LOCUS BBa_K2066121 4083 bp DNA linear 26-May-2024 DEFINITION Synthetic Enhancer Project: 2X TetO Binding Cassette(55aS) + NRII + TetR + sfGFP on UNS ACCESSION BBa_K2066121 VERSION BBa_K2066121.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=BBa_K2066541 misc_feature 56..63 /label=Scar promoter 21..55 /label=J23100 misc_feature 84..103 /label=TetR 1 misc_feature 64..83 /label=TetR 1 misc_feature 64..103 /label=R0040 (2x TetR 1) promoter 21..103 /label=BBa_I739101 misc_feature 104..123 /label=BBa_K2066542 misc_feature 124..170 /label=BBa_K2066543 misc_feature 171..258 /label=BBa_K2066532 promoter 259..288 /label=BBa_K2066544 misc_feature 289..310 /label=BBa_K2066545 misc_feature 311..453 /label=BBa_K2066547 CDS 455..1948 /label=BBa_K2066546 misc_feature 1949..2668 /label=BBa_K2066509 stem_loop 2680..2723 /label=stem_loop misc_feature 2669..2748 /label=BBa_B0010 terminator 2669..2748 /label=BBa_B0010 stem_loop 2764..2783 /label=T7 TE misc_feature 2790..2790 /label=stop polyA_site 2784..2797 /label=polya misc_feature 2757..2797 /label=BBa_B0012 terminator 2757..2797 /label=BBa_B0012 terminator 2669..2797 /label=BBa_B0015 misc_feature 2798..2817 /label=BBa_K2066539 misc_feature 2818..2857 /label=BBa_K2066018 promoter 2818..2884 /label=BBa_K2066126 CDS 2885..3934 /label=BBa_K2066538 stem_loop 3946..3989 /label=stem_loop misc_feature 3935..4014 /label=BBa_B0010 terminator 3935..4014 /label=BBa_B0010 stem_loop 4030..4049 /label=T7 TE misc_feature 4056..4056 /label=stop polyA_site 4050..4063 /label=polya misc_feature 4023..4063 /label=BBa_B0012 terminator 4023..4063 /label=BBa_B0012 terminator 3935..4063 /label=BBa_B0015 misc_feature 4064..4083 /label=BBa_K2066540 ORIGIN 1 gctgggagtt cgtagacgga ttgacggcta gctcagtcct aggtacagtg ctagctacta 61 gagtccctat cagtgataga gattccctat cagtgataga gataacaaac gcagaatcca 121 agctcacaat tgcaccaaca tggtgcttaa tgtttccatt gaagcactat attggtgcaa 181 cattgtcttc gacgctagca gcgcgcggag attctagccc cttttcgagg aacgtcccca 241 tgataacgcc ttttaggggc aatttaaaag ttggcacaga tttcgcttta tcttttttaa 301 ttgtgagcgc tcacaattaa gcttggcgaa ggtggcaccg ggcttggctt atccatcgct 361 cgtaatttga ttgatcagca ttcaggcaaa attgaattta ccagttggcc agggcatacc 421 gagttctcgg tttacctgcc tatcaggaaa taaacgttta tgcaacgagg gatagtctgg 481 gtagtcgatg acgatagttc catccgttgg gtgcttgaac gtgcgctcgc tggggcaggt 541 ttaacctgta cgacgtttga gaacggcgca gaagtgctgg aggcgctggc gagcaaaacg 601 ccggatgtgc tgctttcaga tatccgtatg ccgggaatgg acgggctggc gctgctcaag 661 cagattaaac agcgccatcc aatgcttccg gtcatcatta tgaccgcaca ttccgatctg 721 gatgctgccg tcagcgccta tcaacaaggg gcgtttgatt atctgcccaa accgtttgat 781 atcgacgaag cagtggcgct ggttgagcgc gctatcagtc attaccagga acagcagcag 841 ccgcgtaatg ttcagcttaa cggcccaacg accgatatca tcggcgaagc gccagccatg 901 caggacgtgt tccgtattat cggtcggctt tcgcgttctt ctattagcgt gctgattaac 961 ggcgaatccg gcaccggtaa agaactggtc gctcatgccc tgcatcgcca cagtccgcgc 1021 gccaaagcgc cgtttatcgc gctgaatatg gcagctatcc caaaagattt gatcgaatca 1081 gaactgtttg gccacgagaa aggcgcgttt actggcgcga ataccattcg tcaggggcgt 1141 tttgaacagg ccgatggcgg tacattattc ctcgacgaaa ttggtgatat gccgctggat 1201 gtgcagacgc gtttgctgcg cgtgctggca gacggtcagt tttaccgcgt tggcggctat 1261 gcgccggtga aagtggatgt gcggattatc gctgccactc accagaatct cgaacagcga 1321 gtgcaggaag gtaagttccg