LOCUS BBa_K2066117 3994 bp DNA linear 01-Jun-2024 DEFINITION Synthetic Enhancer: 2x TetO Binding Cassette + NRII on UNS ACCESSION BBa_K2066117 VERSION BBa_K2066117.1 FEATURES Location/Qualifiers misc_feature 1..40 /label=BBa_K2066018 misc_feature 41..87 /label=BBa_K2066543 misc_feature 88..175 /label=BBa_K2066532 promoter 176..205 /label=BBa_K2066544 misc_feature 206..227 /label=BBa_K2066545 misc_feature 228..370 /label=BBa_K2066547 CDS 372..1865 /label=BBa_K2066546 misc_feature 1866..2579 /label=BBa_K2066518 stem_loop 2591..2634 /label=stem_loop misc_feature 2580..2659 /label=BBa_B0010 terminator 2580..2659 /label=BBa_B0010 stem_loop 2675..2694 /label=T7 TE misc_feature 2701..2701 /label=stop polyA_site 2695..2708 /label=polya misc_feature 2668..2708 /label=BBa_B0012 terminator 2668..2708 /label=BBa_B0012 terminator 2580..2708 /label=BBa_B0015 misc_feature 2709..2728 /label=BBa_K2066539 misc_feature 2729..2768 /label=BBa_K2066018 misc_feature 2729..2768 /label=BBa_K2066018 promoter 2729..2795 /label=BBa_K2066126 CDS 2796..3845 /label=BBa_K2066538 stem_loop 3857..3900 /label=stem_loop misc_feature 3846..3925 /label=BBa_B0010 terminator 3846..3925 /label=BBa_B0010 stem_loop 3941..3960 /label=T7 TE misc_feature 3967..3967 /label=stop polyA_site 3961..3974 /label=polya misc_feature 3934..3974 /label=BBa_B0012 terminator 3934..3974 /label=BBa_B0012 terminator 3846..3974 /label=BBa_B0015 misc_feature 3975..3994 /label=BBa_K2066540 ORIGIN 1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc tcacaattgc accaacatgg 61 tgcttaatgt ttccattgaa gcactatatt ggtgcaacat tgtcttcgac gctagcagcg 121 cgcggagatt ctagcccctt ttcgaggaac gtccccatga taacgccttt taggggcaat 181 ttaaaagttg gcacagattt cgctttatct tttttaattg tgagcgctca caattaagct 241 tggcgaaggt ggcaccgggc ttggcttatc catcgctcgt aatttgattg atcagcattc 301 aggcaaaatt gaatttacca gttggccagg gcataccgag ttctcggttt acctgcctat 361 caggaaataa acgtttatgc aacgagggat agtctgggta gtcgatgacg atagttccat 421 ccgttgggtg cttgaacgtg cgctcgctgg ggcaggttta acctgtacga cgtttgagaa 481 cggcgcagaa gtgctggagg cgctggcgag caaaacgccg gatgtgctgc tttcagatat 541 ccgtatgccg ggaatggacg ggctggcgct gctcaagcag attaaacagc gccatccaat 601 gcttccggtc atcattatga ccgcacattc cgatctggat gctgccgtca gcgcctatca 661 acaaggggcg tttgattatc tgcccaaacc gtttgatatc gacgaagcag tggcgctggt 721 tgagcgcgct atcagtcatt accaggaaca gcagcagccg cgtaatgttc agcttaacgg 781 cccaacgacc gatatcatcg gcgaagcgcc agccatgcag gacgtgttcc gtattatcgg 841 tcggctttcg cgttcttcta ttagcgtgct gattaacggc gaatccggca ccggtaaaga 901 actggtcgct catgccctgc atcgccacag tccgcgcgcc aaagcgccgt ttatcgcgct 961 gaatatggca gctatcccaa aagatttgat cgaatcagaa ctgtttggcc acgagaaagg 1021 cgcgtttact ggcgcgaata ccattcgtca ggggcgtttt gaacaggccg atggcggtac 1081 attattcctc gacgaaattg gtgatatgcc gctggatgtg cagacgcgtt tgctgcgcgt 1141 gctggcagac ggtcagtttt accgcgttgg cggctatgcg ccggtgaaag tggatgtgcg 1201 gattatcgct gccactcacc agaatctcga acagcgagtg caggaaggta agttccgtga 1261 ggatctgttc caccgcctga acgttatccg cgttcatctg ccgccgctgc gcgaacgtcg 1321 