 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 2701 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 2761 gcgtttatag cactgaaggt cctcaatcgg gtaggccgga gcaggtgagt cgctctccaa 2821 cgtgaagttt gtcagctatc tgtagcccat ctctgcatgg caacaggcac gcagcccgat 2881 gctgggcaga tcctcaactc gctgattaac agtattttgt taatcgatga caacctggcg 2941 atccattacg ccaaccctgc cgcgcaacaa ctgctcgccc aaagctcccg caaattgttt 3001 ggtacaccgt taccggaact gttgagctac ttctcattaa atatcgagct gatgcaagaa 3061 agtctggagg cggggcaagg ttttaccgat aacgaagtga cgctggtcat cgacgggcgc 3121 tcgcatatcc tttctgtgac ggcccagcgt atgccggacg gcatgatcct gctggagatg 3181 gctccgatgg ataaccagcg ccgcttaagt caggaacagc tacagcacgc ccagcaggtt 3241 actgcccgtg atttagtgcg cggcctggca catgagatta aaaatccgct tggcggttta 3301 cgtggcgcgg cgcagctgct cagcaaagcg ttacctgacc catcactact cgaatatacc 3361 aaagtgatta tcgaacaggc ggaccggctg cgaaatctgg tcgaccgtct gttggggccg 3421 cagctgcccg gtacgcgcgt taccgaaagt attcacaaag tggctgaacg cgtggtaacg 3481 ctggtgtcga tggaactgcc ggacaacgtg cggttgattc gtgattacga tcccagccta 3541 ccggaactgg cgcacgaccc ggatcaaatt gaacaggtct tgctgaatat tgtgcgcaat 3601 gcgctacagg cgctggggcc ggaaggcggt gaaatcattc tgcgtacccg caccgcgttt 3661 caactgacct tacacggcga gcgctaccgg ctggcggcgc ggattgatgt ggaagataac 3721 gggccgggca ttccgcctca tttgcaggat acgctgtttt acccgatggt cagcggccgc 3781 gaaggtggca ccgggcttgg cttatccatc gctcgtaatt tgattgatca gcattcaggc 3841 aaaattgaat ttaccagttg gccagggcat accgagttct cggtttacct gcctatcagg 3901 aaataaccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 3961 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 4021 ctttctgcgt ttatacactg gaaacatcaa ggtcg //