tgaggatctg ttccaccgcc tgaacgttat ccgcgttcat 1381 ctgccgccgc tgcgcgaacg tcgggaagat attccccgtc tggcgcgcca ttttttacag 1441 gttgccgcgc gcgaactggg cgtagaagcg aagttactgc atccggaaac cgaagctgct 1501 ctgacgcgtc tggcgtggcc aggcaacgtg cgccagctgg aaaacacctg ccgctggcta 1561 acggtgatgg ccgccgggca ggaagtgttg attcaggatt tgcccggcga actgtttgaa 1621 tcaacggttg cggagagtac ttcgcaaatg caaccggaca gctgggcgac gcttcttgcg 1681 cagtgggcag acagagcgct gcgttccggt catcaaaatc tgctttccga agcgcagcca 1741 gagctggagc ggacgttact gacgaccgcg ttgcgacata cgcaggggca taaacaggaa 1801 gcggcgcggc tactcggctg gggccgcaac accctgacgc gtaagttaaa agagctgggg 1861 atggagtgat tcacagcttg tgtgtaaaga ttgattattg agcgcaaatt gctggtattt 1921 tacgctttac tgttccagga ggaaaaaaat gcgtaaaggc gaagagctgt tcactggtgt 1981 cgtccctatt ctggtggaac tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg 2041 cgagggtgaa ggtgacgcaa ctaatggtaa actgacgctg aagttcatct gtactactgg 2101 taaactgccg gtaccttggc cgactctggt aacgacgctg acttatggtg ttcagtgctt 2161 tgctcgttat ccggaccata tgaagcagca tgacttcttc aagtccgcca tgccggaagg 2221 ctatgtgcag gaacgcacga tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga 2281 agtgaaattt gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa 2341 agaagacggc aatatcctgg gccataagct ggaatacaat tttaacagcc acaatgtgta 2401 cattaccgca gataaacaaa aaaatggcat taaagcgaat ttcaaaattc gccacaacgt 2461 ggaggatggc agcgtgcagc tggctgatca ctaccagcaa aacactccaa tcggtgatgg 2521 tcctgttctg ctgccagaca atcactatct gagcacgcaa agcgttctgt ctaaagatcc 2581 gaacgagaaa cgcgatcata tggttctgct ggagttcgta accgcagcgg gcatcacgca 2641 tggtatggat gaactgtaca aatgatgacc aggcatcaaa taaaacgaaa ggctcagtcg 2701 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 2761 actggctcac cttcgggtgg gcctttctgc gtttatagca ctgaaggtcc tcaatcgggt 2821 aggccggagc aggtgagtcg ctctccaacg tgaagtttgt cagctatctg tagcccatct 2881 ctgcatggca acaggcacgc agcccgatgc tgggcagatc ctcaactcgc tgattaacag 2941 tattttgtta atcgatgaca acctggcgat ccattacgcc aaccctgccg cgcaacaact 3001 gctcgcccaa agctcccgca aattgtttgg tacaccgtta ccggaactgt tgagctactt 3061 ctcattaaat atcgagctga tgcaagaaag tctggaggcg gggcaaggtt ttaccgataa 3121 cgaagtgacg ctggtcatcg acgggcgctc gcatatcctt tctgtgacgg cccagcgtat 3181 gccggacggc atgatcctgc tggagatggc tccgatggat aaccagcgcc gcttaagtca 3241 ggaacagcta cagcacgccc agcaggttac tgcccgtgat ttagtgcgcg gcctggcaca 3301 tgagattaaa aatccgcttg gcggtttacg tggcgcggcg cagctgctca gcaaagcgtt 3361 acctgaccca tcactactcg aatataccaa agtgattatc gaacaggcgg accggctgcg 3421 aaatctggtc gaccgtctgt tggggccgca gctgcccggt acgcgcgtta ccgaaagtat 3481 tcacaaagtg gctgaacgcg tggtaacgct ggtgtcgatg gaactgccgg acaacgtgcg 3541 gttgattcgt gattacgatc ccagcctacc ggaactggcg cacgacccgg atcaaattga 3601 acaggtcttg ctgaatattg tgcgcaatgc gctacaggcg ctggggccgg aaggcggtga 3661 aatcattctg cgtacccgca ccgcgtttca actgacctta cacggcgagc gctaccggct 3721 ggcggcgcgg attgatgtgg aagataacgg gccgggcatt ccgcctcatt tgcaggatac 3781 gctgttttac ccgatggtca gcggccgcga aggtggcacc gggcttggct tatccatcgc 3841 tcgtaatttg attgatcagc attcaggcaa aattgaattt accagttggc cagggcatac 3901 cgagttctcg gtttacctgc ctatcaggaa ataaccaggc atcaaataaa acgaaaggct 3961 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 4021 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atacactgga aacatcaagg 4081 tcg //