ggaagatatt ccccgtctgg cgcgccattt tttacaggtt gccgcgcgcg aactgggcgt 1381 agaagcgaag ttactgcatc cggaaaccga agctgctctg acgcgtctgg cgtggccagg 1441 caacgtgcgc cagctggaaa acacctgccg ctggctaacg gtgatggccg ccgggcagga 1501 agtgttgatt caggatttgc ccggcgaact gtttgaatca acggttgcgg agagtacttc 1561 gcaaatgcaa ccggacagct gggcgacgct tcttgcgcag tgggcagaca gagcgctgcg 1621 ttccggtcat caaaatctgc tttccgaagc gcagccagag ctggagcgga cgttactgac 1681 gaccgcgttg cgacatacgc aggggcataa acaggaagcg gcgcggctac tcggctgggg 1741 ccgcaacacc ctgacgcgta agttaaaaga gctggggatg gagtgattca cagcttgtgt 1801 gtaaagattg attattgagc gcaaattgct ggtattttac gctttactgt tccaggagga 1861 aaaaaatggt gagcaagggc gaagaagata acatggccat catcaaggag ttcatgcgct 1921 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg 1981 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc 2041 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga 2101 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg 2161 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccttgc 2221 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc 2281 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg 2341 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg 2401 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca 2461 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt 2521 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaataac 2581 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 2641 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 2701 cgtttatagc actgaaggtc ctcaatcggg taggccggag caggtgagtc gctctccaac 2761 gtgaagtttg tcagctatct gtagcccatc tctgcatggc aacaggcacg cagcccgatg 2821 ctgggcagat cctcaactcg ctgattaaca gtattttgtt aatcgatgac aacctggcga 2881 tccattacgc caaccctgcc gcgcaacaac tgctcgccca aagctcccgc aaattgtttg 2941 gtacaccgtt accggaactg ttgagctact tctcattaaa tatcgagctg atgcaagaaa 3001 gtctggaggc ggggcaaggt tttaccgata acgaagtgac gctggtcatc gacgggcgct 3061 cgcatatcct ttctgtgacg gcccagcgta tgccggacgg catgatcctg ctggagatgg 3121 ctccgatgga taaccagcgc cgcttaagtc aggaacagct acagcacgcc cagcaggtta 3181 ctgcccgtga tttagtgcgc ggcctggcac atgagattaa aaatccgctt ggcggtttac 3241 gtggcgcggc gcagctgctc agcaaagcgt tacctgaccc atcactactc gaatatacca 3301 aagtgattat cgaacaggcg gaccggctgc gaaatctggt cgaccgtctg ttggggccgc 3361 agctgcccgg tacgcgcgtt accgaaagta ttcacaaagt ggctgaacgc gtggtaacgc 3421 tggtgtcgat ggaactgccg gacaacgtgc ggttgattcg tgattacgat cccagcctac 3481 cggaactggc gcacgacccg gatcaaattg aacaggtctt gctgaatatt gtgcgcaatg 3541 cgctacaggc gctggggccg gaaggcggtg aaatcattct gcgtacccgc accgcgtttc 3601 aactgacctt acacggcgag cgctaccggc tggcggcgcg gattgatgtg gaagataacg 3661 ggccgggcat tccgcctcat ttgcaggata cgctgtttta cccgatggtc agcggccgcg 3721 aaggtggcac cgggcttggc ttatccatcg ctcgtaattt gattgatcag cattcaggca 3781 aaattgaatt taccagttgg ccagggcata ccgagttctc ggtttacctg cctatcagga 3841 aataaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 3901 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 3961 tttctgcgtt tatacactgg aaacatcaag gtcg //